公开(公告)号：US11536659B2

公开(公告)日：2022-12-27

申请号：US16473584

申请日：2017-12-27

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Xudong Fan ,  Yu-Cheng Chen ,  Qiushu Chen

IPC: G01N21/64 ,  G02B21/00 ,  G02B21/34

Abstract: Laser emission based microscope devices and methods of using such devices for detecting laser emissions from a tissue sample are provided. The scanning microscope has first and second reflection surfaces and a scanning cavity holding a stationary tissue sample with at least one fluorophore/lasing energy responsive species. At least a portion of the scanning cavity corresponds to a high quality factor (Q) Fabry-Pérot resonator cavity. A lasing pump source directs energy at the scanning cavity while a detector receives and detects emissions generated by the fluorophore(s) or lasing energy responsive species. The second reflection surface and/or the lasing pump source are translatable with respect to the stationary tissue sample for generating a two-dimensional scan of the tissue sample. Methods for detecting multiplexed emissions or quantifying one or more biomarkers in a histological tissue sample, for example for detection and diagnosis of cancer, or other disorders/diseases are provided.

公开(公告)号：US12000782B2

公开(公告)日：2024-06-04

申请号：US17421809

申请日：2020-01-09

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN

Inventor： Xudong Fan ,  Xueding Wang ,  Xuzhou Li ,  Yu Qin ,  Xiaotian Tan ,  Yu-Cheng Chen ,  Qiushu Chen

IPC: G01N21/63 ,  A61B5/00 ,  H01S3/08 ,  H01S3/083 ,  H01S3/094

CPC classification number: G01N21/63 ,  A61B5/6876 ,  H01S3/08059 ,  H01S3/083 ,  H01S3/094076 ,  G01N2201/06113

Abstract: A laser whose emission is modulated by ultrasound is presented. The laser is usually micron-sized. In response to ultrasound modulation, the laser emission increases and decreases. Such a change in emission can be detected by external optical detectors. This type of laser can be used as a new type of imaging modality, in which laser emission in combination with sound waves or ultrasound waves, is used for imaging Laser emission has a much narrower spectral linewidth and stronger intensity than fluorescence and therefore is able to achieve higher sensitivity, whereas sound waves are used to provide a better spatial resolution of the laser emission from the laser. In ultrasound modulated laser based imaging, multiple lasers can be placed inside cells or tissues.