公开(公告)号：US20090257950A1

公开(公告)日：2009-10-15

申请号：US11870217

申请日：2007-10-10

申请人： Stephen G. Sligar ,  Timothy H. Bayburt ,  Mary A. Schuler ,  Natanya R. Civjan ,  Yelena V. Grinkova ,  Ilia G. Denisov ,  Stephen James Grimme

发明人： Stephen G. Sligar ,  Timothy H. Bayburt ,  Mary A. Schuler ,  Natanya R. Civjan ,  Yelena V. Grinkova ,  Ilia G. Denisov ,  Stephen James Grimme

IPC分类号： A61K51/08 ,  A61K9/14 ,  A61K47/42 ,  A61K38/16 ,  A61K38/02 ,  A61K31/7052 ,  A61K31/70 ,  A61K31/7048 ,  A61K31/497 ,  A61K39/395 ,  A61K49/00 ,  A61P43/00

CPC分类号： C07K14/775 ,  C07K14/47

摘要： The membrane scaffold proteins (MSP) of the present invention assemble with hydrophobic or partially hydrophobic proteins to form soluble nanoscale particles which preserve native structure and function; they are improved over liposomes and detergent micelles, both in terms of stability and preservation of biological activity and native conformation. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles, which are robust in terms of integrity and maintenance of biological activity of incorporated proteins, facilitate pharmaceutical and biological research, structure/function correlations, structure determinations, bioseparations, and drug discovery.

摘要翻译： 本发明的膜支架蛋白（MSP）与疏水或部分疏水的蛋白质组合形成可溶性纳米级颗粒，其保留天然结构和功能; 在稳定性和保存生物活性和天然构象方面，它们都比脂质体和洗涤剂胶束得到改善。 在磷脂的存在下，MSP形成纳米视磷脂双层盘，MSP在双层结构域的周边稳定颗粒。 颗粒双层结构允许在溶液中或固体载体上操作掺入的蛋白质，包括用于诸如扫描探针显微镜或表面等离子体共振的表面敏感技术。 在完整性和维持结合蛋白质的生物活性方面坚固的纳米级颗粒有助于药物和生物学研究，结构/功能相关性，结构测定，生物分离和药物发现。

公开(公告)号：US07592008B2

公开(公告)日：2009-09-22

申请号：US11033489

申请日：2005-01-11

申请人： Stephen G. Sligar ,  Timothy H. Bayburt ,  Mary A. Schuler ,  Natanya R. Civjan ,  Ylena V. Grinkova ,  Ilia G. Denisov ,  Stephen James Grimme

发明人： Stephen G. Sligar ,  Timothy H. Bayburt ,  Mary A. Schuler ,  Natanya R. Civjan ,  Ylena V. Grinkova ,  Ilia G. Denisov ,  Stephen James Grimme

IPC分类号： A61K39/00 ,  A61K38/00 ,  A61K39/12 ,  A61K39/02 ,  A61K39/002

CPC分类号： C07K14/775 ,  C07K14/47

摘要： The membrane scaffold proteins (MSP) of the present invention assemble with hydrophobic or partially hydrophobic proteins to form soluble nanoscale particles which preserve native structure and function; they are improved over liposomes and detergent micelles, in terms of stability and preservation of biological activity and native conformation. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles, which are robust in terms of integrity and maintenance of biological activity of incorporated proteins, facilitate pharmaceutical and biological research, structure/function correlations, structure determinations, bioseparations, and drug discovery.

摘要翻译： 本发明的膜支架蛋白（MSP）与疏水或部分疏水的蛋白质组合形成可溶性纳米级颗粒，其保留天然结构和功能; 在稳定性和保存生物活性和天然构象方面，它们比脂质体和洗涤剂胶束得到改善。 在磷脂的存在下，MSP形成纳米视磷脂双层盘，MSP在双层结构域的周边稳定颗粒。 颗粒双层结构允许在溶液中或固体载体上操作掺入的蛋白质，包括用于诸如扫描探针显微镜或表面等离子体共振的表面敏感技术。 在完整性和维持结合蛋白质的生物活性方面坚固的纳米级颗粒有助于药物和生物学研究，结构/功能相关性，结构测定，生物分离和药物发现。