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公开(公告)号：US20200231917A1
公开(公告)日：2020-07-23
申请号：US16525191
申请日：2019-07-29
Inventor： Nancy L. Allbritton , Yuli Wang , Christopher Sims
Abstract: A microfabricated device having at least one gas-entrapping feature formed therein in a configuration that entraps air bubbles upon wetting the feature with a solvent or solution is described. The device includes a sacrificial residue in contact with the gas-entrapping feature, the dissolution of which guides the wetting of the gas-entrapping feature.
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公开(公告)号：US20180223237A1
公开(公告)日：2018-08-09
申请号：US15945294
申请日：2018-04-04
Inventor： Nancy Allbritton , Christopher Sims , Yuli Wang , Pavak Kirit Shah
CPC classification number: C12M23/12 , B01J19/0046 , B01J2219/00452 , B01J2219/00743 , C12M23/20 , C12M23/22 , C12M25/04 , C12M25/16 , C12M47/02 , C12M47/04
Abstract: An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).
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3.发明申请公开(公告)号：US20180217494A1
公开(公告)日：2018-08-02
申请号：US15738179
申请日：2016-06-23
Inventor： Anne Taylor , Joyce Kamande , Yuli Wang
CPC classification number: G03F7/0002 , B29C39/006 , B29L2031/756 , G03F7/0017 , G03F2009/005
Abstract: A method for pattern transfer to a silicone-based microstructure device comprises the steps of molding a silicone-based negative replica from a lithography patterned master mold. An epoxy resin-based master mold is molded from the silicone-based replica. A surface of the epoxy resin-based master mold is coated with a layer of Cr and then with a layer of Au on the CR layer to facilitate demolding of a silicone-based material. The silicone-based microstructure device is then molded from the coated epoxy resin-based master mold, wherein the silicone-based microstructure device has a dimensional pattern that substantially corresponds to the dimensional pattern of the lithography patterned master mold.
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公开(公告)号：US09994805B2
公开(公告)日：2018-06-12
申请号：US14404225
申请日：2013-05-31
Inventor： Nancy Allbritton , Yuli Wang , Christopher Sims
CPC classification number: C12M23/16 , B01L3/5027 , B01L3/502707 , B01L2200/0684 , B01L2200/12 , B01L2300/161 , B81B2201/058 , B81C1/00206 , C12M23/12 , C12M29/20 , C12Q1/02 , G01N2035/00158
Abstract: A microfabricated device having at least one gas-entrapping feature formed therein in a configuration that entraps air bubbles upon wetting the feature with a solvent or solution is described. The device includes a sacrificial residue in contact with the gas-entrapping feature, the dissolution of which guides the wetting of the gas-entrapping feature.
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公开(公告)号：US09765291B2
公开(公告)日：2017-09-19
申请号：US14714620
申请日：2015-05-18
Inventor： Nancy Allbritton , Christopher Sims , Yuli Wang , Pavak Kirit Shah
CPC classification number: C12M23/12 , B01J19/0046 , B01J2219/00452 , B01J2219/00743 , C12M23/20 , C12M23/22 , C12M25/04 , C12M25/16 , C12M47/02 , C12M47/04
Abstract: An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).
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Patent Agency Ranking
公开(公告)号：US20250027027A1
公开(公告)日：2025-01-23
申请号：US18786858
申请日：2024-07-29
Inventor： Nancy L. Allbritton , Yuli Wang , Raehyun Kim , Peter Joseph Attayek
Abstract: Disclosed herein are devices, apparatuses and methods for generating self-sustaining gaseous and non-gaseous gradients across a cell support structure and for culturing one or more cells or tissues under hypoxic conditions. Methods of generating one or more gas gradients across a cell support structure include providing a luminal container having a bottom wall that is a gas permeable membrane, positioning a cell support structure above the bottom wall, positioning one or more cells or tissues on the cell support structure, and generating one or more gas gradients between the bottom wall, across the cell support structure and into a luminal reservoir. An apparatus to produce hypoxic conditions for cell cultures includes a luminal container having a bottom wall, a cell support structure on the bottom wall, and a cover that sealably closes the open top, such that a hypoxic condition can be generated in the luminal reservoir.
