公开(公告)号：US09228206B2

公开(公告)日：2016-01-05

申请号：US14457453

申请日：2014-08-12

申请人： TAKARA BIO INC.

发明人： Katsuyuki Dodo ,  Naoki Saito ,  Hideto Chono ,  Junichi Mineno

IPC分类号： C12N15/00 ,  C12N15/86 ,  C12N15/87 ,  C12P21/04 ,  C12P21/06

CPC分类号： C12N15/86 ,  C12N2740/10043

摘要： Disclosed is a simple and highly efficient method for introducing a gene into a target cell using a retrovirus vector. The method comprises the steps of (a) placing a liquor containing a retrovirus vector having a foreign gene carried thereon into a bag for cell culture on which a retrovirus-binding substance has been immobilized, and incubating the liquor at a temperature lower than 25° C. for 8-48 hours, thereby producing a culture bag having the retrovirus vector bound thereto, (b) adding a target cell to the culture bag that has been produced in step (a) and incubating the culture bag for 8 hours or less, and (c) flipping the culture bag upside down and incubating the culture bag. The gene introduction method is useful particularly in medicine, cell technology, gene technology, and embryologic technology.

摘要翻译： 公开了使用逆转录病毒载体将基因导入靶细胞的简单且高效的方法。 该方法包括以下步骤：（a）将含有携带有外来基因的逆转录病毒载体的液体放入已经固定有逆转录病毒结合物质的细胞培养袋中，并在低于25℃的温度下培养该液体 制备具有结合反转录病毒载体的培养袋，（b）向步骤（a）中生产的培养袋中加入靶细胞，并将培养袋孵育8小时以下 ，（c）颠倒培养袋并培养培养袋。 基因导入方法特别适用于医学，细胞技术，基因技术和胚胎学技术。