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公开(公告)号：US11999972B2
公开(公告)日：2024-06-04
申请号：US16321112
申请日：2017-07-28
申请人： TAKARA BIO INC.
发明人： Tomomi Otsuji , Toshikazu Nishie , Risa Kato , Sachiko Okamoto , Tatsuji Enoki , Junichi Mineno
CPC分类号： C12N5/0696 , A61K38/39 , C07K14/78 , C12M25/06 , C12M23/20 , C12N2533/52
摘要： Stem cells can be efficiently proliferated by culturing the stem cells in the presence of a novel recombinant fibronectin fragment.
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公开(公告)号：US09938541B2
公开(公告)日：2018-04-10
申请号：US14649603
申请日：2013-12-20
申请人： TAKARA BIO INC.
IPC分类号： C07H21/04 , C12N15/86 , C12N15/00 , C12N7/00 , C07K14/005
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , C12N2750/14122 , C12N2750/14142 , C12N2750/14143
摘要： The invention provides an AAV particle containing an adeno-associated viral (AAV) capsid protein having an amino acid sequence selected from the group consisting of SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 24, and SEQ ID NO: 30 of the sequence listing; a nucleic acid that encodes this capsid protein; DNA containing this nucleic acid; a cell containing this DNA; and a method for producing this cell.
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公开(公告)号：US09850498B2
公开(公告)日：2017-12-26
申请号：US14649317
申请日：2013-12-10
申请人： TAKARA BIO INC.
发明人： Katsuyuki Dodo , Takahisa Tsukihara , Mayumi Shimomura , Koichi Inoue , Hideto Chono , Junichi Mineno , Masanari Kitagawa
CPC分类号： C12N15/85 , C12N15/63 , C12N2740/16311
摘要： The present invention provides an expression cassette comprising (1) an expression regulatory region having a sequence corresponding to a transcriptional activator-binding region and (2) a nucleic acid encoding a transcriptional activator which is capable of binding to the expression regulatory region, wherein the nucleic acid is operably linked to the expression regulatory region; a method for expressing a target product in a mammalian cell, a method for producing a cell expressing a target product, and a method for producing a target product in a mammalian cell using the expression cassette; and a kit comprising the expression cassette.
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公开(公告)号：US09603874B2
公开(公告)日：2017-03-28
申请号：US14870596
申请日：2015-09-30
申请人： MIE UNIVERSITY , TAKARA BIO INC.
发明人： Hiroshi Shiku , Hiroaki Ikeda , Koichi Iwamura , Junichi Mineno , Ikunoshin Kato
IPC分类号： C12N15/11 , A61K35/17 , C12N5/0783 , C12N15/113 , A61K35/12 , A61K39/00
CPC分类号： A61K35/17 , A61K2035/124 , A61K2039/5158 , C12N5/0636 , C12N15/113 , C12N2310/14 , C12N2501/48 , C12N2501/599 , C12N2310/531
摘要： Disclosed is a method for enhancing the function of a T cell, which is characterized by inhibiting the expression of programmed death-1 ligand 1 (PD-L1) and/or programmed death-1 ligand 2 (PD-L2) in the T cell. Also disclosed is a function-enhanced T cell which is produced by the function enhancement method. Further disclosed is a therapeutic agent comprising the function-enhanced T cell. The T cell can enhance an immune response to cancer, and is useful in an immunotherapy effective for cancer and the treatment or prevention of infectious diseases and autoimmune diseases.
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公开(公告)号：US09399780B2
公开(公告)日：2016-07-26
申请号：US14381721
申请日：2013-03-14
申请人： TAKARA BIO INC.
CPC分类号： C12N15/86 , A61K35/13 , A61K48/00 , C12N5/0018 , C12N7/00 , C12N2740/10041 , C12N2740/10051 , C12N2740/10052
摘要： The present invention provides a method for producing a virus vector, which involves a step of culturing a cell capable of producing the virus vector in a culture medium containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate; and a culture medium for use in the production of a virus vector, which is characterized by containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate as active ingredients.
摘要翻译： 本发明提供一种病毒载体的制备方法，其包括在含有维甲酸化合物，组蛋白脱乙酰酶抑制物质和能够形成螯合物的培养基中培养能够产生病毒载体的细胞的步骤 ; 以及用于生产病毒载体的培养基，其特征在于含有视黄酸化合物，组蛋白脱乙酰酶抑制物质和能够形成螯合物的物质作为活性成分。
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公开(公告)号：US09296807B2
公开(公告)日：2016-03-29
申请号：US14701929
申请日：2015-05-01
申请人： TAKARA BIO INC. , MIE UNIVERSITY
发明人： Junichi Mineno , Sachiko Okamoto , Risa Sumioka , Masanari Kitagawa , Hiroshi Shiku , Ikunoshin Kato
IPC分类号： C12P21/06 , C12N5/00 , C12N15/63 , C07K14/725 , C12N15/85
CPC分类号： C07K14/7051 , C12N15/85 , C12N2800/40 , C12N2840/102
摘要： Disclosed is a cell which can express a non-natural oligomeric protein, which has, introduced therein, a gene encoding an exogenous polypeptide corresponding to at least one endogenous polypeptide constituting a natural oligomeric protein, and in which the expression of the endogenous polypeptide is inhibited.
