公开(公告)号：US20180371468A1

公开(公告)日：2018-12-27

申请号：US15737696

申请日：2017-02-21

Applicant: Teknologian tutkimuskeskus VTT Oy

Inventor： Dominik MOJZITA ,  Anssi RANTASALO ,  Jussi JÄNTTI ,  Christopher LANDOWSKI ,  Joosu KUIVANEN

IPC: C12N15/79 ,  C12N15/62

Abstract: The present invention provides an expression system for a eukaryotic host, which comprises 1) an expression cassette comprising a core promoter, said core promoter controlling the expression of a DNA sequence encoding a synthetic transcription factor (sTF), and 2) one or more expression cassettes each comprising a DNA sequence encoding a desired product operably linked to a synthetic promoter, said synthetic promoter comprising a core promoter, and sTF-specific binding sites upstream of the core promoter. The present invention also provides a method for identifying universal core promoters for eukaryotic hosts, expression systems using universal core promoters, hosts comprising said systems, and methods for producing protein products in eukaryotic hosts.

公开(公告)号：US20230111619A1

公开(公告)日：2023-04-13

申请号：US17777885

申请日：2020-11-18

Applicant: Teknologian tutkimuskeskus VTT Oy

Inventor： Dominik MOJZITA ,  Outi KOIVISTOINEN ,  Astrid SALUMÄE

IPC: C07K14/415 ,  C12N15/80 ,  C12N15/81

Abstract: The present invention relates to the fields of life sciences, genetics and regulation of gene expression. Specifically, the invention relates to a non-viral transcription activation domain for a eukaryotic host. Also, the present invention relates to a polypeptide or artificial transcription factor comprising the transcription activation domain of the present invention. And furthermore, the present invention relates to a polynucleotide, an expression cassette, expression system, and/or a eukaryotic host. Still, the present invention relates to a method for producing a desired protein product in the eukaryotic host of the present invention or to a method of preparing a non-viral transcription activation domain of the present invention or a polynucleotide encoding said non-viral transcription activation domain. And still further, the present invention relates to use of the transcription activation domain, polypeptide, artificial transcription factor, polynucleotide, expression cassette, expression system or eukaryotic host of the present invention for metabolic engineering and/or production of a desired protein product.

公开(公告)号：US20210010015A1

公开(公告)日：2021-01-14

申请号：US16980710

申请日：2019-03-11

Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTT OY

Inventor： Dominik MOJZITA ,  Heiko RISCHER ,  Anssi RANTASALO ,  Kirsi-Marja OKSMAN-CALDENTEY ,  Joosu KUIVANEN

IPC: C12N15/82 ,  C12N15/52 ,  C12N9/10

Abstract: A recombinant host cell is disclosed for producing psilocybin and related compounds, such as metabolic intermediates of the psilocybin biosynthesis. Also provided is a method of producing psilocybin and its synthesis intermediates and related compounds, such as metabolic intermediates of the psilocybin biosynthesis, in the host cell, as well as a production system for producing them.