公开(公告)号：US20230415157A1

公开(公告)日：2023-12-28

申请号：US18030959

申请日：2021-10-11

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Jered Haun ,  Jeremy A. Lombardo

IPC: B01L3/00 ,  C12M3/06 ,  C12M1/00

CPC classification number: B01L3/502753 ,  C12M23/16 ,  C12M45/09 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0622 ,  C12Y304/24007 ,  B01L2300/0681

Abstract: A microfluidic system for processing a tissue sample includes a microfluidic digestion device having an outlet fluidically connected to an inlet of a dissociation/filter device. The microfluidic digestion device includes an inlet and an outlet and a tissue chamber that connects to plurality of upstream fluidic channels and a plurality of downstream fluidic channels. The microfluidic dissociation/filter device includes an inlet, a first outlet, a second outlet, and a plurality of furcating dissociation channels having a plurality of expansion and constriction regions disposed along a length thereof, wherein one or more filters are disposed in a flow path downstream of the plurality of furcating dissociation channels. Pumps are provided to pump buffer and/or enzyme-containing fluid through the digestion device and dissociation/filter device. Tissue is initially processed in the digestion device and then passes into the dissociation/filter device.

公开(公告)号：US20220249761A1

公开(公告)日：2022-08-11

申请号：US17617301

申请日：2020-06-23

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Jered Haun ,  Jeremy A. Lombardo

IPC: A61M1/00 ,  B01L3/00 ,  A61K35/35

Abstract: An integrated lipoaspirate processing system is disclosed for mechanically processing adipose tissue into a therapeutic material that, in some embodiments, may be directly injected into a subject. The system includes an emulsification device that is used to emulsify the lipoaspirate sample. The emulsified lipoaspirate is then filtered by a microfluidic filtration device. The filtrate of the microfluidic filtration device is then processed in a microfluidic dissociation device. The integrated platform or system helps standardize hydrodynamic processing of lipoaspirate by producing predictable and consistent shear forces and enabling automation in clinical settings. Progressive processing through multiple devices in series enables optimal recovery of regenerative cells while preventing clogging.