公开(公告)号：US11578132B2

公开(公告)日：2023-02-14

申请号：US16478295

申请日：2018-01-15

Applicant: KANEKA CORPORATION ,  TOKYO UNIVERSITY OF SCIENCE FOUNDATION

Inventor： Tatsuya Moutai ,  Tomoyuki Nakaishi ,  Hiroshi Kita ,  Mitsuaki Kitano ,  Daisuke Kitamura

IPC: C12N15/85 ,  C07K16/28 ,  C12N5/0781 ,  A61K39/00

Abstract: It is an object of the present invention to provide a method for efficiently producing a B cell population comprising B cells that recognize a specific antigen. According to the present invention, provided is a method for producing a B cell population, comprising: a step (c) of culturing a cell population comprising B cells together with a specific antigen in the absence of IL-21, in the absence of IL-4, and in the presence of a cytokine other than IL-21 and IL-4, while giving stimulation mediated by CD40 and a BAFF receptor to the cells; and a step (d) of culturing the cell population comprising B cells, while giving stimulation mediated by has to the cells, so as to obtain a B cell population comprising B cells that recognize the specific antigen.

公开(公告)号：US20200071410A1

公开(公告)日：2020-03-05

申请号：US16478295

申请日：2018-01-15

Applicant: KANEKA CORPORATION ,  TOKYO UNIVERSITY OF SCIENCE FOUNDATION

Inventor： Tatsuya Moutai ,  Tomoyuki Nakaishi ,  Hiroshi Kita ,  Mitsuaki Kitano ,  Daisuke Kitamura

IPC: C07K16/28 ,  C12N5/0781

Abstract: It is an object of the present invention to provide a method for efficiently producing a B cell population comprising B cells that recognize a specific antigen. According to the present invention, provided is a method for producing a B cell population, comprising: a step (c) of culturing a cell population comprising B cells together with a specific antigen in the absence of IL-21, in the absence of IL-4, and in the presence of a cytokine other than IL-21 and IL-4, while giving stimulation mediated by CD40 and a BAFF receptor to the cells; and a step (d) of culturing the cell population comprising B cells, while giving stimulation mediated by has to the cells, so as to obtain a B cell population comprising B cells that recognize the specific antigen.

公开(公告)号：US10781423B2

公开(公告)日：2020-09-22

申请号：US16180592

申请日：2018-11-05

Applicant: TOKYO UNIVERSITY OF SCIENCE FOUNDATION ,  KANEKA CORPORATION

Inventor： Daisuke Kitamura ,  Tomoyuki Nakaishi

IPC: C12N5/00 ,  C12N5/02 ,  A61K38/00 ,  C12N5/0781 ,  C12N15/09 ,  C12N5/10 ,  C12N15/86 ,  C07K16/00 ,  C07K14/47 ,  C12N7/00

Abstract: It is an object of the present invention to provide a method for easily producing an antigen-specific B cell population comprising IgG-positive B cells specific to a specific antigen. The present invention provides a method for producing a B cell population, comprising culturing B cells, in which the expression of a Bach2 gene has been increased, in the presence of a means for acting on CD40 and/or a BAFF receptor.

公开(公告)号：US20200255802A1

公开(公告)日：2020-08-13

申请号：US16180592

申请日：2018-11-05

Applicant: TOKYO UNIVERSITY OF SCIENCE FOUNDATION ,  KANEKA CORPORATION

Inventor： Daisuke Kitamura ,  Tomoyuki Nakaishi

IPC: C12N5/0781 ,  C12N15/09 ,  C12N5/10 ,  C12N15/86 ,  C12N7/00 ,  C07K16/00 ,  C07K14/47

Abstract: It is an object of the present invention to provide a method for easily producing an antigen-specific B cell population comprising IgG-positive B cells specific to a specific antigen. The present invention provides a method for producing a B cell population, comprising culturing B cells, in which the expression of a Bach2 gene has been increased, in the presence of a means for acting on CD40 and/or a BAFF receptor.

公开(公告)号：US10428304B2

公开(公告)日：2019-10-01

申请号：US15322325

申请日：2015-06-30

Applicant: TOKYO UNIVERSITY OF SCIENCE FOUNDATION ,  KANEKA CORPORATION

Inventor： Daisuke Kitamura ,  Tomoyuki Nakaishi

IPC: C12N5/00 ,  C12N5/02 ,  A61K38/00 ,  C12N5/0781 ,  C12N5/10 ,  C12N15/09 ,  C07K14/47 ,  C07K16/00 ,  C12N7/00 ,  C12N15/86

Abstract: It is an object of the present invention to provide a method for easily producing an antigen-specific B cell population comprising IgG-positive B cells specific to a specific antigen. The present invention provides a method for producing a B cell population, comprising culturing B cells, in which the expression of a Bach2 gene has been increased, in the presence of a means for acting on CD40 and/or a BAFF receptor.