公开(公告)号：US20180371468A1

公开(公告)日：2018-12-27

申请号：US15737696

申请日：2017-02-21

Applicant: Teknologian tutkimuskeskus VTT Oy

Inventor： Dominik MOJZITA ,  Anssi RANTASALO ,  Jussi JÄNTTI ,  Christopher LANDOWSKI ,  Joosu KUIVANEN

IPC: C12N15/79 ,  C12N15/62

Abstract: The present invention provides an expression system for a eukaryotic host, which comprises 1) an expression cassette comprising a core promoter, said core promoter controlling the expression of a DNA sequence encoding a synthetic transcription factor (sTF), and 2) one or more expression cassettes each comprising a DNA sequence encoding a desired product operably linked to a synthetic promoter, said synthetic promoter comprising a core promoter, and sTF-specific binding sites upstream of the core promoter. The present invention also provides a method for identifying universal core promoters for eukaryotic hosts, expression systems using universal core promoters, hosts comprising said systems, and methods for producing protein products in eukaryotic hosts.

公开(公告)号：US20210010015A1

公开(公告)日：2021-01-14

申请号：US16980710

申请日：2019-03-11

Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTT OY

Inventor： Dominik MOJZITA ,  Heiko RISCHER ,  Anssi RANTASALO ,  Kirsi-Marja OKSMAN-CALDENTEY ,  Joosu KUIVANEN

IPC: C12N15/82 ,  C12N15/52 ,  C12N9/10

Abstract: A recombinant host cell is disclosed for producing psilocybin and related compounds, such as metabolic intermediates of the psilocybin biosynthesis. Also provided is a method of producing psilocybin and its synthesis intermediates and related compounds, such as metabolic intermediates of the psilocybin biosynthesis, in the host cell, as well as a production system for producing them.

公开(公告)号：US20190256875A1

公开(公告)日：2019-08-22

申请号：US16302836

申请日：2017-05-19

Applicant: TEKNOLOGIAN TUTKIMUSKESKUS VTT OY

Inventor： Joosu KUIVANEN ,  Peter RICHARD ,  Ying-Mong Jasmin WANG

IPC: C12P7/58 ,  C12R1/69 ,  C12R1/885 ,  C12N9/04 ,  C12R1/685 ,  C12P7/44 ,  C12P7/42 ,  C12N9/02

Abstract: The present invention relates to a field of genetically modified fungal cells and converting galacturonic acid to meso-galactaric acid, more precisely to a method of producing meso-galactaric acid. The invention further relates to recombinant fungal cells having a specific combination of modifications including but not limited to expression of uronate dehydrogenase enzyme, reduced D-galacturonic acid reductase activity, and furthermore reduced meso-galactaric acid catabolism, as well as uses and methods related thereto.