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公开(公告)号：US12195706B2
公开(公告)日：2025-01-14
申请号：US18371636
申请日：2023-09-22
Inventor： Nancy L. Allbritton , Yuli Wang , Raehyun Kim
Abstract: A method for producing tissue constructs comprising two or more distinct regions, each of which comprises a different cell population or lineage is described. The method involves providing a support substrate or substrate assembly comprising two or more physically distinct regions, wherein the two or more physically distinct regions of the support substrate or substrate assembly are different from each other; and depositing/positioning one or more cells on the support substrate or substrate assembly. The cells can form a continuous monolayer with at least two zones, e.g., a proliferative zone and a nonproliferative zone, that can act as in vitro intestinal models. The models are two-dimensional, thus facilitating rapid and facile imaging. Systems comprising the tissue constructs and methods of using the constructs to study the effects of pharmaceuticals, nutraceuticals, and metabolites on intestinal cells are also described.
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公开(公告)号：US12104149B2
公开(公告)日：2024-10-01
申请号：US17044083
申请日：2019-05-15
Inventor： Nancy L. Allbritton , Yuli Wang , Raehyun Kim , Peter Joseph Attayek
CPC classification number: C12M41/34 , C12M21/08 , C12M23/34 , C12M25/02 , C12M41/46 , C12N5/0679 , C12N2500/02
Abstract: Disclosed herein are devices, apparatuses and methods for generating self-sustaining gaseous and non-gaseous gradients across a cell support structure and for culturing one or more cells or tissues under hypoxic conditions. Methods of generating one or more gas gradients across a cell support structure include providing a luminal container having a bottom wall that is a gas permeable membrane, positioning a cell support structure above the bottom wall, positioning one or more cells or tissues on the cell support structure, and generating one or more gas gradients between the bottom wall, across the cell support structure and into a luminal reservoir. An apparatus to produce hypoxic conditions for cell cultures includes a luminal container having a bottom wall, a cell support structure on the bottom wall, and a cover that sealably closes the open top, such that a hypoxic condition can be generated in the luminal reservoir.
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公开(公告)号：US11926808B2
公开(公告)日：2024-03-12
申请号：US17361039
申请日：2021-06-28
Inventor： Nancy L. Allbritton , Christopher E. Sims , Yuli Wang , Pavak Kirit Shah
CPC classification number: C12M23/12 , B01J19/0046 , C12M23/20 , C12M23/22 , C12M25/04 , C12M25/16 , C12M47/02 , C12M47/04 , C12N1/02 , C12N5/0068 , B01J2219/00452 , B01J2219/00743 , C12N2509/10
Abstract: An apparatus for collecting or culturing cells or cell colonies includes: a common substrate formed from a flexible resilient polymeric material and having a plurality of wells formed therein; and a plurality of rigid cell carriers releasably connected to said common substrate, with said carriers arranged in the form of an array, and with each of the carriers resiliently received in one of the wells. A method of collecting or culturing cells or cell colonies with such an apparatus is carried out by depositing a liquid media carrying cells on the apparatus so that said cells settle on or adhere to said the carriers; and then (c) releasing at least one selected carrier having said cells thereon by gradual application of release energy to each carrier from the cavity in which it is received (e.g., by pushing with a probe).
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10.发明公开公开(公告)号：US20240010960A1
公开(公告)日：2024-01-11
申请号：US18371636
申请日：2023-09-22
Inventor： Nancy L. Allbritton , Yuli Wang , Raehyun Kim
Abstract: A method for producing tissue constructs comprising two or more distinct regions, each of which comprises a different cell population or lineage is described. The method involves providing a support substrate or substrate assembly comprising two or more physically distinct regions, wherein the two or more physically distinct regions of the support substrate or substrate assembly are different from each other; and depositing/positioning one or more cells on the support substrate or substrate assembly. The cells can form a continuous monolayer with at least two zones, e.g., a proliferative zone and a nonproliferative zone, that can act as in vitro intestinal models. The models are two-dimensional, thus facilitating rapid and facile imaging. Systems comprising the tissue constructs and methods of using the constructs to study the effects of pharmaceuticals, nutraceuticals, and metabolites on intestinal cells are also described.
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