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    SYSTEMS, METHODS AND COMPONENTS FOR ISOLATING CELLS FROM A FLUID SAMPLE
    3.
    发明申请
    SYSTEMS, METHODS AND COMPONENTS FOR ISOLATING CELLS FROM A FLUID SAMPLE 审中-公开
    用于从流体样品中分离细胞的系统，方法和组分

    公开(公告)号：US20150285717A1

    公开(公告)日：2015-10-08

    申请号：US14403542

    申请日：2013-05-29

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  CYTOLUMINA TECHNOLOGIES CORP.

    Inventor： Hsian-Rong Tseng ,  Mitch A. Garcia ,  Min Song ,  Libo Zhao ,  Shuang Hou ,  Tom Lee ,  Daniel Wang ,  Bo Tao

    IPC: G01N1/28 ,  B01L3/00

    CPC classification number: G01N1/28 ,  B01L3/502715 ,  B01L3/502761 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2300/0609 ,  B01L2300/0816 ,  B01L2300/0883 ,  G01N1/286 ,  G01N1/4077 ,  G01N33/56966 ,  G01N33/574 ,  G01N2001/284 ,  G01N2001/2886

    Abstract: A system for isolating preselected cell types from a fluid sample that includes a plurality of cell types includes a cell-capture fluidic chip, and a chip holder configured to receive the cell-capture fluidic chip and to maintain the cell-capture fluidic chip with a substantially fluid-tight seal while in operation. The chip holder is further configured to release the cell-capture fluidic chip to be removed from the chip holder for further processing. The cell-capture fluidic chip includes a substrate, a laser micro-dissection membrane disposed on the substrate, and a channel-defining layer disposed on the laser micro-dissection membrane. The laser micro-dissection membrane has a surface adapted to capture preselected cell types preferentially over other cell types of the plurality of cell types. The channel-defining layer is removable from the laser micro-dissection membrane for further processing of the cell-capture fluidic chip.124

    Abstract translation: 用于从包括多个细胞类型的流体样品中分离预选细胞类型的系统包括细胞捕获流体芯片和被配置为接收细胞捕获流体芯片并且将细胞捕获流体芯片维持在 在运行时基本上是流体密封的。 芯片保持器进一步构造成释放要从芯片保持器移除的细胞捕获流体芯片用于进一步处理。 细胞捕获流体芯片包括基板，设置在基板上的激光微解剖膜和设置在激光微分离膜上的通道限定层。 激光微解剖膜具有适于比多种细胞类型的其它细胞类型优先捕获预选细胞类型的表面。 通道限定层可从激光微解剖膜移除，用于细胞捕获流体芯片的进一步处理。 124

    GUIDED MAGNETIC NANOSTRUCTURES FOR TARGETED AND HIGH-THROUGHPUT INTRACELLULAR DELIVERY
    10.
    发明申请
    GUIDED MAGNETIC NANOSTRUCTURES FOR TARGETED AND HIGH-THROUGHPUT INTRACELLULAR DELIVERY 审中-公开

    公开(公告)号：US20200347409A1

    公开(公告)日：2020-11-05

    申请号：US16962509

    申请日：2019-01-29

    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

    Inventor： Paul S. Weiss ,  Hsian-Rong Tseng ,  Xiaobin Xu ,  Natcha Wattanatorn ,  Qing Yang ,  Steven J. Jonas

    IPC: C12N15/87 ,  G01N33/543

    Abstract: A method of transporting biomolecular cargo intracellularly into cells includes the operations of providing magnetic nanostructures (e.g., nanospears, nanostars, nanorods, and other nanometer-sized structures) carrying the biomolecular cargo thereon and applying an external magnetic field to move the magnetic nanostructures into physical contact with at least some of the cells (or the cells into the magnetic nanostructures). The magnetic nanostructures move into physical contact with a single cell, a subset of cells, or all cells. The external magnetic field may be applied by a moving permanent magnet although an electromagnetic may also be used. The biomolecular cargo may include a molecule, a plurality of molecules, or higher order biological constructs. For example, the biological construct may include DNA plasmids, small interfering RNA, proteins, or targeted nuclease gene-editing cargo such as zinc-finger nucleases, transcription activator-like effector nucleases, Cas9 protein, Cas9 mRNA, and associated guide RNA sequences.

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