公开(公告)号：US20210317406A1

公开(公告)日：2021-10-14

申请号：US17259085

申请日：2019-07-09

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Alexander MARSON ,  Eric SHIFRUT ,  Julia CARNEVALE ,  Alan ASHWORTH

IPC: C12N5/0783 ,  A61K35/17 ,  C12N15/11 ,  C12N15/113 ,  C12N9/22

Abstract: Provided herein are genetically modified T cells that exhibit increased proliferation compared to wild-type T cells when stimulated, methods of generating such T cells, and methods of using the T cells for the treatment of a disease such as cancer.

公开(公告)号：US20230212656A1

公开(公告)日：2023-07-06

申请号：US17801517

申请日：2021-02-22

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Eric CHOW ,  Alexander MARSON ,  Youjin LEE ,  Derek BOGDANOFF ,  Jonathan WOO ,  Chun Jimmie YE

IPC: C12Q1/6841 ,  C12Q1/6874 ,  C12N15/10

CPC classification number: C12Q1/6841 ,  C12N15/1065 ,  C12Q1/6874

Abstract: The present disclosure generally relates to spatial detection of a nucleic acid, such as a genomic DNA or a RNA transcript, in a cell comprised in a tissue sample. The present disclosure provides methods for detecting and/or analyzing nucleic acids, such as chromatin or RNA transcripts, so as to obtain spatial information about the localization, distribution or expression of genes in a tissue sample. The present disclosure thus provides a process for performing “spatial transcriptomics” or “spatial genomics,” which enables the user to determine simultaneously the expression pattern, or the location/distribution pattern of the genes expressed or genes or genomic loci present in a single cell while retaining information related to the spatial location of the cell within the tissue architecture.

公开(公告)号：US20240301403A1

公开(公告)日：2024-09-12

申请号：US18263366

申请日：2022-01-28

Applicant: The Regents of the University of California

Inventor： Daniel B. GOODMAN ,  Camillia S. AZIMI ,  Kole T. ROYBAL ,  Jeffrey A. BLUESTONE ,  Alexander MARSON

IPC: C12N15/10 ,  C12N15/86

CPC classification number: C12N15/1093 ,  C12N15/86 ,  C12N2750/14143

Abstract: This disclosure generally relates to high-throughput and modular methods and compositions for barcode-trackable cellular receptor design, manufacture, and measurement.

公开(公告)号：US20230312673A1

公开(公告)日：2023-10-05

申请号：US18043716

申请日：2021-09-01

Applicant: The Regents of the University of California

Inventor： Kole T. ROYBAL ,  Daniel B. GOODMAN ,  Camillia AZIMI ,  Alexander MARSON ,  Jeffrey A. BLUESTONE

IPC: C07K14/725 ,  C07K14/705

CPC classification number: C07K14/7051 ,  C07K14/70521 ,  C07K14/70575 ,  C07K2317/622 ,  C07K2319/03

Abstract: This disclosure relates to chimeric receptors (CRs) and their signaling components for the regulation of an immune response. Also provided are nucleic acids encoding the disclosed CRs, recombinant immune cells expressing the same, and pharmaceutical compositions containing the disclosed nucleic acids and/or recombinant cells. Further provided are methods useful for modulating an activity of an immune cell, methods for modulating an immune response in an individual, as well as methods for treating a health condition in an individual in needed thereof

公开(公告)号：US20240392243A1

公开(公告)日：2024-11-28

申请号：US18246155

申请日：2021-10-04

Applicant: The Regents of the University of California

Inventor： Theodore Lee ROTH ,  Franziska BLAESCHKE ,  Ryan APATHY ,  Alexander MARSON ,  Yan Yi Chen

IPC: C12N5/0783 ,  A61P37/04 ,  C12N15/85 ,  C12N15/86 ,  C12N15/90

Abstract: Provided herein are methods and compositions for modifying the genome of human T cells.

公开(公告)号：US20200000851A1

公开(公告)日：2020-01-02

申请号：US16568116

申请日：2019-09-11

Applicant: The Regents of the University of California

Inventor： Theodore Lee ROTH ,  Eric SHIFRUT ,  Alexander MARSON ,  Cristina Puig SAUS ,  Antoni RIBAS

IPC: A61K35/17 ,  C12N15/85 ,  C12N15/90 ,  C12N9/22 ,  C12N15/113 ,  C07K14/725

Abstract: Provided herein are methods and compositions for editing the genome of a human T cell. In some embodiments, a heterologous T cell receptor (TCR)-β chain and a heterologous TCR-α chain are inserted into exon 1 of a TCR subunit constant gene in the genome of the T cell.

公开(公告)号：US20240398862A1

公开(公告)日：2024-12-05

申请号：US17754848

申请日：2020-10-15

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY

Inventor： Jacob FREIMER ,  Alexander MARSON ,  Jonathan PRITCHARD ,  Oren SHAKED

IPC: A61K35/17 ,  A61K39/00 ,  C12N5/0783

Abstract: Provided herein are compositions and methods for modifying T cells. The disclosure is based, in part, on the use of sgRNA lentiviral infection with Cas9 protein electroporation (SLICE), to identify regulators of IL2RA, IL-2, CTLA4, arnd FOXP3 in effector T cells. IL2RA, IL-2, CTLA4, and FOXP3 are key genes in immune regulation that have been implicated in autoimmune disease and cancer. Therefore, modulating expression of these genes in T cells, for example, effector T cells or regulatory T cells, could have therapeutic applications.

公开(公告)号：US20230226183A1

公开(公告)日：2023-07-20

申请号：US17758866

申请日：2021-01-15

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Murad R. MAMEDOV ,  Alexander MARSON

IPC: A61K39/00 ,  C12N15/90

CPC classification number: A61K39/4633 ,  A61K39/4611 ,  C12N15/907 ,  C12N2310/20

Abstract: Provided herein are compositions and methods for altering sensitivity of target cells to killing by γδ T cells.

公开(公告)号：US20230159957A1

公开(公告)日：2023-05-25

申请号：US17911391

申请日：2021-03-12

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Alexander MARSON ,  Brian SHY ,  Jonathan ESENSTEN

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22

CPC classification number: C12N15/907 ,  C12N15/11 ,  C12N9/22 ,  C12N2310/20 ,  C12N2800/80

Abstract: The disclosure provides compositions and methods for modifying a target nucleic acid that use a donor construct including at least one donor template comprising a single-stranded homology directed repair template (ssHDRT) and one or more DNA-binding protein target sequences, in which at least one DNA-binding protein target sequence forms a double-stranded duplex with a complementary polynucleotide sequence.

公开(公告)号：US20220241336A1

公开(公告)日：2022-08-04

申请号：US17725478

申请日：2022-04-20

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Theodore Lee ROTH ,  Eric SHIFRUT ,  Alexander MARSON ,  Cristina PUIG SAUS ,  Antoni RIBAS

IPC: A61K35/17 ,  C07K14/725 ,  C12N9/22 ,  C12N15/113 ,  C12N15/85 ,  C12N15/90

Abstract: Provided herein are methods and compositions for editing the genome of a human T cell. In some embodiments, a heterologous T cell receptor (TCR)-β chain and a heterologous TCR-α chain are inserted into exon 1 of a TCR subunit constant gene in the genome of the T cell.