公开(公告)号：US20230159957A1

公开(公告)日：2023-05-25

申请号：US17911391

申请日：2021-03-12

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Alexander MARSON ,  Brian SHY ,  Jonathan ESENSTEN

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22

CPC classification number: C12N15/907 ,  C12N15/11 ,  C12N9/22 ,  C12N2310/20 ,  C12N2800/80

Abstract: The disclosure provides compositions and methods for modifying a target nucleic acid that use a donor construct including at least one donor template comprising a single-stranded homology directed repair template (ssHDRT) and one or more DNA-binding protein target sequences, in which at least one DNA-binding protein target sequence forms a double-stranded duplex with a complementary polynucleotide sequence.