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公开(公告)号：US07691414B2
公开(公告)日：2010-04-06
申请号：US10979506
申请日：2004-11-02
IPC分类号： A61K9/14 , C07K14/00 , C07K14/435
CPC分类号： C07K14/47
摘要： Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. Membrane scaffold proteins (MSP) assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their native structure and function; they are improved over liposomes and detergent micelles. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles facilitate pharmaceutical and biological research, structure/function correlation, structure determination, bioseparation, and drug discovery.
摘要翻译： 膜蛋白难以以重组形式表达，纯化和表征，至少部分是由于其疏水性或部分疏水性质。 膜支架蛋白（MSP）与靶膜或其他疏水或部分疏水的蛋白质或膜片段组装形成可溶性纳米级颗粒，保留其天然结构和功能; 它们比脂质体和洗涤剂胶束改进。 在磷脂的存在下，MSP形成纳米视磷脂双层盘，MSP在双层结构域的周边稳定颗粒。 颗粒双层结构允许在溶液中或固体载体上操作掺入的蛋白质，包括用于诸如扫描探针显微镜或表面等离振子共振的表面敏感技术。 纳米级颗粒促进药物和生物学研究，结构/功能相关性，结构测定，生物分离和药物发现。
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公开(公告)号：US07592008B2
公开(公告)日：2009-09-22
申请号：US11033489
申请日：2005-01-11
申请人： Stephen G. Sligar , Timothy H. Bayburt , Mary A. Schuler , Natanya R. Civjan , Ylena V. Grinkova , Ilia G. Denisov , Stephen James Grimme
发明人： Stephen G. Sligar , Timothy H. Bayburt , Mary A. Schuler , Natanya R. Civjan , Ylena V. Grinkova , Ilia G. Denisov , Stephen James Grimme
IPC分类号： A61K39/00 , A61K38/00 , A61K39/12 , A61K39/02 , A61K39/002
CPC分类号： C07K14/775 , C07K14/47
摘要： The membrane scaffold proteins (MSP) of the present invention assemble with hydrophobic or partially hydrophobic proteins to form soluble nanoscale particles which preserve native structure and function; they are improved over liposomes and detergent micelles, in terms of stability and preservation of biological activity and native conformation. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles, which are robust in terms of integrity and maintenance of biological activity of incorporated proteins, facilitate pharmaceutical and biological research, structure/function correlations, structure determinations, bioseparations, and drug discovery.
摘要翻译： 本发明的膜支架蛋白（MSP）与疏水或部分疏水的蛋白质组合形成可溶性纳米级颗粒，其保留天然结构和功能; 在稳定性和保存生物活性和天然构象方面，它们比脂质体和洗涤剂胶束得到改善。 在磷脂的存在下，MSP形成纳米视磷脂双层盘，MSP在双层结构域的周边稳定颗粒。 颗粒双层结构允许在溶液中或固体载体上操作掺入的蛋白质，包括用于诸如扫描探针显微镜或表面等离子体共振的表面敏感技术。 在完整性和维持结合蛋白质的生物活性方面坚固的纳米级颗粒有助于药物和生物学研究，结构/功能相关性，结构测定，生物分离和药物发现。
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3.发明申请BIOMIMETIC CHEMICAL SENSORS USING NANOELECTRONIC READOUT OF OLFACTORY RECEPTORS 有权使用纳米电子阅读器的生物化学传感器公开(公告)号：US20150065363A1
公开(公告)日：2015-03-05
申请号：US13807323
申请日：2011-06-29
申请人： Alan T. Johnson, JR. , Brett R. Goldsmith , Joseph J. Mitala, JR. , Bohdana Discher , Stephen G. Sligar , Timothy H. Bayburt
发明人： Alan T. Johnson, JR. , Brett R. Goldsmith , Joseph J. Mitala, JR. , Bohdana Discher , Stephen G. Sligar , Timothy H. Bayburt
IPC分类号： G01N33/543
CPC分类号： G01N33/6842 , G01N33/5432 , G01N33/54353 , G01N33/5438 , G01N33/554 , G01N33/6872 , G01N2333/726 , G06F19/28
摘要： The present invention provides biomimetic sensor devices that utilize proteins—such as G-protein coupled receptors—and are useful in high-sensitivity analysis of analyte-containing samples. These sensors may be used to determine the presence or concentration of one or more analytes in a sample. The invention also includes methods of fabricating the devices and methods of using the devices to assay samples.
