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    Recombinant HIV and modified packaging cells and method for treating acquired immune deficiency syndrome
    3.
    发明授权
    Recombinant HIV and modified packaging cells and method for treating acquired immune deficiency syndrome 失效
    重组HIV和改良包装细胞及治疗获得性免疫缺陷综合征的方法

    公开(公告)号：US5753490A

    公开(公告)日：1998-05-19

    申请号：US563597

    申请日：1995-11-28

    申请人： Syed Zaki Salahuddin ,  Nickolas Chelyapov

    发明人： Syed Zaki Salahuddin ,  Nickolas Chelyapov

    IPC分类号： C12N7/00 ,  C12N15/867 ,  C12N7/01 ,  C12N7/02 ,  C12N7/04

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2740/16021 ,  C12N2740/16043

    摘要： A recombinant human immunodeficiency virus (rHIV) and recombinant mammalian cell-line (packaging systems) and a method for treating infection of cells by human immuneodeficiency virus HIV. rHIV, such as rHIV-1, comprises of a gene construction which includes a foreign gene. The expression of this gene is activated in human cells in the presence of wild-type HIV. This gene product can cause cell death in the presence of an appropriate drug, e.g. Acyclovir. This gene product is typically a viral thymidine kinase. rHIV is so constructed that it is unable to replicate by itself due to the absence of a regulatory gene that is necessary for its replication, such as tat or rev or both. The recombinant mammalian cell-line packaging system comprises in its genome a recombinant gene construction which typically includes a functional regulatory gene from HIV which is missing from rHIV, such as the tat or rev genes or both. The method for treating infection of cells by HIV comprises administering a composition comprising rHIV, which is produced in vitro from a recombinant mammalian cell-line, and treating with a nucleoside analog, such as Acyclovir or Gancyclovir.

    摘要翻译： 重组人类免疫缺陷病毒（rHIV）和重组哺乳动物细胞系（包装系统）以及人免疫缺陷病毒HIV感染细胞的方法。 rHIV，如rHIV-1，由包含外来基因的基因构建构成。 在野生型HIV存在下，该基因的表达在人细胞中被激活。 该基因产物可以在合适的药物存在下引起细胞死亡，例如， 阿昔洛韦 该基因产物通常是病毒胸苷激酶。 由于缺乏对其复制所必需的调节基因，例如tat或rev或两者，rHIV是如此构建，使得它不能自己复制。 重组哺乳动物细胞系包装系统在其基因组中包含重组基因构建体，其通常包含来自HIV的功能调节基因，其从rHIV缺失，例如tat或rev基因或两者。 用于治疗HIV感染细胞的方法包括施用包含rHIV的组合物，所述组合物在体外从重组哺乳动物细胞系产生，并用核苷类似物如阿昔洛韦或Gancyclovir进行治疗。

    Recombinant HIV and modified packaging cells and method for using
    4.
    发明授权
    Recombinant HIV and modified packaging cells and method for using 失效
    重组HIV和改良包装细胞及其使用方法

    公开(公告)号：US6063374A

    公开(公告)日：2000-05-16

    申请号：US563360

    申请日：1995-11-28

    申请人： Syed Zaki Salahuddin ,  Nickolas Chelyapov

    发明人： Syed Zaki Salahuddin ,  Nickolas Chelyapov

    IPC分类号： C12N7/00 ,  C12N15/867 ,  A61K48/00 ,  C12N15/00 ,  C12N15/85

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2740/16021 ,  C12N2740/16043

    摘要： A recombinant human immunodeficiency virus (rHIV) and recombinant mammalian cell-line (packaging systems) and a method for treating infection of cells by human immuneodeficiency virus HIV. rHIV, such as rHIV-1, comprises of a gene construction which includes a foreign gene. The expression of this gene is activated in human cells in the presence of wild-type HIV. This gene product can cause cell death in the presence of an appropriate drug, e.g. Acyclovir. This gene product is typically a viral thymidine kinase. rHIV is so constructed that it is unable to replicate by itself due to the absence of a regulatory gene that is necessary for its replication, such as tat or rev or both. The recombinant mammalian cell-line packaging system comprises in its genome a recombinant gene construction which typically includes a functional regulatory gene from HIV which is missing from rHIV, such as the tat or rev genes or both. The method for treating infection of cells by HIV comprises administering a composition comprising rHIV, which is produced in vitro from a recombinant mammalian cell-line, and treating with a nucleoside analog, such as Acyclovir or Gancyclovir.

    摘要翻译： 重组人类免疫缺陷病毒（rHIV）和重组哺乳动物细胞系（包装系统）以及人免疫缺陷病毒HIV感染细胞的方法。 rHIV，如rHIV-1，由包含外来基因的基因构建构成。 在野生型HIV存在下，该基因的表达在人细胞中被激活。 该基因产物可以在合适的药物存在下引起细胞死亡，例如， 阿昔洛韦 该基因产物通常是病毒胸苷激酶。 由于缺乏对其复制所必需的调节基因，例如tat或rev或两者，rHIV是如此构建，使得它不能自己复制。 重组哺乳动物细胞系包装系统在其基因组中包含重组基因构建体，其通常包含来自HIV的功能调节基因，其从rHIV缺失，例如tat或rev基因或两者。 用于治疗HIV感染细胞的方法包括施用包含rHIV的组合物，所述组合物在体外从重组哺乳动物细胞系产生，并用核苷类似物如阿昔洛韦或Gancyclovir进行治疗。