公开(公告)号：US20190195701A1

公开(公告)日：2019-06-27

申请号：US16092614

申请日：2017-04-13

申请人： TECHNISCHE UNIVERSITEIT DELFT

发明人： Kourosh HONARMAND EBRAHIMI ,  Morten VAN SCHIE ,  Peter Leon HAGEDOORN ,  Wilfred Raymond HAGEN

IPC分类号： G01K17/00 ,  G01N25/48 ,  G01K3/08 ,  G01N21/05

CPC分类号： G01K17/006 ,  G01K3/08 ,  G01K19/00 ,  G01N21/05 ,  G01N25/482 ,  G01N25/4873 ,  G01N2021/0346

摘要： The invention provides a calibration method for calibrating a chip-based microfluidic calorimeter, wherein the chip-based microfluidic calorimeter comprises one or more thermopiles, wherein the calibration method uses the deprotonating reaction of a phosphate group, the method comprising: providing calibration liquids comprising (i) a buffer with a pH range of at least 7-9 and (ii) a first compound with a phosphate group which is protonated in a pH range of at least 3-6, and mixing these calibration liquids in the chip-based microfluidic calorimeter to provide a calibration liquid mixture whereby heat is generated, measuring the heat by the thermopiles and thereby providing a corresponding thermopile signal, and calibrating the chip-based microfluidic calorimeter by relating the thermopile signal to reference data of the deprotonating reaction.