公开(公告)号：US09850498B2

公开(公告)日：2017-12-26

申请号：US14649317

申请日：2013-12-10

申请人： TAKARA BIO INC.

发明人： Katsuyuki Dodo ,  Takahisa Tsukihara ,  Mayumi Shimomura ,  Koichi Inoue ,  Hideto Chono ,  Junichi Mineno ,  Masanari Kitagawa

IPC分类号： C12P21/02 ,  C12N15/63 ,  C12N15/85 ,  C07H21/00 ,  C07H21/04 ,  C12N15/09 ,  C12N15/113

CPC分类号： C12N15/85 ,  C12N15/63 ,  C12N2740/16311

摘要： The present invention provides an expression cassette comprising (1) an expression regulatory region having a sequence corresponding to a transcriptional activator-binding region and (2) a nucleic acid encoding a transcriptional activator which is capable of binding to the expression regulatory region, wherein the nucleic acid is operably linked to the expression regulatory region; a method for expressing a target product in a mammalian cell, a method for producing a cell expressing a target product, and a method for producing a target product in a mammalian cell using the expression cassette; and a kit comprising the expression cassette.