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公开(公告)号：US5175271A
公开(公告)日：1992-12-29
申请号：US825695
申请日：1992-01-27
申请人： Stanley Thomas , Lowell Tilzer , Ruben Moreno
发明人： Stanley Thomas , Lowell Tilzer , Ruben Moreno
CPC分类号： C12N15/1003 , C12N15/1006 , C12Q1/6806 , Y10S435/803
摘要： The invention describes a method for extracting DNA from a sample. It involves contacting a sample with a separation reagent which permits differentiated solvation of DNA and protein. By adding a gel polymer, such as a silical gel polymer to the mixture of sample and separating reagent followed by agitation via, e.g., centrifugation, the DNA and protein are separated, with the gel acting as a block to prevent contamination of the DNA phase by the protein. Higher yields of DNA are obtained as compared to methodologies where the gel is not used. Additionally, the problems associated with the physical contact of the solvents, which are frequently carcinogens, are avoided. Also taught are kits which can be used in connection with the inventive method.
摘要翻译： 本发明描述了从样品中提取DNA的方法。 它包括使样品与分离试剂接触，分离试剂允许DNA和蛋白质的分化溶剂化。 通过向样品和分离试剂的混合物中加入诸如硅胶凝胶聚合物的凝胶聚合物，然后通过例如离心进行搅拌，分离DNA和蛋白质，凝胶充当块，以防止DNA相的污染 由蛋白质。 与不使用凝胶的方法相比，获得更高的DNA产量。 此外，避免了与经常致癌的溶剂的物理接触相关的问题。 还教导了可以结合本发明的方法使用的试剂盒。
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公开(公告)号：US5106966A
公开(公告)日：1992-04-21
申请号：US381588
申请日：1989-07-10
申请人： Stanley Thomas , Lowell Tilzer , Ruben Moreno
发明人： Stanley Thomas , Lowell Tilzer , Ruben Moreno
CPC分类号： C12N15/1003 , C12Q1/6806 , Y10S435/803
摘要： The invention describes a method for extracting DNA from a sample. It involves contacting a sample with a separation reagent which permits differentiated solvation of DNA and protein. By adding a gel polymer, such as a polyester gel polymer to the mixture of sample and separating reagent followed by agitation via, e.g., centrifugation, the DNA and protein are separated, with the gel acting as a block to prevent contamination of the DNA phase by the protein. Higher yields of DNA are obtained as compared to methodologies where the gel is not used. Additionally, the problems associated with the physical contact of the solvents, which are frequently carcinogens, are avoided. Also taught are kits which can be used in connection with the inventive method.
摘要翻译： 本发明描述了从样品中提取DNA的方法。 它包括使样品与分离试剂接触，分离试剂允许DNA和蛋白质的分化溶剂化。 通过将诸如聚酯凝胶聚合物的凝胶聚合物添加到样品和分离试剂的混合物中，然后通过例如离心进行搅拌，分离DNA和蛋白质，凝胶充当块，以防止DNA相的污染 由蛋白质。 与不使用凝胶的方法相比，获得更高的DNA产量。 此外，避免了与经常致癌的溶剂的物理接触相关的问题。 还教导了可以结合本发明的方法使用的试剂盒。
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公开(公告)号：US20120307304A1
公开(公告)日：2012-12-06
申请号：US13486538
申请日：2012-06-01
申请人： Ruben Moreno
发明人： Ruben Moreno
IPC分类号： G06K15/02
摘要： This invention relates to the on-site creation of custom articles. Systems and methods of the invention are provided for receiving personalized artwork such as digitally-input freehand drawings and printing the artwork on-site and substantially immediately onto articles of apparel, merchandise, and the like.
摘要翻译： 本发明涉及定制物品的现场创建。 提供本发明的系统和方法用于接收个性化的艺术作品，例如数字输入的徒手绘画，并将艺术品现场并且基本上立即地印刷到服装，商品等上。
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