公开(公告)号：US11331085B2

公开(公告)日：2022-05-17

申请号：US14885598

申请日：2015-10-16

申请人： THE JOHNS HOPKINS UNIVERSITY

发明人： David Gracias ,  Kate Malachowski

IPC分类号： A61B10/02 ,  A61B10/04 ,  A61B10/06

摘要： Microgrippers adapted to capture, manipulate, and contain single cells in both in vitro and in vivo cell applications are disclosed. The energy required to actuate these microgrippers is derived from the release of residual stress and does not require any wires, tethers, or batteries. Because the microgrippers are made from biocompatible and biosorbable materials, they do not accumulate in tissue. Accordingly, they can be used for in vivo applications, such as for gripping single cells in tissue biopsies.

公开(公告)号：US20210372995A1

公开(公告)日：2021-12-02

申请号：US16758030

申请日：2018-10-22

申请人： The Johns Hopkins University

发明人： Lewis Romer ,  David Gracias ,  Jayson Pagaduan ,  Anil Bhatta ,  Xing Chen ,  Qianru Jin

IPC分类号： G01N33/50 ,  C12N5/077 ,  C12N5/071 ,  B01L3/00

摘要： In one aspect, provided is a composition (biomimetic composition) that includes a biomimetic in vitro model of an arteriolar vessel comprising: at least one of 1) human smooth muscle cells and 2) human pulmonary endothelial cells; wherein the vessel recapitulates one or more of the overall tubular geometry, morphometrics, extracellular matrix constituents, cellular morphology, cellular alignment, and functional heterotypic connections between the human smooth muscle cells and/or the human endothelial cells as compared to an in vivo arteriolar vessel. A microfluidics-based model platform of the pulmonary circulation is provided. Methods of use include measuring flow in biomimetic vessels, and to determine the resistance of these biomimetic vessels in the setting of a variety of experimental conditions that recapitulate the pathobiology of pulmonary hypertension.

公开(公告)号：US10192471B2

公开(公告)日：2019-01-29

申请号：US15093856

申请日：2016-04-08

申请人： THE JOHNS HOPKINS UNIVERSITY

发明人： David Gracias ,  Yevgeniy Kalinin ,  Shivendra Pandey ,  Jinpyo Hong

IPC分类号： G02B26/00 ,  G09G3/00

摘要： A device, system, and method for utilizing precisely patterned and chemically loaded three-dimensional porous containers akin to “chemical voxels” is disclosed to enable display of dynamic visual patterns via spatial and temporal control of both local and global chemical release. Variations in porosity, volume, shape and relative positioning of the chemical voxels can be used to control the types of images that are formed with control in both space and time. Static or moving images can be displayed using the device, system, and method of the present invention.

公开(公告)号：US20160300382A1

公开(公告)日：2016-10-13

申请号：US15093856

申请日：2016-04-08

申请人： THE JOHNS HOPKINS UNIVERSITY

发明人： David Gracias ,  Yevgeniy Kalinin ,  Shivendra Pandey ,  Jinpyo Hong

IPC分类号： G06T15/08

CPC分类号： G09G3/003

摘要： An embodiment in accordance with the present invention is directed to a device, system, and method for utilizing precisely patterned and chemically loaded three-dimensional porous containers akin to “chemical voxels” to enable dynamic visual patterns via spatial and temporal control of both local and global chemical release. Variations in porosity, volume, shape and relative positioning of the chemical voxels can be used to control the types of images that are formed with control in both space and time. Static or moving images can be displayed using the device, system, and method of the present invention.

摘要翻译： 根据本发明的实施例涉及一种用于利用类似于“化学体素”的精确图案化和化学载荷的三维多孔容器的装置，系统和方法，以通过空间和时间控制本地和 全球化学品发行。 可以使用化学体素的孔隙率，体积，形状和相对定位的变化来控制在空间和时间上由控制形成的图像的类型。 可以使用本发明的装置，系统和方法来显示静态或运动图像。