公开(公告)号：US20200025783A1

公开(公告)日：2020-01-23

申请号：US16518495

申请日：2019-07-22

Applicant: Scienion AG ,  The Regents of the University of California

Inventor： Derek BOGDANOFF ,  Eric CHOW ,  Michael WILSON ,  Ariele Lauren GREENFIELD ,  Joshua CANTLON-BRUCE

IPC: G01N35/10 ,  B01L3/02 ,  C12M1/00

Abstract: A particle isolation apparatus 100 for isolating particles from a suspension sample, includes a droplet dispenser device 10 for collecting the suspension sample from a carrier substrate 20 and for dispensing droplets onto a target substrate 30, a mechanical pump device 40 being coupled with the droplet dispenser device 10 for loading a dilution liquid into the droplet dispenser device 10 and for aspirating a first portion of the suspension sample into the droplet dispenser device 10, and a syphon pump device 50 being coupled with the droplet dispenser device 10 and being arranged for aspirating a second portion of the suspension sample into the droplet dispenser device 10. Preferably, the droplet dispenser device 10 is configured for dispensing single particle droplets on the target substrate 30. Furthermore, a method of isolating particles from a suspension sample is described.

公开(公告)号：US20230212656A1

公开(公告)日：2023-07-06

申请号：US17801517

申请日：2021-02-22

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Eric CHOW ,  Alexander MARSON ,  Youjin LEE ,  Derek BOGDANOFF ,  Jonathan WOO ,  Chun Jimmie YE

IPC: C12Q1/6841 ,  C12Q1/6874 ,  C12N15/10

CPC classification number: C12Q1/6841 ,  C12N15/1065 ,  C12Q1/6874

Abstract: The present disclosure generally relates to spatial detection of a nucleic acid, such as a genomic DNA or a RNA transcript, in a cell comprised in a tissue sample. The present disclosure provides methods for detecting and/or analyzing nucleic acids, such as chromatin or RNA transcripts, so as to obtain spatial information about the localization, distribution or expression of genes in a tissue sample. The present disclosure thus provides a process for performing “spatial transcriptomics” or “spatial genomics,” which enables the user to determine simultaneously the expression pattern, or the location/distribution pattern of the genes expressed or genes or genomic loci present in a single cell while retaining information related to the spatial location of the cell within the tissue architecture.

公开(公告)号：US20220275438A1

公开(公告)日：2022-09-01

申请号：US17625687

申请日：2020-07-08

Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Zev GARTNER ,  David PATTERSON ,  Eric CHOW ,  Robert WEBER ,  Christopher MCGINNIS

IPC: C12Q1/6869 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q1/6806

Abstract: The disclosure generally relates to methods and applications of single-cell barcoding and methods of nucleotide sequencing using composition comprising lipid-modified oligonucleotides.