公开(公告)号：US06627746B1

公开(公告)日：2003-09-30

申请号：US09084303

申请日：1998-05-26

申请人： Stephen Kohl Doberstein ,  Darren Mark Platt ,  Kimberly Carr Ferguson ,  Andrew Roy Buchman ,  Sheila Akiko Homburger

发明人： Stephen Kohl Doberstein ,  Darren Mark Platt ,  Kimberly Carr Ferguson ,  Andrew Roy Buchman ,  Sheila Akiko Homburger

IPC分类号： C07H2104

CPC分类号： C07K14/43545 ,  A61K38/00

摘要： The present invention relates to C. elegans insulin-like genes and methods for identifying insulin-like genes. The methods provide nucleotide sequences of C. elegans insulin-like genes, amino acid sequences of their encoded proteins, and derivatives (e.g., fragments) and analogs thereof. The invention further relates to fragments (and derivatives and analogs thereof) of insulin-like proteins which comprise one or more domains of an insulin-like protein. Antibodies to an insulin-like protein, and derivatives and analogs thereof, are provided. Methods of production of an insulin-like protein (e.g., by recombinant means), and derivatives and analogs thereof, are provided. Further, methods to identify the biological function of a C. elegans insulin-like gene are provided, including various methods for the functional modification (e.g., overexpression, underexpression, mutation, knock-out) of one or more genes simultaneously. Still further, methods to identify a C. elegans gene which modifies the function of, and/or functions in a downstream pathway from, an insulin-like gene are provided.

公开(公告)号：US06781028B1

公开(公告)日：2004-08-24

申请号：US09332522

申请日：1999-06-14

申请人： Michael R. Costa ,  Stephen K. Doberstein ,  Sarah L. Elson ,  Kimberly Carr Ferguson ,  Sheila Akiko Homburger

发明人： Michael R. Costa ,  Stephen K. Doberstein ,  Sarah L. Elson ,  Kimberly Carr Ferguson ,  Sheila Akiko Homburger

IPC分类号： G01N3300

CPC分类号： C07K14/4705 ,  A01K67/0336 ,  A01K2217/05 ,  A01K2217/075 ,  C07K2319/00

摘要： Drosophila melanogaster and C. elegans that have been genetically modified to express or mis-express proteins involved in the sterol regulatory element binding protein (SREBP) pathway are described. These genetically modified animal models have identifiable phenotypes that make them useful in assays for studying lipid metabolism, other genes implicated in lipid metabolism, and compounds capable of modulating lipid metabolism pathways. Methods for studying lipid metabolism in living nematodes using fluorescently-labelled fatty acid conjugates, such BODITY™ fatty acid conjugates, are also described. Novel SREBP pathway nucleic acid and protein sequences are also described.

摘要翻译： 描述了已经被遗传修饰以表达或错误表达参与固醇调节元件结合蛋白（SREBP）途径的蛋白质的黑腹果蝇和秀丽隐杆线虫。 这些遗传修饰的动物模型具有可识别的表型，使其可用于研究脂质代谢，其他涉及脂质代谢的基因和能够调节脂质代谢途径的化合物。 还描述了使用荧光标记的脂肪酸共轭物（例如BODITY TM脂肪酸共轭物）研究活体线虫脂质代谢的方法。 还描述了新的SREBP途径核酸和蛋白质序列。

公开(公告)号：US20100112566A1

公开(公告)日：2010-05-06

申请号：US12442372

申请日：2007-09-24

申请人： Kyle A. Edgar ,  Kimberly Carr Ferguson ,  Monique Nicoll ,  Christopher G. Winter

发明人： Kyle A. Edgar ,  Kimberly Carr Ferguson ,  Monique Nicoll ,  Christopher G. Winter

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： G01N33/574 ,  C12Q1/6886 ,  C12Q2600/136 ,  C12Q2600/178 ,  G01N33/566 ,  G01N2333/4706

摘要： Human VIPR1 genes are identified as modulators of the E2F/RB pathway, and thus are therapeutic targets for disorders associated with defective E2F/RB function. Methods for identifying modulators of E2F/RB, comprising screening for agents that modulate the activity of VIPR1 are provided.

摘要翻译： 人类VIPR1基因被鉴定为E2F / RB途径的调节因子，因此是与缺陷型E2F / RB功能相关的疾病的治疗靶点。 提供用于鉴定E2F / RB调节剂的方法，包括筛选调节VIPR1活性的试剂。