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公开(公告)号：US11427798B2
公开(公告)日：2022-08-30
申请号：US16868474
申请日：2020-05-06
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Janice De Jesus , Elliot En-Yu Hui , Jiang Li
Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.
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公开(公告)号：US10926257B2
公开(公告)日：2021-02-23
申请号：US16115434
申请日：2018-08-28
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Xiaolong Qiu , Elliot Hui , Amrith Karunaratne , Erik Werner
Abstract: A microfluidic device uses hydrodynamic shear forces on a sample to improve the speed and efficiency of tissue digestion is disclosed. The microfluidic channels are designed to apply hydrodynamic shear forces at discrete locations on tissue specimens up to 1 cm in length and 1 mm in diameter, thereby accelerating digestion through hydrodynamic shear forces and improved enzyme-tissue contact. Experiments using animal organs show that the digestion device with hydro-mincing capabilities is superior to conventional scalpel mincing and digestion based on recovery of DNA and viable single cells. The microfluidic digestion device can eliminate or reduce the need to mince tissue samples with a scalpel, while reducing sample processing time and preserving cell viability. Another advantage is that downstream microfluidic operations could be integrated to enable advanced cell processing and analysis capabilities. The device may be used in research and clinical settings to promote single cell-based analysis technologies, as well as to isolate primary, progenitor, and stem cells for use in the fields of tissue engineering and regenerative medicine.
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公开(公告)号：US12292046B2
公开(公告)日：2025-05-06
申请号：US17058634
申请日：2019-05-29
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Xiaolong Qiu , Marissa Noelani Pennell , Elliott E. Hui
Abstract: A microfluidic tissue dissociation and filtration device simultaneously filters large tissue fragments and dissociates smaller aggregates into single cells, thereby improving single cell yield and purity. The device includes an inlet coupled to a first microfluidic channel at an upstream location and a first outlet at a downstream location. A first filter membrane is interposed between the first microfluidic channel and a second microfluidic channel, wherein the second microfluidic channel is in fluidic communication with the first microfluidic channel via the first filter membrane. The first filter membrane operates under a tangential flow format. A second outlet is coupled to a downstream location of the second microfluidic channel and includes a second filter membrane interposed between the second outlet and the second microfluidic channel. The dual membrane device increased single cell numbers by at least 3-fold for different tissue types.
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公开(公告)号：US20200283723A1
公开(公告)日：2020-09-10
申请号：US16868474
申请日：2020-05-06
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Janice De Jesus , Elliot En-Yu Hui , Jiang Li
Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.
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公开(公告)号：US09580678B2
公开(公告)日：2017-02-28
申请号：US14309720
申请日：2014-06-19
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Janice De Jesus , Elliot En-Yu Hui , Jiang Li
CPC classification number: G01N1/286 , B01L3/502753 , B01L2300/0816 , B01L2300/0864 , B01L2300/0887 , B01L2400/0487 , B01L2400/086 , C12M21/08 , C12M21/18 , C12M45/02 , C12M47/04
Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.
Abstract translation: 组织解离装置包括连接到具有具有上游端和下游端的单个通道的第一级的入口; 多个串联布置的中间级，其中多个第一中间级流体耦合到第一级的下游端，并且其中多个的每个后续中间级具有越来越多的通道（具有较小尺寸的通道） ; 以及连接到中间级的最后阶段的出口。
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Patent Agency Ranking
公开(公告)号：US20140377866A1
公开(公告)日：2014-12-25
申请号：US14309720
申请日：2014-06-19
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Janice De Jesus , Elliot En-Yu Hui , Jiang Li
CPC classification number: G01N1/286 , B01L3/502753 , B01L2300/0816 , B01L2300/0864 , B01L2300/0887 , B01L2400/0487 , B01L2400/086 , C12M21/08 , C12M21/18 , C12M45/02 , C12M47/04
Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.
