-
1.发明申请Deleted sequences in M. bovis BCG/M bovis or M. tuberculosis, method for detecting mycobacteria using these sequences and vaccines 有权牛分枝杆菌BCG / M牛分枝杆菌或结核分枝杆菌中的缺失序列，使用这些序列和疫苗检测分枝杆菌的方法公开(公告)号：US20050250120A1
公开(公告)日：2005-11-10
申请号：US10980194
申请日：2004-11-04
摘要： The subject of the present invention is the identification of nucleotide and peptide sequences which make it possible in particular to distinguish, in diagnostic terms, an immunization resulting from a BCG vaccination from an M. tuberculosis infection. The sequences in question are specific either to M. bovis BCG/M. bovis, or to M. tuberculosis. The subject of the present invention is also a method for detecting the sequences in question, a method for detecting antibodies generated by the products of expression of these sequences and the kits for carrying out these methods. Finally, the subject of the present invention is novel vaccines.
摘要翻译： 本发明的主题是核苷酸和肽序列的鉴定，这使得特别可以在诊断方面区分由结核分枝杆菌感染引起的BCG接种引起的免疫。 所述序列对于牛分枝杆菌BCG / M是特异性的。 牛，或结核分枝杆菌。 本发明的主题还是用于检测所述序列的方法，检测由这些序列的表达产物产生的抗体的方法和用于实施这些方法的试剂盒。 最后，本发明的主题是新型疫苗。
-
2.发明申请DELETED SEQUENCES IN M. BOVIS BCG/M. BOVIS OR M. TUBERCULOSIS, METHOD FOR DETECTING MYCOBACTERIA USING SAID SEQUENCES AND VACCINES 审中-公开M.BVVIS BCG / M中的删除序列。 BOVIS或M.TUBERCULOSIS，使用确定序列和疫苗检测MYCOBACTERIA的方法公开(公告)号：US20130164749A1
公开(公告)日：2013-06-27
申请号：US13693940
申请日：2012-12-04
IPC分类号： C12Q1/68
摘要： The invention concerns the isolation of nucleotide and peptide sequences in particular for differentiating, in diagnostic terms, an immunisation resulting from BCG vaccination of an infection by M. tuberculosis. Said sequences are either M. bovis BCG/M. bovis specific or M. tuberculosis specific. The invention also concerns a method for detecting said sequences, a method for detecting antibodies generated by the products expressing said sequences as well as kits for implementing said methods. Finally, the invention concerns novel vaccines.
摘要翻译： 本发明涉及分离核苷酸和肽序列，特别是用于在诊断方面鉴别由结核分枝杆菌感染引起的BCG接种引起的免疫。 所述序列是牛分枝杆菌BCG / M。 牛结核特异性或结核分枝杆菌特异性。 本发明还涉及一种用于检测所述序列的方法，用于检测表达所述序列的产物产生的抗体的方法以及用于实现所述方法的试剂盒。 最后，本发明涉及新型疫苗。
-
3.发明授权Method for detecting mycobacteria using deleted sequences in M. bovis BCG/M. bovis or tuberculosis 有权使用牛分枝杆菌BCG / M中缺失的序列检测分枝杆菌的方法。 牛或结核病公开(公告)号：US08357493B2
公开(公告)日：2013-01-22
申请号：US10980194
申请日：2004-11-04
摘要： The invention concerns the isolation of nucleotide and peptide sequences in particular for differentiating, in diagnostic terms, an immunization resulting from BCG vaccination of an infection by M. tuberculosis. Said sequences are either M. bovis BCG/M. bovis specific or M. tuberculosis specific. The invention also concerns a method for detecting said sequences, a method for detecting antibodies generated by the products expressing said sequences as well as kits for implementing said methods. Finally, the invention concerns novel vaccines.
摘要翻译： 本发明涉及分离核苷酸和肽序列，特别是用于在诊断方面鉴别由结核分枝杆菌感染引起的BCG接种引起的免疫。 所述序列是牛分枝杆菌BCG / M。 牛结核特异性或结核分枝杆菌特异性。 本发明还涉及一种用于检测所述序列的方法，用于检测表达所述序列的产物产生的抗体的方法以及用于实现所述方法的试剂盒。 最后，本发明涉及新型疫苗。
-
4.发明申请Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a bac-based DNA library. application to the detection of mycobacteria 失效使用基于bac的DNA文库从分枝杆菌的基因组分离感兴趣的多核苷酸的方法。 应用于检测分枝杆菌公开(公告)号：US20050250104A1
公开(公告)日：2005-11-10
申请号：US10802796
申请日：2004-03-18
CPC分类号： C12Q1/689 , C07K14/35 , C12N15/1072 , C12Q1/6809
摘要： The present invention is directed to a method for isolating a polynucleotide of interest that is present or is expressed in a genome of a first mycobacterium strain and that is absent or altered in a genome of a second mycobacterium strain which is different from the first mycobacterium strain using a bacterial artificial chromosome (BAC) vector. The invention further relates to a polynucleotide isolated by this method and recombinant BAC vector used in this method. In addition the present invention comprises method and kit for detecting the presence of a mycobacteria in a biological sample.