摘要翻译： 本发明提供利用蛋白质（例如G蛋白偶联受体）的仿生传感器装置，并且可用于含分析物样品的高灵敏度分析。 这些传感器可用于确定样品中一种或多种分析物的存在或浓度。 本发明还包括制造装置的方法和使用该装置来测定样品的方法。
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公开(公告)号：US07575763B2
公开(公告)日：2009-08-18
申请号：US11439458
申请日：2006-05-23
IPC分类号： A61K9/14 , C07K14/00 , C07K14/435
CPC分类号： C07K14/47
摘要： Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. Membrane scaffold proteins (MSP) assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their native structure and function; they are improved over liposomes and detergent micelles. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles facilitate pharmaceutical and biological research, structure/function correlation, structure determination, bioseparation, and drug discovery.
摘要翻译： 膜蛋白难以以重组形式表达，纯化和表征，至少部分是由于其疏水性或部分疏水性质。 膜支架蛋白（MSP）与靶膜或其他疏水或部分疏水的蛋白质或膜片段组装形成可溶性纳米级颗粒，保留其天然结构和功能; 它们比脂质体和洗涤剂胶束改进。 在磷脂的存在下，MSP形成纳米视磷脂双层盘，MSP在双层结构域的周边稳定颗粒。 颗粒双层结构允许在溶液中或固体载体上操作掺入的蛋白质，包括用于诸如扫描探针显微镜或表面等离子体共振的表面敏感技术。 纳米级颗粒促进药物和生物学研究，结构/功能相关性，结构测定，生物分离和药物发现。
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公开(公告)号：US09612240B2
公开(公告)日：2017-04-04
申请号：US13807323
申请日：2011-06-29
申请人： Alan T. Johnson, Jr. , Brett R. Goldsmith , Joseph J. Mitala, Jr. , Bohdana Discher , Stephen G. Sligar , Timothy H. Bayburt
发明人： Alan T. Johnson, Jr. , Brett R. Goldsmith , Joseph J. Mitala, Jr. , Bohdana Discher , Stephen G. Sligar , Timothy H. Bayburt
IPC分类号： G01N33/543 , G01N33/554
CPC分类号： G01N33/6842 , G01N33/5432 , G01N33/54353 , G01N33/5438 , G01N33/554 , G01N33/6872 , G01N2333/726 , G06F19/28
摘要： The present invention provides biomimetic sensor devices that utilize proteins—such as G-protein coupled receptors—and are useful in high-sensitivity analysis of analyte-containing samples. These sensors may be used to determine the presence or concentration of one or more analytes in a sample. The invention also includes methods of fabricating the devices and methods of using the devices to assay samples.
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公开(公告)号：US07662410B2
公开(公告)日：2010-02-16
申请号：US11439466
申请日：2006-05-23
IPC分类号： A61K9/14 , C07K14/00 , C07K14/435
CPC分类号： C07K14/47
摘要： Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. Membrane scaffold proteins (MSP) assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their native structure and function; they are improved over liposomes and detergent micelles. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles facilitate pharmaceutical and biological research, structure/function correlation, structure determination, bioseparation, and drug discovery.
摘要翻译： 膜蛋白难以以重组形式表达，纯化和表征，至少部分是由于其疏水性或部分疏水性质。 膜支架蛋白（MSP）与靶膜或其他疏水或部分疏水的蛋白质或膜片段组装形成可溶性纳米级颗粒，保留其天然结构和功能; 它们比脂质体和洗涤剂胶束改进。 在磷脂的存在下，MSP形成纳米视磷脂双层盘，MSP在双层结构域的周边稳定颗粒。 颗粒双层结构允许在溶液中或固体载体上操作掺入的蛋白质，包括用于诸如扫描探针显微镜或表面等离子体共振的表面敏感技术。 纳米级颗粒促进药物和生物学研究，结构/功能相关性，结构测定，生物分离和药物发现。
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公开(公告)号：US20090257950A1
公开(公告)日：2009-10-15
申请号：US11870217
申请日：2007-10-10
申请人： Stephen G. Sligar , Timothy H. Bayburt , Mary A. Schuler , Natanya R. Civjan , Yelena V. Grinkova , Ilia G. Denisov , Stephen James Grimme
发明人： Stephen G. Sligar , Timothy H. Bayburt , Mary A. Schuler , Natanya R. Civjan , Yelena V. Grinkova , Ilia G. Denisov , Stephen James Grimme
IPC分类号： A61K51/08 , A61K9/14 , A61K47/42 , A61K38/16 , A61K38/02 , A61K31/7052 , A61K31/70 , A61K31/7048 , A61K31/497 , A61K39/395 , A61K49/00 , A61P43/00
CPC分类号： C07K14/775 , C07K14/47
摘要： The membrane scaffold proteins (MSP) of the present invention assemble with hydrophobic or partially hydrophobic proteins to form soluble nanoscale particles which preserve native structure and function; they are improved over liposomes and detergent micelles, both in terms of stability and preservation of biological activity and native conformation. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles, which are robust in terms of integrity and maintenance of biological activity of incorporated proteins, facilitate pharmaceutical and biological research, structure/function correlations, structure determinations, bioseparations, and drug discovery.