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公开(公告)号：US09228206B2
公开(公告)日：2016-01-05
申请号：US14457453
申请日：2014-08-12
申请人： TAKARA BIO INC.
发明人： Katsuyuki Dodo , Naoki Saito , Hideto Chono , Junichi Mineno
CPC分类号： C12N15/86 , C12N2740/10043
摘要： Disclosed is a simple and highly efficient method for introducing a gene into a target cell using a retrovirus vector. The method comprises the steps of (a) placing a liquor containing a retrovirus vector having a foreign gene carried thereon into a bag for cell culture on which a retrovirus-binding substance has been immobilized, and incubating the liquor at a temperature lower than 25° C. for 8-48 hours, thereby producing a culture bag having the retrovirus vector bound thereto, (b) adding a target cell to the culture bag that has been produced in step (a) and incubating the culture bag for 8 hours or less, and (c) flipping the culture bag upside down and incubating the culture bag. The gene introduction method is useful particularly in medicine, cell technology, gene technology, and embryologic technology.
摘要翻译： 公开了使用逆转录病毒载体将基因导入靶细胞的简单且高效的方法。 该方法包括以下步骤：（a）将含有携带有外来基因的逆转录病毒载体的液体放入已经固定有逆转录病毒结合物质的细胞培养袋中，并在低于25℃的温度下培养该液体 制备具有结合反转录病毒载体的培养袋，（b）向步骤（a）中生产的培养袋中加入靶细胞，并将培养袋孵育8小时以下 ，（c）颠倒培养袋并培养培养袋。 基因导入方法特别适用于医学，细胞技术，基因技术和胚胎学技术。
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公开(公告)号：US20150291980A1
公开(公告)日：2015-10-15
申请号：US14409134
申请日：2013-06-26
申请人： TAKARA BIO INC.
CPC分类号： C12N15/86 , C12N5/10 , C12N7/00 , C12N15/09 , C12N15/111 , C12N15/64 , C12N2310/141 , C12N2330/51 , C12N2501/65 , C12N2750/14143 , C12N2750/14151 , C12P19/34
摘要： According to the present invention, an AAV vector having a higher titer compared with those of conventional ones can be produced using a cell into which a nucleic acid capable of expressing miRNA is introduced artificially. An AAV vector produced using the cell and a composition containing the viral vector as an active ingredient are very useful as gene transfer means in the studies or clinical practice of gene therapies.
摘要翻译： 根据本发明，可以使用人工引入能够表达miRNA的核酸的细胞来生产具有比常规滴定度更高的效价的AAV载体。 使用细胞产生的AAV载体和含有病毒载体作为活性成分的组合物在基因治疗的研究或临床实践中作为基因转移手段非常有用。
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公开(公告)号：US12018276B2
公开(公告)日：2024-06-25
申请号：US16961773
申请日：2019-01-24
申请人： TAKARA BIO INC.
发明人： Tomomi Otsuji , Yuka Hirase , Asako Hatsuyama , Sachiko Okamoto , Tatsuji Enoki , Junichi Mineno
CPC分类号： C12N5/0068 , C07K14/78 , C12N5/0634 , C12N2533/50 , C12N2533/52
摘要： In the present invention, lymphocytes are efficiently grown by culturing lymphocytes in the presence of a novel recombinant fibronectin fragment.
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公开(公告)号：US09175308B2
公开(公告)日：2015-11-03
申请号：US14349763
申请日：2012-10-05
申请人： MIE UNIVERSITY , TAKARA BIO INC.
发明人： Hiroshi Shiku , Yuki Orito , Junichi Mineno , Sachiko Okamoto , Yasunori Amaishi
IPC分类号： C12N5/10 , C12N5/0783 , C07H21/04 , C07K14/705 , C07K19/00 , C12N15/85 , C07K14/725 , C07K16/30
CPC分类号： C12N15/85 , C07K14/7051 , C07K14/70517 , C07K14/70521 , C07K14/70578 , C07K14/70596 , C07K16/30 , C07K2319/00 , C07K2319/03 , C07K2319/30
摘要： Provided are a chimeric antigen receptor comprising an extracellular domain capable of binding to an antigen, a transmembrane domain and at least one intracellular domain, the chimeric antigen receptor being characterized in that an intracellular domain of a glucocorticoid-induced tumor necrosis factor receptor (GITR) is contained as the intracellular domain; a nucleic acid encoding the chimeric antigen receptor; a cell expressing the chimeric antigen receptor; and a method for producing the cell.
摘要翻译： 提供了包含能够结合抗原，跨膜结构域和至少一个细胞内结构域的细胞外结构域的嵌合抗原受体，所述嵌合抗原受体的特征在于糖皮质激素诱导的肿瘤坏死因子受体（GITR）的细胞内结构域， 作为细胞内结构域; 编码嵌合抗原受体的核酸; 表达嵌合抗原受体的细胞; 以及该细胞的制造方法。
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