摘要翻译： 本发明涉及分离在第一分枝杆菌菌株的基因组中存在或表达的目的多核苷酸的方法，并且在与第一分枝杆菌菌株不同的第二分枝杆菌菌株的基因组中不存在或改变 使用细菌人造染色体（BAC）载体。 本发明还涉及通过该方法分离的多核苷酸和用于该方法的重组BAC载体。 此外，本发明包括用于检测生物样品中分枝杆菌存在的方法和试剂盒。
-
公开(公告)号：US06991904B2
公开(公告)日：2006-01-31
申请号：US10259678
申请日：2002-09-30
CPC分类号： C12Q1/689 , C07K14/35 , C12N15/1072 , C12Q1/6809
摘要： A method for isolating a polynucleotide of interest that is present in the genome of a first mycobacterium strain and/or is expressed by the first mycobacterium strain, where the polynucleotide of interest is also absent or altered in the genome of a second mycobacterium strain and/or is not expressed in the second mycobacterium. The method includes (a) contacting the genomic DNA of the first mycobacterium strain under hybridizing conditions with the DNA of a least one clone that belongs to a bacterial artificial chromosome (BAC) genomic DNA library of the second mycobacterium strain, and (b) isolating the polynucleotide of interest that does not form a hybrid with the DNA of the second mycobacterium strain. This invention further pertains to a Mycobacterium tuberculosis strain H37Rv genomic DNA library, as well as a Mycobacterium bovis BCG strain Pasteur genomic DNA library, and the recombinant BAC vectors that belong to those genomic DNA libraries. This invention also relates to mycobacterial nucleic acids, and methods and kits for using these nucleic acids to detect mycobacteria in a biological sample.
-
6.发明授权Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library, application to the detection of mycobacteria 有权使用基于BAC的DNA文库从分枝杆菌基因组分离感兴趣的多核苷酸的方法，用于检测分枝杆菌公开(公告)号：US07112663B1
公开(公告)日：2006-09-26
申请号：US09673476
申请日：1999-04-16
CPC分类号： C12Q1/689 , C07K14/35 , C12N15/1072 , C12Q1/6809
摘要： A method for isolating a polynucleotide of interest that is present in the genome of a first mycobacterium strain and/or is expressed by the first mycobacterium strain, where the polynucleotide of interest is also absent or altered in the genome of a second mycobacterium strain and/or is not expressed in the second mycobacterium. The method includes (a) contacting the genomic DNA of the first mycobacterium strain under hybridizing conditions with the DNA of a least one clone that belongs to a bacterial artificial chromosome (BAC) genomic DNA library of the second mycobacterium strain, and (b) isolating the polynucleotide of interest that does not form a hybrid with the DNA of the second mycobacterium strain. This invention further pertains to a Mycobacterium tuberculosis strain H37Rv genomic DNA library, as well as a Mycobacterium bovis BCG strain Pasteur genomic DNA library, and the recombinant BAC vectors that belong to those genomic DNA libraries. This invention also relates to mycobacterial nucleic acids, and methods and kits for using these nucleic acids to detect mycobacteria in a biological sample.
摘要翻译： 用于分离存在于第一分枝杆菌菌株的基因组中和/或由第一分枝杆菌菌株表达的目标多核苷酸的方法，其中感兴趣的多核苷酸在第二分枝杆菌菌株的基因组中也不存在或改变，和/ 或不在第二分枝杆菌中表达。 该方法包括（a）在杂交条件下将第一分枝杆菌菌株的基因组DNA与属于第二分枝杆菌菌株的细菌人造染色体（BAC）基因组DNA文库的至少一个克隆的DNA接触，和（b）分离 不与第二分枝杆菌菌株的DNA形成杂交的感兴趣的多核苷酸。 本发明还涉及结核分枝杆菌菌株H37Rv基因组DNA文库以及牛分枝杆菌BCG菌株巴斯德基因组DNA文库和属于这些基因组DNA文库的重组BAC载体。 本发明还涉及分枝杆菌核酸，以及使用这些核酸来检测生物样品中的分枝杆菌的方法和试剂盒。
-
7.发明申请Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library: application to the detection of mycobacteria 审中-公开使用基于BAC的DNA文库从分枝杆菌基因组分离感兴趣的多核苷酸的方法：应用于检测分枝杆菌公开(公告)号：US20060063182A1
公开(公告)日：2006-03-23
申请号：US11221284
申请日：2005-09-08
CPC分类号： C12Q1/689 , C07K14/35 , C12N15/1072 , C12Q1/6809
摘要： A method for isolating a polynucleotide of interest that is present in the genome of and/or expressed by a first mycobacterium strain that is also absent or altered in the genome of and/or not expressed by a second mycobacterium strain. The method includes contacting the genomic DNA of the first strain under hybridizing conditions with the DNA of at least one clone belonging to a bacterial artificial chromosome (BAC) genomic DNA library of the second strain and isolating the polynucleotide of interest that does not hybridize with the DNA of the second strain. This invention further pertains to a Mycobacterium tuberculosis strain H37Rv genomic DNA library, a Mycobacterium bovis BCG strain Pasteur genomic DNA library, and the recombinant BAC vectors belonging to those genomic DNA libraries. This invention also relates to mycobacterial nucleic acids, and methods and kits for using these nucleic acids to detect mycobacteria in a biological sample.
