-
1.
公开(公告)号:US06344341B1
公开(公告)日:2002-02-05
申请号:US09446920
申请日:1999-12-30
申请人: Sirkka Keränen , Jaana Toikkanen , Ville Tieaho , Hans Söderlund
发明人: Sirkka Keränen , Jaana Toikkanen , Ville Tieaho , Hans Söderlund
IPC分类号: C12P2106
摘要: This invention relates to recombinant-DNA-technology. Specifically this invention relates to new recombinant yeast cells transformed with SEB1 gene. Yeast cells transformed with several copies of SEB1 gene, or overexpressing the Seb1 protein by some other means, have an increased capacity to produce secreted foreign or endogenous proteins. Further, said new recombinant cells, when transformed with genes expressing suitable hydrolytic enzymes can utilize appropriate macromolecular compounds more efficiently, which results in increased cell mass production and/or more versatile utilization of the compounds in relevant biotechnical applications.
摘要翻译: 本发明涉及重组DNA技术。 具体地说,本发明涉及用SEB1基因转化的新的重组酵母细胞。 用几个拷贝的SEB1基因转化的酵母细胞,或通过其他方法过表达Seb1蛋白,具有增加产生分泌的外源或内源蛋白质的能力。 此外,当用表达合适的水解酶的基因转化时,所述新的重组细胞可以更有效地利用合适的大分子化合物,这导致在相关生物技术应用中化合物增加的细胞大量产生和/或更通用的利用。
-
公开(公告)号:US06582944B1
公开(公告)日:2003-06-24
申请号:US09184965
申请日:1999-01-08
申请人: Johan Hallborn , Merja Penttilä , Heikki Ojamo , Mats Walfridsson , Ulla Airaksinen , Sirkka Keränen , Bärbel Hahn-Hägerdal
发明人: Johan Hallborn , Merja Penttilä , Heikki Ojamo , Mats Walfridsson , Ulla Airaksinen , Sirkka Keränen , Bärbel Hahn-Hägerdal
IPC分类号: C12P706
CPC分类号: C12P19/02 , C12N9/0004 , C12N9/0006 , C12N15/52 , C12N15/81 , C12N15/905 , C12P7/06 , C12P7/18 , C12P7/40 , Y02E50/17 , Y02P20/582
摘要: This invention relates to recombinant-DNA-technology. Specifically this invention relates to new recombinant yeast strains transformed with xylose reductase and/or xylitol dehydrogenase enzyme genes. A yeast strain transformed with the xylose reductase gene is capable of reducing xylose to xylitol and consequently of producing xylitol in vivo. If both of these genes are transformed into a yeast strain, the resultant strain is capable of producing ethanol on xylose containing medium during fermentation. Further, the said new yeast strains are capable of expressing the said two enzymes. Xylose reductase produced by these strains can be used in an enzymatic process for the production of xylitol in vitro.
摘要翻译: 本发明涉及重组DNA技术。 具体地说,本发明涉及用木糖还原酶和/或木糖醇脱氢酶基因转化的新的重组酵母菌株。 用木糖还原酶基因转化的酵母菌株能够将木糖还原成木糖醇,从而在体内产生木糖醇。 如果将这两个基因转化成酵母菌株,则所得菌株能够在发酵期间在含有木糖的培养基上产生乙醇。 此外,所述新酵母菌株能够表达所述两种酶。 由这些菌株产生的木糖还原酶可用于体外生产木糖醇的酶法。
-