Abstract translation: 组织解离装置包括连接到具有具有上游端和下游端的单个通道的第一级的入口; 多个串联布置的中间级，其中多个第一中间级流体耦合到第一级的下游端，并且其中多个的每个后续中间级具有越来越多的通道（具有较小尺寸的通道） ; 以及连接到中间级的最后阶段的出口。
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公开(公告)号：US12036550B2
公开(公告)日：2024-07-16
申请号：US17180711
申请日：2021-02-19
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Xiaolong Qiu , Elliot Hui , Amrith Karunaratne , Erik Werner
IPC: B01L3/00
CPC classification number: B01L3/50273 , B01L2200/027 , B01L2200/0647 , B01L2300/042 , B01L2300/0816
Abstract: A microfluidic device uses hydrodynamic shear forces on a sample to improve the speed and efficiency of tissue digestion is disclosed. The microfluidic channels are designed to apply hydrodynamic shear forces at discrete locations on tissue specimens up to 1 cm in length and 1 mm in diameter, thereby accelerating digestion through hydrodynamic shear forces and improved enzyme-tissue contact. The microfluidic digestion device can eliminate or reduce the need to mince tissue samples with a scalpel, while reducing sample processing time and preserving cell viability. Another advantage is that downstream microfluidic operations could be integrated to enable advanced cell processing and analysis capabilities. The device may be used in research and clinical settings to promote single cell-based analysis technologies, as well as to isolate primary, progenitor, and stem cells for use in the fields of tissue engineering and regenerative medicine.
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公开(公告)号：US10683480B2
公开(公告)日：2020-06-16
申请号：US15413201
申请日：2017-01-23
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Janice De Jesus , Elliot En-Yu Hui , Jiang Li
Abstract: A tissue dissociation device includes an inlet coupled to a first stage having a single channel having an upstream end and a downstream end; a plurality of serially arranged intermediate stages, wherein a first intermediate stage of the plurality is fluidically coupled to the downstream end of the first stage, and wherein each subsequent intermediate stage of the plurality has an increasing number of channels (with channels of smaller dimensions); and an outlet coupled to a last stage of the intermediate stages.
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公开(公告)号：US20200164374A1
公开(公告)日：2020-05-28
申请号：US16777795
申请日：2020-01-30
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Ahmed Zobi , Justin Stovner , Hugo Salas , David Duarte , Jered Haun , Alan Widgerow , Derek Banyard
Abstract: Provided herein are devices and methods of processing a sample that include, in several embodiments, rotating one or more microfluidic chips that are mounted on a support plate using a motor driven rotational chuck. By spinning one or more of the microfluidic chips about a common center of rotation in a controlled manner, high flow rates (and high shear forces) are imparted to the sample in a controlled manner. Each microfluidic chip can be rotated 180° on the support plate so that the sample can be run back-and-forth through the microfluidic devices. Because the support plate can be driven at relatively high RPMs, high flow rates are generated within the microfluidic chips. This increases the shear forces on the sample and also decreases the processing time involved as the sample can quickly pass through the shear-inducing features of the microfluidic chip(s).
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公开(公告)号：US20180361382A1
公开(公告)日：2018-12-20
申请号：US16101254
申请日：2018-08-10
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Ahmed Zobi , Justin Stovner , Hugo Salas , David Duarte , Jered Haun , Alan Widgerow , Derek Banyard
Abstract: Provided herein are devices and methods of processing a sample that include, in several embodiments, rotating one or more microfluidic chips that are mounted on a support plate using a motor driven rotational chuck. By spinning one or more of the microfluidic chips about a common center of rotation in a controlled manner, high flow rates (and high shear forces) are imparted to the sample in a controlled manner. Each microfluidic chip can be rotated 180° on the support plate so that the sample can be run back-and-forth through the microfluidic devices. Because the support plate can be driven at relatively high RPMs, high flow rates are generated within the microfluidic chips. This increases the shear forces on the sample and also decreases the processing time involved as the sample can quickly pass through the shear-inducing features of the microfluidic chip(s).
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