公开(公告)号：US11421202B2

公开(公告)日：2022-08-23

申请号：US16816641

申请日：2020-03-12

Applicant: TAKEDA PHARMACEUTICAL COMPANY LIMITED

Inventor： Wolfgang Mundt ,  Artur Mitterer ,  Manfred Reiter ,  Meinhard Hasslacher ,  Leopold Grillberger ,  Thomas Kreil

IPC: C12N5/071 ,  A61L2/00 ,  C12M1/00 ,  C12M1/12

Abstract: The invention relates to a method for removing a viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. The method comprises subjecting said preparation to filtration for at least about 24 hours through a virus filter having an effective pore size of maximum about 75 nm. Further, the invention relates to the use of a virus filter in filtration of at least about 24 hours, wherein the virus filter has an effective pore size of maximum about 75 nm for the removal of viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. In some embodiments the filtration according to the invention operates at a volumetric capacity of at least about 2000 L/m2. Further, the invention relates to the use of a preparation, being a cell culture medium or at least a component of a cell culture medium obtainable according to method of the invention for cell culture; pharmaceutical, diagnostic and/or cosmetic preparations as well as in food preparations.

公开(公告)号：US11780904B2

公开(公告)日：2023-10-10

申请号：US17086072

申请日：2020-10-30

Applicant: Takeda Pharmaceutical Company Limited

Inventor： Leopold Grillberger ,  Manfred Reiter ,  Wolfgang Mundt

IPC: C07K14/755 ,  C12N9/64 ,  A61K9/00 ,  A61K38/36 ,  A61K38/37 ,  C12P21/00 ,  A61K38/00

CPC classification number: C07K14/755 ,  A61K9/0019 ,  A61K38/36 ,  A61K38/37 ,  C12N9/6489 ,  C12P21/00 ,  A61K38/00 ,  C12N2500/24 ,  C12N2511/00 ,  C12Y304/24087

Abstract: Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH4+) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.