公开(公告)号：US20190203275A1

公开(公告)日：2019-07-04

申请号：US15565637

申请日：2016-04-04

Applicant: Spatial Transcriptomics AB ,  ILLUMINA, INC.

Inventor： JONAS FRISÉN ,  PATRIK STÅHL ,  JOAKIM LUNDEBERG ,  GORDON M. CANN ,  LEILA BAZARGAN ,  ALEX ARAVANIS

IPC: C12Q1/6834 ,  C12Q1/6841 ,  C12Q1/6876 ,  C12Q1/6874

Abstract: A method for spatially tagging nucleic acids of a biological specimen, including steps of (a) providing a solid support comprising different nucleic acid probes that are randomly located on the solid support, wherein the different nucleic acid probes each includes a barcode sequence that differs from the barcode sequence of other randomly located probes on the solid support; (b) performing a nucleic acid detection reaction on the solid support to locate the barcode sequences on the solid support; (c) contacting a biological specimen with the solid support that has the randomly located probes; (d) hybridizing the randomly located probes to target nucleic acids from portions of the biological specimen; and (e) modifying the randomly located probes that are hybridized to the target nucleic acids, thereby producing modified probes that include the barcode sequences and a target specific modification, thereby spatially tagging the nucleic acids of the biological specimen.

公开(公告)号：US20190024153A1

公开(公告)日：2019-01-24

申请号：US16042950

申请日：2018-07-23

Applicant: SPATIAL TRANSCRIPTOMICS AB

Inventor： JONAS FRISEN ,  PATRIK STÅHL ,  JOAKIM LUNDEBERG

IPC: C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6841

Abstract: Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array includes a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

公开(公告)号：US20190024154A1

公开(公告)日：2019-01-24

申请号：US16043038

申请日：2018-07-23

Applicant: SPATIAL TRANSCRIPTOMICS AB

Inventor： JONAS FRISEN ,  PATRIK STÅHL ,  JOAKIM LUNDEBERG

IPC: C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6841

Abstract: Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.

公开(公告)号：US20190017106A1

公开(公告)日：2019-01-17

申请号：US16013654

申请日：2018-06-20

Applicant: SPATIAL TRANSCRIPTOMICS AB

Inventor： JONAS FRISEN ,  PATRIK STÅHL ,  JOAKIM LUNDEBERG

IPC: C12Q1/6837 ,  C12Q1/6844 ,  C12Q1/6841

Abstract: Localized detection of RNA in a tissue sample that includes cells is accomplished on an array. The array include a number of features on a substrate. Each feature includes a different capture probe immobilized such that the capture probe has a free 3′ end. Each feature occupies a distinct position on the array and has an area of less than about 1 mm2. Each capture probe is a nucleic acid molecule, which includes a positional domain including a nucleotide sequence unique to a particular feature, and a capture domain including a nucleotide sequence complementary to the RNA to be detected. The capture domain can be at a position 3′ of the positional domain.