公开(公告)号：US20110162114A1

公开(公告)日：2011-06-30

申请号：US12997744

申请日：2009-06-12

申请人： Simon Geir Moller ,  Nam-Hai Chua ,  Jodi Maple

发明人： Simon Geir Moller ,  Nam-Hai Chua ,  Jodi Maple

IPC分类号： A01H5/00 ,  C12N5/10 ,  C12Q1/04 ,  C12P21/00 ,  C12N9/00 ,  C12N15/63 ,  C07K14/00 ,  C07K2/00 ,  C07K16/00

CPC分类号： C12N15/8214

摘要： The invention relates to a method for producing a transformed plant cell. More particularly, the method involves the transformation of a plant cell with a Transformation Cassette which is targeted to plant plastids and which comprises a selection gene, for example isopentenyl transferase (IPT), and a transgene. After selection for transformed plastids, expression of a recombinase is induced in the plant cell, which leads to the excision of the selection gene from the plastid and the expression of the transgene in the plastid. The invention also provides cells and plants comprising the Transformation Cassette.

摘要翻译： 本发明涉及一种生产转化植物细胞的方法。 更具体地，该方法涉及用靶向植物质体的转化盒转化植物细胞，其包含选择基因，例如异戊烯基转移酶（IPT）和转基因。 在选择转化质体后，在植物细胞中诱导重组酶的表达，这导致选择基因从质体中切除和转化子在质体中的表达。 本发明还提供包含转化盒的细胞和植物。

公开(公告)号：US06723896B1

公开(公告)日：2004-04-20

申请号：US09439534

申请日：1999-11-12

申请人： Simon Geir Moller ,  Jianru Zuo ,  Nam-Hai Chua

发明人： Simon Geir Moller ,  Jianru Zuo ,  Nam-Hai Chua

IPC分类号： C12N1582

CPC分类号： C12N15/8237 ,  C12N15/8238

摘要： Disclosed is an inducible promoter system in conjunction with a site-specific recombination system which allows (i) specific activation of transgenes at specific times or (ii) excision and removal of transgenes (e.g., antibiotic resistance markers) from transgenic plants. These “suicide” gene cassettes, including the recombination system itself, can be evicted from the plant genome once their function has been exerted. The system is based on the ability to temporally and spatially induce the expression of CRE recombinase which then binds to directly repeated lox sites flanking the transgene in question leading to the precise excision of the gene cassette. Also disclosed is a method to activate an inverted, and therefore silent, transgene by placing two lox sites in opposite orientations flanking the transgene. This results in inversion of the intervening DNA fragment in the presence of CRE recombinase. This activation can be timed by placing the CRE recombinase under the control of an inducible promoter.

摘要翻译： 公开了与位点特异性重组系统结合的诱导型启动子系统，其允许（i）在特定时间特异性转化转基因，或（ii）从转基因植物切除和除去转基因（例如抗生素抗性标记）。 这些“自杀”基因盒，包括重组系统本身，一旦发挥作用就可以从植物基因组中被驱逐出去。 该系统基于时间和空间诱导CRE重组酶表达的能力，其然后结合直接重复的涉及转基因侧翼的lox位点，从而导致基因盒的精确切除。 还公开了通过将两个lox位点放置在转基因侧翼的相反方向上来激活倒置的，因此沉默的转基因的方法。 这导致在CRE重组酶存在下插入的DNA片段的倒置。 可以通过将CRE重组酶置于诱导型启动子的控制下来定时进行该激活。