公开(公告)号：US20070020689A1

公开(公告)日：2007-01-25

申请号：US11489173

申请日：2006-07-19

申请人： Stephen Caracci ,  Volker Eckelt ,  Anthony Frutos ,  Mark Krol ,  Thomas Moore ,  David Pastel ,  Gordon Shedd

发明人： Stephen Caracci ,  Volker Eckelt ,  Anthony Frutos ,  Mark Krol ,  Thomas Moore ,  David Pastel ,  Gordon Shedd

IPC分类号： C40B30/06 ,  G01N33/53 ,  G06F19/00 ,  C12M1/34

CPC分类号： G01N35/028 ,  G01N2035/00158 ,  G01N2035/00356 ,  G01N2035/0097

摘要： A screening system and method are described herein which provide a unique and practical solution for enabling label-free high throughput screening (HTS) to aid in the discovery of new drugs. In one embodiment, the screening system enables direct binding assays to be performed in which a biomolecular interaction of a chemical compound (drug candidate) with a biomolecule (therapeutic target) can be detected using assay volumes and concentrations that are compatible with the current practices of HTS in the pharmaceutical industry. The screening system also enables the detection of bio-chemical interactions that occurr in the wells of a microplate which incorporates biosensors and surface chemistry to immobilize the therapeutic target at the surface of the biosensors. The screening system also includes fluid handling and plate handling devices to help perform automated HTS assays.

摘要翻译： 本文描述了一种筛选系统和方法，其提供用于实现无标记高通量筛选（HTS）以帮助发现新药物的独特且实用的解决方案。 在一个实施方案中，筛选系统使得能够进行直接结合测定，其中化学化合物（药物候选物）与生物分子（治疗靶标）的生物分子相互作用可以使用与当前实践相兼容的测定体积和浓度来检测 HTS在制药行业。 筛选系统还能够检测在微板的孔中发生的生物化学相互作用，其结合生物传感器和表面化学以将治疗靶固定在生物传感器的表面。 筛选系统还包括流体处理和板处理装置，以帮助执行自动HTS测定。