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    Detection of preharvest sprouting in cereal grains
    4.
    发明授权
    Detection of preharvest sprouting in cereal grains 失效
    检测谷物中的收获前发芽

    公开(公告)号：US07074579B1

    公开(公告)日：2006-07-11

    申请号：US09830876

    申请日：1999-11-11

    申请人： John Howard Skerritt

    发明人： John Howard Skerritt

    IPC分类号： G01N33/53

    CPC分类号： G01N33/573 ,  Y10S436/808 ,  Y10S530/866

    摘要： A two-site immunoassay for the qualitative or quantitative detection of alpha-amylase in a test sample, said immunoassay comprising: (1) exposing said test sample to a first (“capture”) antibody or fragment thereof which specifically or preferentially binds to a first epitope on said alpha-amylase, under conditions permitting binding of said first antibody or fragment thereof to alpha-amylase if present, (ii) subsequently exposing bound alpha-amylase, if any, to a second (“detection”) antibody or fragment thereof which specifically or preferentially binds to a second epitope on said alpha-amylase that is distinct from said first epitope, under conditions permitting binding of said second antibody or fragment thereof to said bound alpha-amylase, and (iii) detecting any binding of said second antibody or fragment thereof to said bound alpha-amylase, wherein either of said first or second epitopes is an epitope comprising one or more of the amino acid sequences: IDRLVSIRTRGQIHS (SEQ ID NO:1), CRDDRPYADG (SEQ ID NO:2), VNWVNKVGGS (SEQ ID NO:3) and variants thereof showing ≧80%, more preferably ≧90%, sequence identity. The immunoassay is useful for detecting weather damage (i.e., preharvest sprouting) in cereal grain.

    摘要翻译： 用于在测试样品中定性或定量检测α-淀粉酶的双位点免疫测定法，所述免疫测定法包括：（1）将所述测试样品暴露于第一（“捕获”）抗体或其片段，其特异性或优先结合于 在所述α-淀粉酶上的第一表位，在允许所述第一抗体或其片段与α-淀粉酶（如果存在）结合的条件下，（ii）随后将结合的α-淀粉酶（如果有的话）暴露于第二（“检测”）抗体或片段 其在允许所述第二抗体或其片段与所述结合的α-淀粉酶结合的条件下，特异地或优先地结合所述α-淀粉酶上不同于所述第一表位的第二表位，和（iii）检测所述 第二抗体或其片段与所述结合的α-淀粉酶，其中所述第一或第二表位中的任一个是包含一个或多个氨基酸序列的表位：IDRLVSIRTRGQIHS（SEQ ID NO ：1），CRDDRPYADG（SEQ ID NO：2），VNWVNKVGGS（SEQ ID NO：3）及其变体，显示≥80％，更优选> = 90％的序列同一性。 免疫测定可用于检测谷物中的天气损伤（即，预收获发芽）。