公开(公告)号：US20200283507A1

公开(公告)日：2020-09-10

申请号：US16854703

申请日：2020-04-21

申请人： THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Servic ,  INSTITUTO NACIONAL DE TECNOLOGIA AGROPECUARIA

发明人： Karin Bok ,  Lorena Laura Garaicoechea ,  Viviana Parreno ,  Andrea Pamela Aguilar ,  Marina Bok ,  Lisbeth Kim Green ,  Stanislav Vladimirovich Sosnovtsev

IPC分类号： C07K16/10 ,  G01N33/569

摘要： Isolated VHH monoclonal antibodies are disclosed that specifically bind to a Norovirus polypeptide. In some embodiments, the Norovirus is a Genogroup I Norovirus or a Genogroup II Norovirus. In other embodiments, the Norovirus is Norwalk or MD2004 virus. In some embodiments, the monoclonal antibodies specifically bind VP1. Also disclosed are compositions including the disclosed antibodies, nucleic acids encoding these antibodies, expression vectors including the nucleic acids, and isolated host cells that express the nucleic acids. The antibodies and compositions disclosed herein can be used for detecting the presence of a Norovirus in a biological sample, or detecting a Norovirus infection. Also disclosed are methods of treating and/or preventing a NoV infection.

公开(公告)号：US20180251527A1

公开(公告)日：2018-09-06

申请号：US15982998

申请日：2018-05-17

申请人： THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Serv ,  INSTITUTO NACIONAL DE TECNOLOGIA AGROPECUARIA

发明人： Karin Bok ,  Lorena Laura Garaicoechea ,  Viviana Parreno ,  Andrea Pamela Aguilar ,  Marina Bok ,  Lisbeth Kim Green ,  Stanislav Vladimirovich Sosnovtsev

IPC分类号： C07K16/10 ,  G01N33/569 ,  A61K39/00

摘要： Isolated VHH monoclonal antibodies are disclosed that specifically bind to a Norovirus polypeptide. In some embodiments, the Norovirus is a Genogroup I Norovirus or a Genogroup II Norovirus. In other embodiments, the Norovirus is Norwalk or MD2004 virus. In some embodiments, the monoclonal antibodies specifically bind VP1. Also disclosed are compositions including the disclosed antibodies, nucleic acids encoding these antibodies, expression vectors including the nucleic acids, and isolated host cells that express the nucleic acids. The antibodies and compositions disclosed herein can be used for detecting the presence of a Norovirus in a biological sample, or detecting a Norovirus infection. Also disclosed are methods of treating and/or preventing a NoV infection.

公开(公告)号：US11325965B2

公开(公告)日：2022-05-10

申请号：US16854703

申请日：2020-04-21

申请人： THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Services ,  INSTITUTO NACIONAL DE TECNOLOGIA AGROPECUARIA

发明人： Karin Bok ,  Lorena Laura Garaicoechea ,  Viviana Parreno ,  Andrea Pamela Aguilar ,  Marina Bok ,  Lisbeth Kim Green ,  Stanislav Vladimirovich Sosnovtsev

IPC分类号： G01N33/569 ,  C07K16/10 ,  A61K39/00

摘要： Isolated VHH monoclonal antibodies are disclosed that specifically bind to a Norovirus polypeptide. In some embodiments, the Norovirus is a Genogroup I Norovirus or a Genogroup II Norovirus. In other embodiments, the Norovirus is Norwalk or MD2004 virus. In some embodiments, the monoclonal antibodies specifically bind VP1. Also disclosed are compositions including the disclosed antibodies, nucleic acids encoding these antibodies, expression vectors including the nucleic acids, and isolated host cells that express the nucleic acids. The antibodies and compositions disclosed herein can be used for detecting the presence of a Norovirus in a biological sample, or detecting a Norovirus infection. Also disclosed are methods of treating and/or preventing a NoV infection.

公开(公告)号：US10662238B2

公开(公告)日：2020-05-26

申请号：US15982998

申请日：2018-05-17

申请人： THE UNITED STATES OF AMERICA, as represented by the Secretary, Department of Health and Human Services ,  INSTITUTO NACIONAL DE TECNOLOGIA AGROPECUARIA

发明人： Karin Bok ,  Lorena Laura Garaicoechea ,  Viviana Parreno ,  Andrea Pamela Aguilar ,  Marina Bok ,  Lisbeth Kim Green ,  Stanislav Vladimirovich Sosnovtsev

