Increased production of secreted proteins by recombinant yeast cells
    1.
    发明授权
    Increased production of secreted proteins by recombinant yeast cells 有权
    通过重组酵母细胞增加分泌蛋白质的产生

    公开(公告)号:US06344341B1

    公开(公告)日:2002-02-05

    申请号:US09446920

    申请日:1999-12-30

    IPC分类号: C12P2106

    CPC分类号: C12N15/81 C07K14/39

    摘要: This invention relates to recombinant-DNA-technology. Specifically this invention relates to new recombinant yeast cells transformed with SEB1 gene. Yeast cells transformed with several copies of SEB1 gene, or overexpressing the Seb1 protein by some other means, have an increased capacity to produce secreted foreign or endogenous proteins. Further, said new recombinant cells, when transformed with genes expressing suitable hydrolytic enzymes can utilize appropriate macromolecular compounds more efficiently, which results in increased cell mass production and/or more versatile utilization of the compounds in relevant biotechnical applications.

    摘要翻译: 本发明涉及重组DNA技术。 具体地说,本发明涉及用SEB1基因转化的新的重组酵母细胞。 用几个拷贝的SEB1基因转化的酵母细胞,或通过其他方法过表达Seb1蛋白,具有增加产生分泌的外源或内源蛋白质的能力。 此外,当用表达合适的水解酶的基因转化时,所述新的重组细胞可以更有效地利用合适的大分子化合物,这导致在相关生物技术应用中化合物增加的细胞大量产生和/或更通用的利用。