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公开(公告)号：US20210087515A1
公开(公告)日：2021-03-25
申请号：US17044083
申请日：2019-05-15
Inventor： Nancy L. Allbritton , Yuli Wang , Raehyun Kim , Peter Joseph Attayek
Abstract: Disclosed herein are devices, apparatuses and methods for generating self-sustaining gaseous and non-gaseous gradients across a cell support structure and for culturing one or more cells or tissues under hypoxic conditions. Methods of generating one or more gas gradients across a cell support structure include providing a luminal container having a bottom wall that is a gas permeable membrane, positioning a cell support structure above the bottom wall, positioning one or more cells or tissues on the cell support structure, and generating one or more gas gradients between the bottom wall, across the cell support structure and into a luminal reservoir. An apparatus to produce hypoxic conditions for cell cultures includes a luminal container having a bottom wall, a cell support structure on the bottom wall, and a cover that sealably closes the open top, such that a hypoxic condition can be generated in the luminal reservoir.
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Patent Agency Ranking
公开(公告)号：US20230098968A1
公开(公告)日：2023-03-30
申请号：US17044157
申请日：2019-05-24
Inventor： Nancy L. Allbritton , Yuli Wang , Raehyun Kim
Abstract: A method for producing tissue constructs comprising two or more distinct regions, each of which comprises a different cell population or lineage is described. The method involves providing a support substrate or substrate assembly comprising two or more physically distinct regions, wherein the two or more physically distinct regions of the support substrate or substrate assembly are different from each other; and depositing/positioning one or more cells on the support substrate or substrate assembly. The cells can form a continuous monolayer with at least two zones, e.g., a proliferative zone and a nonproliferative zone, that can act as in vitro intestinal models. The models are two-dimensional, thus facilitating rapid and facile imaging Systems comprising the tissue constructs and methods of using the constructs to study the effects of pharmaceuticals, uutraceuticais, and metabolites on intestinal cells are also described.
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公开(公告)号：US20250027027A1
公开(公告)日：2025-01-23
申请号：US18786858
申请日：2024-07-29
Inventor： Nancy L. Allbritton , Yuli Wang , Raehyun Kim , Peter Joseph Attayek
Abstract: Disclosed herein are devices, apparatuses and methods for generating self-sustaining gaseous and non-gaseous gradients across a cell support structure and for culturing one or more cells or tissues under hypoxic conditions. Methods of generating one or more gas gradients across a cell support structure include providing a luminal container having a bottom wall that is a gas permeable membrane, positioning a cell support structure above the bottom wall, positioning one or more cells or tissues on the cell support structure, and generating one or more gas gradients between the bottom wall, across the cell support structure and into a luminal reservoir. An apparatus to produce hypoxic conditions for cell cultures includes a luminal container having a bottom wall, a cell support structure on the bottom wall, and a cover that sealably closes the open top, such that a hypoxic condition can be generated in the luminal reservoir.
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公开(公告)号：US12195706B2
公开(公告)日：2025-01-14
申请号：US18371636
申请日：2023-09-22
Inventor： Nancy L. Allbritton , Yuli Wang , Raehyun Kim
Abstract: A method for producing tissue constructs comprising two or more distinct regions, each of which comprises a different cell population or lineage is described. The method involves providing a support substrate or substrate assembly comprising two or more physically distinct regions, wherein the two or more physically distinct regions of the support substrate or substrate assembly are different from each other; and depositing/positioning one or more cells on the support substrate or substrate assembly. The cells can form a continuous monolayer with at least two zones, e.g., a proliferative zone and a nonproliferative zone, that can act as in vitro intestinal models. The models are two-dimensional, thus facilitating rapid and facile imaging. Systems comprising the tissue constructs and methods of using the constructs to study the effects of pharmaceuticals, nutraceuticals, and metabolites on intestinal cells are also described.
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公开(公告)号：US12104149B2
公开(公告)日：2024-10-01
申请号：US17044083
申请日：2019-05-15
Inventor： Nancy L. Allbritton , Yuli Wang , Raehyun Kim , Peter Joseph Attayek
CPC classification number: C12M41/34 , C12M21/08 , C12M23/34 , C12M25/02 , C12M41/46 , C12N5/0679 , C12N2500/02
Abstract: Disclosed herein are devices, apparatuses and methods for generating self-sustaining gaseous and non-gaseous gradients across a cell support structure and for culturing one or more cells or tissues under hypoxic conditions. Methods of generating one or more gas gradients across a cell support structure include providing a luminal container having a bottom wall that is a gas permeable membrane, positioning a cell support structure above the bottom wall, positioning one or more cells or tissues on the cell support structure, and generating one or more gas gradients between the bottom wall, across the cell support structure and into a luminal reservoir. An apparatus to produce hypoxic conditions for cell cultures includes a luminal container having a bottom wall, a cell support structure on the bottom wall, and a cover that sealably closes the open top, such that a hypoxic condition can be generated in the luminal reservoir.
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6.发明公开公开(公告)号：US20240010960A1
公开(公告)日：2024-01-11
申请号：US18371636
申请日：2023-09-22
Inventor： Nancy L. Allbritton , Yuli Wang , Raehyun Kim
Abstract: A method for producing tissue constructs comprising two or more distinct regions, each of which comprises a different cell population or lineage is described. The method involves providing a support substrate or substrate assembly comprising two or more physically distinct regions, wherein the two or more physically distinct regions of the support substrate or substrate assembly are different from each other; and depositing/positioning one or more cells on the support substrate or substrate assembly. The cells can form a continuous monolayer with at least two zones, e.g., a proliferative zone and a nonproliferative zone, that can act as in vitro intestinal models. The models are two-dimensional, thus facilitating rapid and facile imaging. Systems comprising the tissue constructs and methods of using the constructs to study the effects of pharmaceuticals, nutraceuticals, and metabolites on intestinal cells are also described.
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