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公开(公告)号：US20230242897A1
公开(公告)日：2023-08-03
申请号：US18298742
申请日：2023-04-11
Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED
Inventor： Meinhard Hasslacher , Christian Fiedler , Christa Mayer , Artur Mitterer
CPC classification number: C12N9/6489 , C12Y304/24087 , C12N11/14 , C12M47/12
Abstract: Provided herein are methods for purifying recombinant A Disintegrin-like and Metallopeptidase with Thrombospondin Type 1 Motif 13 (ADAMTS13) protein from a sample. The method comprises enriching for ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS13 protein to appear in the eluate or supernatant from the hydroxylapatite. The methods may further comprise tandem chromatography with a mixed mode cation exchange/hydrophobic interaction resin that binds ADAMTS13 protein. Additional optional steps involve ultrafiltration/diafiltration, anion exchange chromatography, cation exchange chromatography, and viral inactivation. Also provided herein are methods for inactivating virus contaminants in protein samples, where the protein is immobilized on a support. Also provided herein are compositions of ADAMTS13 prepared according to said methods.
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公开(公告)号：US11421202B2
公开(公告)日：2022-08-23
申请号：US16816641
申请日：2020-03-12
Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED
Inventor： Wolfgang Mundt , Artur Mitterer , Manfred Reiter , Meinhard Hasslacher , Leopold Grillberger , Thomas Kreil
Abstract: The invention relates to a method for removing a viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. The method comprises subjecting said preparation to filtration for at least about 24 hours through a virus filter having an effective pore size of maximum about 75 nm. Further, the invention relates to the use of a virus filter in filtration of at least about 24 hours, wherein the virus filter has an effective pore size of maximum about 75 nm for the removal of viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. In some embodiments the filtration according to the invention operates at a volumetric capacity of at least about 2000 L/m2. Further, the invention relates to the use of a preparation, being a cell culture medium or at least a component of a cell culture medium obtainable according to method of the invention for cell culture; pharmaceutical, diagnostic and/or cosmetic preparations as well as in food preparations.
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公开(公告)号：US11661593B2
公开(公告)日：2023-05-30
申请号：US14571670
申请日：2014-12-16
Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED
Inventor： Meinhard Hasslacher , Christian Fiedler , Christa Mayer , Artur Mitterer
CPC classification number: C12N9/6489 , C12M47/12 , C12N11/14 , C12Y304/24087
Abstract: Provided herein are methods for purifying recombinant A Disintegrin-like and Metallopeptidase with Thrombospondin Type 1 Motif 13 (ADAMTS13) protein from a sample. The method comprises enriching for ADAMTS13 protein by chromatographically contacting the sample with hydroxyapatite under conditions that allow ADAMTS13 protein to appear in the eluate or supernatant from the hydroxylapatite. The methods may further comprise tandem chromatography with a mixed mode cation exchange/hydrophobic interaction resin that binds ADAMTS13 protein. Additional optional steps involve ultrafiltration/diafiltration, anion exchange chromatography, cation exchange chromatography, and viral inactivation. Also provided herein are methods for inactivating virus contaminants in protein samples, where the protein is immobilized on a support. Also provided herein are compositions of ADAMTS13 prepared according to said methods.
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