摘要翻译： 本发明的膜支架蛋白（MSP）与疏水或部分疏水的蛋白质组合形成可溶性纳米级颗粒，其保留天然结构和功能; 在稳定性和保存生物活性和天然构象方面，它们都比脂质体和洗涤剂胶束得到改善。 在磷脂的存在下，MSP形成纳米视磷脂双层盘，MSP在双层结构域的周边稳定颗粒。 颗粒双层结构允许在溶液中或固体载体上操作掺入的蛋白质，包括用于诸如扫描探针显微镜或表面等离子体共振的表面敏感技术。 在完整性和维持结合蛋白质的生物活性方面坚固的纳米级颗粒有助于药物和生物学研究，结构/功能相关性，结构测定，生物分离和药物发现。
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公开(公告)号：US07083958B2
公开(公告)日：2006-08-01
申请号：US10465789
申请日：2003-06-18
申请人： Stephen G. Sligar , Timothy H. Bayburt , Mary A. Schuler , Natanya R Civjan , Yelena V. Grinkova , Ilia G. Denisov
发明人： Stephen G. Sligar , Timothy H. Bayburt , Mary A. Schuler , Natanya R Civjan , Yelena V. Grinkova , Ilia G. Denisov
IPC分类号： C12N9/00 , C07K14/435
CPC分类号： C07K14/47
摘要： Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. The membrane scaffold proteins (MSP) of the present invention assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their native structure and function; they are improved over liposomes and detergent micelles. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles, which are robust in terms of integrity and maintenance of biological activity of incorporated proteins, facilitate pharmaceutical and biological research, structure/function correlation, structure determination, bioseparation, and drug discovery.
摘要翻译： 膜蛋白难以以重组形式表达，纯化和表征，至少部分是由于其疏水性或部分疏水性质。 本发明的膜支架蛋白（MSP）与靶膜或其他疏水或部分疏水的蛋白质或膜片段组装以形成保护其天然结构和功能的可溶性纳米级颗粒; 它们比脂质体和洗涤剂胶束改进。 在磷脂的存在下，MSP形成纳米视磷脂双层盘，MSP在双层结构域的周边稳定颗粒。 颗粒双层结构允许在溶液中或固体载体上操作掺入的蛋白质，包括用于诸如扫描探针显微镜或表面等离子体共振的表面敏感技术。 在完整性和维持结合蛋白的生物活性方面坚固的纳米级颗粒有助于药物和生物学研究，结构/功能相关性，结构测定，生物分离和药物发现。
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公开(公告)号：US07048949B2
公开(公告)日：2006-05-23
申请号：US09990087
申请日：2001-11-20
IPC分类号： A61K9/14 , C07K14/00 , C07K14/435
CPC分类号： C07K14/47
摘要： Membrane proteins are difficult to express in recombinant form, purify, and characterize, at least in part due to their hydrophobic or partially hydrophobic properties. Membrane scaffold proteins (MSP) assemble with target membrane or other hydrophobic or partially hydrophobic proteins or membrane fragments to form soluble nanoscale particles which preserve their native structure and function; they are improved over liposomes and detergent micelles. In the presence of phospholipid, MSPs form nanoscopic phospholipid bilayer disks, with the MSP stabilizing the particle at the perimeter of the bilayer domain. The particle bilayer structure allows manipulation of incorporated proteins in solution or on solid supports, including for use with such surface-sensitive techniques as scanning probe microscopy or surface plasmon resonance. The nanoscale particles facilitate pharmaceutical and biological research, structure/function correlation, structure determination, bioseparation, and drug discovery.
摘要翻译： 膜蛋白难以以重组形式表达，纯化和表征，至少部分是由于其疏水性或部分疏水性质。 膜支架蛋白（MSP）与靶膜或其他疏水或部分疏水的蛋白质或膜片段组装形成可溶性纳米级颗粒，保留其天然结构和功能; 它们比脂质体和洗涤剂胶束改进。 在磷脂的存在下，MSP形成纳米视磷脂双层盘，MSP在双层结构域的周边稳定颗粒。 颗粒双层结构允许在溶液中或固体载体上操作掺入的蛋白质，包括用于诸如扫描探针显微镜或表面等离子体共振的表面敏感技术。 纳米级颗粒促进药物和生物学研究，结构/功能相关性，结构测定，生物分离和药物发现。
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