公开(公告)号：US5559003A

公开(公告)日：1996-09-24

申请号：US348870

申请日：1994-11-29

申请人： Sumi Kawahara ,  Toshikatsu Abe ,  Kenji Hosoi

发明人： Sumi Kawahara ,  Toshikatsu Abe ,  Kenji Hosoi

IPC分类号： C12Q1/28 ,  C12Q1/26 ,  C12Q1/54 ,  G01N33/48

CPC分类号： C12Q1/28 ,  C12Q2326/96 ,  Y10T436/103332 ,  Y10T436/104165 ,  Y10T436/104998

摘要： The method is directed to assaying for biological components in a sample comprising step (A) generating an oxidase substrate in the presence of an amphoteric surfactant and in the absence of ferrocyanide; (B) initially generating hydrogen peroxide through said oxidase reaction on said substrate of oxidase with subsequent detection of the generated hydrogen peroxide using peroxidase and a color developer capable of being oxidized in the presence of amphoteric surfactant and ferrocyanide; and (C) correlating the amount of color developed to the amount of biological components in the biological sample. Even when the biological component to be detected is present in a very small amount, the interference of bilirubin can be eliminated in assays of biological components in which peroxidase generated from an enzymatic reaction is detected using peroxidase and a color developer capable of being oxidized.

摘要翻译： 该方法用于测定包含在两性表面活性剂存在下和在不存在亚铁氰化物的情况下产生氧化酶底物的步骤（A）的样品中的生物组分; （B）最初通过所述氧化酶反应在所述氧化酶底物上产生过氧化氢，随后使用过氧化物酶检测所产生的过氧化氢和能够在两性表面活性剂和亚铁氰化物存在下被氧化的显色剂; 和（C）将生成的生物成分的颜色量与生物样品的生成成分的量相关联。 即使要检测的生物成分以非常小的量存在，也可以在使用过氧化物酶和能够被氧化的彩色显影剂检测由酶反应产生的过氧化物酶的生物成分的测定中，可以消除胆红素的干扰。