IPC分类号： C07K16/10 ,  G01N33/569 ,  A61K33/44 ,  A61K39/00

摘要： Isolated VHH monoclonal antibodies are disclosed that specifically bind to a Norovirus polypeptide. In some embodiments, the Norovirus is a Genogroup I Norovirus or a Genogroup II Norovirus. In other embodiments, the Norovirus is Norwalk or MD2004 virus. In some embodiments, the monoclonal antibodies specifically bind VP1. Also disclosed are compositions including the disclosed antibodies, nucleic acids encoding these antibodies, expression vectors including the nucleic acids, and isolated host cells that express the nucleic acids. The antibodies and compositions disclosed herein can be used for detecting the presence of a Norovirus in a biological sample, or detecting a Norovirus infection. Also disclosed are methods of treating and/or preventing a NoV infection.

公开(公告)号：US20160102136A1

公开(公告)日：2016-04-14

申请号：US14889774

申请日：2014-05-09

申请人： THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPT. OF HEALTH AND HUMAN SERVICES ,  INSTITUTO NACIONAL DE TECNOLOGIA AGROPECUARIA

发明人： Karin Bok ,  Lorena Laura Garaicoechea ,  Viviana Parreno ,  Andrea Pamela Aguilar ,  Marina Bok ,  Lisbeth Kim Green ,  Stanislav Vladimirovich Sosnovtsev

IPC分类号： C07K16/10 ,  G01N33/569

CPC分类号： C07K16/10 ,  A61K2039/505 ,  C07K2317/22 ,  C07K2317/24 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2317/92 ,  C12N2770/16011 ,  C12N2770/16034 ,  G01N33/56983 ,  G01N2333/08

摘要： Isolated VHH monoclonal antibodies are disclosed that specifically bind to a Norovirus polypeptide. In some embodiments, the Norovirus is a Genogroup I Norovirus or a Genogroup II Norovirus. In other embodiments, the Norovirus is Norwalk or MD2004 virus. In some embodiments, the monoclonal antibodies specifically bind VP1. Also disclosed are compositions including the disclosed antibodies, nucleic acids encoding these antibodies, expression vectors including the nucleic acids, and isolated host cells that express the nucleic acids. The antibodies and compositions disclosed herein can be used for detecting the presence of a Norovirus in a biological sample, or detecting a Norovirus infection. Also disclosed are methods of treating and/or preventing a NoV infection.

摘要翻译： 公开了特异性结合诺如病毒多肽的分离的VHH单克隆抗体。 在一些实施方案中，诺如病毒是Genogroup I诺如病毒或Genogroup II诺如病毒。 在其他实施方案中，诺如病毒是Norwalk或MD2004病毒。 在一些实施方案中，单克隆抗体特异性结合VP1。 还公开了包括所公开的抗体，编码这些抗体的核酸，包括核酸的表达载体和表达核酸的分离的宿主细胞的组合物。 本文公开的抗体和组合物可用于检测生物样品中诺如病毒的存在或检测诺如病毒感染。 还公开了治疗和/或预防NoV感染的方法。

公开(公告)号：US10000556B2

公开(公告)日：2018-06-19

申请号：US14889774

申请日：2014-05-09

申请人： THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPART OF HEALTH AND HUMAN SERVICES ,  INSTITUTO NACIONAL DE TECNOLOGIA AGROPECUARIA

发明人： Karin Bok ,  Lorena Laura Garaicoechea ,  Viviana Parreno ,  Andrea Pamela Aguilar ,  Marina Bok ,  Lisbeth Kim Green ,  Stanislav Vladimirovich Sosnovtsev

IPC分类号： C07K16/10 ,  G01N33/569 ,  A61K39/00

CPC分类号： C07K16/10 ,  A61K2039/505 ,  C07K2317/22 ,  C07K2317/24 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/569 ,  C07K2317/76 ,  C07K2317/92 ,  C12N2770/16011 ,  C12N2770/16034 ,  G01N33/56983 ,  G01N2333/08

摘要： Isolated VHH monoclonal antibodies are disclosed that specifically bind to a Norovirus polypeptide. In some embodiments, the Norovirus is a Genogroup I Norovirus or a Genogroup II Norovirus. In other embodiments, the Norovirus is Norwalk or MD2004 virus. In some embodiments, the monoclonal antibodies specifically bind VP1. Also disclosed are compositions including the disclosed antibodies, nucleic acids encoding these antibodies, expression vectors including the nucleic acids, and isolated host cells that express the nucleic acids. The antibodies and compositions disclosed herein can be used for detecting the presence of a Norovirus in a biological sample, or detecting a Norovirus infection. Also disclosed are methods of treating and/or preventing a NoV infection.