公开(公告)号：US11262355B2

公开(公告)日：2022-03-01

申请号：US16708302

申请日：2019-12-09

申请人： THE TRUSTEES OF DARTMOUTH COLLEGE

发明人： John B. Weaver ,  Barjor Gimi ,  Karl Griswold

IPC分类号： G01N33/543 ,  G01N27/74 ,  G01R33/12 ,  A61B5/055

摘要： A system for measuring analyte concentrations has porous-walled nanocontainers containing multiple magnetic nanoparticles, the magnetic nanoparticles coated with a selective binder that is analyte-responsive and binds a the analyte, an indicator substance releasable from the selective binder by the analyte, or an indicator substance cleavable by the analyte, apparatus for exposing the nanocontainers to a fluid potentially containing the analyte, and magnetic spectroscopy of Brownian motion sensing apparatus for detecting agglutination of the nanoparticles or binding of analyte to the nanoparticles. The system is used in a method comprising coating magnetic nanoparticles with a selective binder, encapsulating the magnetic nanoparticles in porous nanocontainers, exposing the nanocontainers to a fluid potentially containing analyte, using magnetic spectroscopy of Brownian motion sensing apparatus to detect agglutination or binding of the nanoparticles, and translating Brownian motion spectra to analyte concentrations.

公开(公告)号：US20150148661A1

公开(公告)日：2015-05-28

申请号：US14408041

申请日：2013-06-14

申请人： The Trustees of Dartmouth College

发明人： John B. Weaver ,  Barjor Gimi ,  Karl Griswold

IPC分类号： G01N33/543 ,  G01N27/74 ,  A61B5/055

CPC分类号： G01N33/54366 ,  A61B5/055 ,  G01N27/745 ,  G01N33/54326 ,  G01R33/1269

摘要： A system for measuring analyte concentrations has porous-walled nanocontainers containing multiple magnetic nanoparticles, the magnetic nanoparticles coated with a selective binder that is analyte-responsive and binds a the analyte, an indicator substance releasable from the selective binder by the analyte, or an indicator substance cleavable by the analyte, apparatus for exposing the nanocontainers to a fluid potentially containing the analyte, and magnetic spectroscopy of Brownian motion sensing apparatus for detecting agglutination of the nanoparticles or binding of analyte to the nanoparticles. The system is used in a method comprising coating magnetic nanoparticles with a selective binder, encapsulating the magnetic nanoparticles in porous nanocontainers, exposing the nanocontainers to a fluid potentially containing analyte, using magnetic spectroscopy of Brownian motion sensing apparatus to detect agglutination or binding of the nanoparticles, and translating Brownian motion spectra to analyte concentrations.

摘要翻译： 用于测量分析物浓度的系统具有包含多个磁性纳米颗粒的多孔壁纳米容器，所述磁性纳米粒子涂覆有选择性粘合剂，所述选择性粘合剂是分析物响应性的并且结合分析物，可由分析物从选择性粘合剂释放的指示剂物质，或指示剂 被分析物可分离的物质，用于将纳米容器暴露于潜在含有分析物的流体的装置，以及用于检测纳米颗粒的凝集或者将分析物结合到纳米颗粒的布朗运动检测装置的磁光谱。 该系统用于包括用选择性粘合剂涂覆磁性纳米颗粒的方法，将磁性纳米颗粒包封在多孔纳米容器中，使用布朗运动感测装置的磁光谱法将纳米容器暴露于潜在含有分析物的流体中，以检测纳米颗粒的凝集或结合 ，并将布朗运动光谱转换为分析物浓度。