公开(公告)号：US06781028B1

公开(公告)日：2004-08-24

申请号：US09332522

申请日：1999-06-14

申请人： Michael R. Costa ,  Stephen K. Doberstein ,  Sarah L. Elson ,  Kimberly Carr Ferguson ,  Sheila Akiko Homburger

发明人： Michael R. Costa ,  Stephen K. Doberstein ,  Sarah L. Elson ,  Kimberly Carr Ferguson ,  Sheila Akiko Homburger

IPC分类号： G01N3300

CPC分类号： C07K14/4705 ,  A01K67/0336 ,  A01K2217/05 ,  A01K2217/075 ,  C07K2319/00

摘要： Drosophila melanogaster and C. elegans that have been genetically modified to express or mis-express proteins involved in the sterol regulatory element binding protein (SREBP) pathway are described. These genetically modified animal models have identifiable phenotypes that make them useful in assays for studying lipid metabolism, other genes implicated in lipid metabolism, and compounds capable of modulating lipid metabolism pathways. Methods for studying lipid metabolism in living nematodes using fluorescently-labelled fatty acid conjugates, such BODITY™ fatty acid conjugates, are also described. Novel SREBP pathway nucleic acid and protein sequences are also described.

摘要翻译： 描述了已经被遗传修饰以表达或错误表达参与固醇调节元件结合蛋白（SREBP）途径的蛋白质的黑腹果蝇和秀丽隐杆线虫。 这些遗传修饰的动物模型具有可识别的表型，使其可用于研究脂质代谢，其他涉及脂质代谢的基因和能够调节脂质代谢途径的化合物。 还描述了使用荧光标记的脂肪酸共轭物（例如BODITY TM脂肪酸共轭物）研究活体线虫脂质代谢的方法。 还描述了新的SREBP途径核酸和蛋白质序列。

公开(公告)号：US06627746B1

公开(公告)日：2003-09-30

申请号：US09084303

申请日：1998-05-26

申请人： Stephen Kohl Doberstein ,  Darren Mark Platt ,  Kimberly Carr Ferguson ,  Andrew Roy Buchman ,  Sheila Akiko Homburger

发明人： Stephen Kohl Doberstein ,  Darren Mark Platt ,  Kimberly Carr Ferguson ,  Andrew Roy Buchman ,  Sheila Akiko Homburger

IPC分类号： C07H2104

CPC分类号： C07K14/43545 ,  A61K38/00

摘要： The present invention relates to C. elegans insulin-like genes and methods for identifying insulin-like genes. The methods provide nucleotide sequences of C. elegans insulin-like genes, amino acid sequences of their encoded proteins, and derivatives (e.g., fragments) and analogs thereof. The invention further relates to fragments (and derivatives and analogs thereof) of insulin-like proteins which comprise one or more domains of an insulin-like protein. Antibodies to an insulin-like protein, and derivatives and analogs thereof, are provided. Methods of production of an insulin-like protein (e.g., by recombinant means), and derivatives and analogs thereof, are provided. Further, methods to identify the biological function of a C. elegans insulin-like gene are provided, including various methods for the functional modification (e.g., overexpression, underexpression, mutation, knock-out) of one or more genes simultaneously. Still further, methods to identify a C. elegans gene which modifies the function of, and/or functions in a downstream pathway from, an insulin-like gene are provided.

公开(公告)号：US06703491B1

公开(公告)日：2004-03-09

申请号：US09270767

申请日：1999-03-17

申请人： Sheila Akiko Homburger ,  Allen James Ebens, Jr. ,  Catherine Sue Erickson ,  Helen Louise Francis-Lang ,  Jonathan Scott Margolis ,  Bindu Priya Reddy ,  David Andrew Ruddy ,  Andrew Roy Buchman

发明人： Sheila Akiko Homburger ,  Allen James Ebens, Jr. ,  Catherine Sue Erickson ,  Helen Louise Francis-Lang ,  Jonathan Scott Margolis ,  Bindu Priya Reddy ,  David Andrew Ruddy ,  Andrew Roy Buchman

IPC分类号： C07H2104

CPC分类号： A01K67/0333 ,  A01K67/0336 ,  A01K67/0337 ,  A01K67/0339 ,  A01K2217/05 ,  A01K2217/075 ,  C07K14/70571

摘要： The present invention relates to Drosophila genes and methods for their use. The invention provides nucleotide sequences of Drosophila genes, amino acid sequences of the encoded proteins, and derivatives (e.g., fragments) and analogs thereof. The invention further relates to fragments (and derivatives and analogs thereof) of proteins which comprise one or more domains of a Drosophila protein. Antibodies to Drosophila proteins, and derivatives and analogs thereof, are also provided. Also provided herein are vectors and host cells comprising such nucleic acids. Methods of production of a Drosophila protein (e.g., by recombinant means), and derivatives and analogs thereof, are provided. Chimeric polypeptide molecules comprising polypeptides of the invention fused to heterologous polypeptide sequences are provided. Methods to identify the biological function of a Drosophila gene are provided, including various methods for the functional modification (e.g., overexpression, underexpression, mutation, knock-out) of one gene, or of two or more genes simultaneously. Methods to identify a Drosophila gene which modifies the function of, and/or functions in a downstream pathway from, another gene are provided. The invention further provides for use of Drosophila proteins as media additives or pesticides.

摘要翻译： 本发明涉及果蝇基因及其使用方法。 本发明提供了果蝇基因的核苷酸序列，编码的蛋白质的氨基酸序列及其衍生物（例如片段）及其类似物。 本发明还涉及包含果蝇蛋白的一个或多个结构域的蛋白质的片段（及其衍生物和类似物）。 还提供了针对果蝇蛋白的抗体及其衍生物和类似物。 本文还提供了包含这种核酸的载体和宿主细胞。 提供了果蝇蛋白的生产方法（例如通过重组方法）及其衍生物和类似物。 提供了包含与异源多肽序列融合的本发明多肽的嵌合多肽分子。 提供鉴定果蝇基因生物学功能的方法，包括一种基因或两种或多种基因同时进行功能修饰（如过表达，低表达，突变，敲除）的各种方法。 提供了鉴定另一基因的修饰下游途径的功能和/或功能的果蝇基因的方法。 本发明还提供了果蝇蛋白质作为媒介添加剂或杀虫剂的用途。