摘要翻译： 用于分离存在于第一分枝杆菌菌株的基因组中和/或由第二分枝杆菌菌株在基因组中不存在或改变并且/或不由第二分枝杆菌菌株表达的第一分枝杆菌菌株表达的目的多核苷酸的方法。 该方法包括将杂交条件下的第一菌株的基因组DNA与属于第二菌株的细菌人造染色体（BAC）基因组DNA文库的至少一个克隆的DNA接触，并分离不与第二菌株杂交的目的多核苷酸 第二菌株的DNA。 本发明还涉及结核分枝杆菌菌株H37Rv基因组DNA文库，牛分枝杆菌BCG菌株巴斯德基因组DNA文库和属于这些基因组DNA文库的重组BAC载体。 本发明还涉及分枝杆菌核酸，以及使用这些核酸来检测生物样品中的分枝杆菌的方法和试剂盒。
-
8.发明授权Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library: application to the detection of mycobacteria 有权使用基于BAC的DNA文库从分枝杆菌基因组分离感兴趣的多核苷酸的方法：应用于检测分枝杆菌公开(公告)号：US08455187B2
公开(公告)日：2013-06-04
申请号：US11341914
申请日：2006-01-30
CPC分类号： C12Q1/689 , C07K14/35 , C12N15/1072 , C12Q1/6809
摘要： A method for detecting Mycobacterium tuberculosis involves contacting a biological sample with the product of expression of at least one ORF contained in a polynucleotide present in Mycobacterium tuberculosis and absent in Mycabacterium bovis BCG; and detecting whether an immunological complex is formed between the product thereof and antibodies contained in the biological sample, wherein the presence of a complex indicates an infection with Mycobacterium tuberculosis.
摘要翻译： 用于检测结核分枝杆菌的方法包括使生物样品与存在于结核分枝杆菌中存在的多核苷酸中含有的至少一个ORF的表达产物接触，并且在牛分枝杆菌BCG中不存在; 并检测其产物与生物样品中所含的抗体之间是否形成免疫复合物，其中复合物的存在表示结核分枝杆菌感染。
-
9.发明授权Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library. application to the detection of mycobacteria 失效使用基于BAC的DNA文库从分枝杆菌的基因组分离感兴趣的多核苷酸的方法。 应用于检测分枝杆菌公开(公告)号：US07842781B2
公开(公告)日：2010-11-30
申请号：US10802796
申请日：2004-03-18
CPC分类号： C12Q1/689 , C07K14/35 , C12N15/1072 , C12Q1/6809
摘要： The present invention is directed to a method for isolating a polynucleotide of interest that is present or is expressed in a genome of a first mycobacterium strain and that is absent or altered in a genome of a second mycobacterium strain which is different from the first mycobacterium strain using a bacterial artificial chromosome (BAC) vector. The invention further relates to a polynucleotide isolated by this method and recombinant BAC vector used in this method. In addition the present invention comprises method and kit for detecting the presence of a mycobacteria in a biological sample.
摘要翻译： 本发明涉及分离在第一分枝杆菌菌株的基因组中存在或表达的目的多核苷酸的方法，并且在与第一分枝杆菌菌株不同的第二分枝杆菌菌株的基因组中不存在或改变 使用细菌人造染色体（BAC）载体。 本发明还涉及通过该方法分离的多核苷酸和用于该方法的重组BAC载体。 此外，本发明包括用于检测生物样品中分枝杆菌存在的方法和试剂盒。
-
10.发明申请Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library: application to the detection of mycobacteria 有权使用基于BAC的DNA文库从分枝杆菌基因组分离感兴趣的多核苷酸的方法：应用于检测分枝杆菌公开(公告)号：US20070092884A1
公开(公告)日：2007-04-26
申请号：US11341914
申请日：2006-01-30
CPC分类号： C12Q1/689 , C07K14/35 , C12N15/1072 , C12Q1/6809
摘要： A method for detecting Mycobacterium tuberculosis involves contacting a biological sample with the product of expression of at least one ORF contained in a polynucleotide present in Mycobacterium tuberculosis and absent in Mycabacterium bovis BCG; and detecting whether an immunological complex is formed between the product thereof and antibodies contained in the biological sample, wherein the presence of a complex indicates an infection with Mycobacterium tuberculosis.
摘要翻译： 用于检测结核分枝杆菌的方法包括使生物样品与存在于结核分枝杆菌中存在的多核苷酸中含有的至少一个ORF的表达产物接触，并且在牛分枝杆菌BCG中不存在; 并检测其产物与生物样品中所含的抗体之间是否形成免疫复合物，其中复合物的存在表示结核分枝杆菌感染。
-
-
-
-
-
-
-
-
-
搜索结果
12 条记录 (耗时 0.037 秒)
