公开(公告)号：US20230212656A1

公开(公告)日：2023-07-06

申请号：US17801517

申请日：2021-02-22

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Eric CHOW ,  Alexander MARSON ,  Youjin LEE ,  Derek BOGDANOFF ,  Jonathan WOO ,  Chun Jimmie YE

IPC: C12Q1/6841 ,  C12Q1/6874 ,  C12N15/10

CPC classification number: C12Q1/6841 ,  C12N15/1065 ,  C12Q1/6874

Abstract: The present disclosure generally relates to spatial detection of a nucleic acid, such as a genomic DNA or a RNA transcript, in a cell comprised in a tissue sample. The present disclosure provides methods for detecting and/or analyzing nucleic acids, such as chromatin or RNA transcripts, so as to obtain spatial information about the localization, distribution or expression of genes in a tissue sample. The present disclosure thus provides a process for performing “spatial transcriptomics” or “spatial genomics,” which enables the user to determine simultaneously the expression pattern, or the location/distribution pattern of the genes expressed or genes or genomic loci present in a single cell while retaining information related to the spatial location of the cell within the tissue architecture.