公开(公告)号：US11385232B2

公开(公告)日：2022-07-12

申请号：US16641955

申请日：2018-08-31

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Vatche Agopian ,  Hsian-Rong Tseng ,  Shuang Hou

IPC: G01N33/574 ,  C07K16/28 ,  C07K16/30 ,  G01N33/543

Abstract: Methods and kits for detecting hepatocellular carcinoma recurrence or metastasis, and of measuring markers of hepatocellular carcinoma, including markers of hepatocellular carcinoma recurrence or metastasis, in a blood sample obtained from a subject by (a) isolating circulating tumor cells (CTCs) by contacting a blood sample obtained from the subject with a set of capture antibodies, wherein the capture antibodies specifically bind asialoglycoprotein receptor (ASGPR), Glypican-3, and epithelial cell adhesion molecule (EpCAM); (b) contacting the isolated CTCs with an antibody that specifically binds vimentin; and (c) measuring the number of vimentin-positive CTC.