专利检索 ap:("THE JOHNS HOPKINS UNIVERSITY") AND inv:"Gabriel Roman-Melendez" 第 1 页

1.

发明授权
Protease activity profiling via programmable phage display of comprehensive proteome-scale peptide libraries 有权

公开(公告)号：US12091776B2

公开(公告)日：2024-09-17

申请号：US16913572

申请日：2020-06-26

申请人： THE JOHNS HOPKINS UNIVERSITY

发明人： Harry B. Larman , Gabriel Roman-Melendez , Thiagarajan Venkataraman

IPC分类号： C40B40/08 , C07K17/06 , C12N7/00 , C12N15/63 , C12Q1/6869

CPC分类号： C40B40/08 , C07K17/06 , C12N7/00 , C12N15/63 , C12Q1/6869 , C07K2317/34 , C07K2319/43

摘要： The present invention relates to the field of proteases. More specifically, the present invention provides compositions and methods useful for profiling protease activity using phage display. In one embodiment, a display vector useful for profiling protease activity comprises a nucleic acid sequence encoding (a) a peptide to be displayed on the surface of the vector; (b) a first affinity tag C-terminal to the peptide; and (c) a second affinity tag N-terminal to the peptide. The display vector can comprise a virus, bacteriophage, yeast, bacteria, retrovirus, ribosome or mRNA. In particular embodiments, the peptide comprises a human peptidome library peptide.