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公开(公告)号：US20090209003A1
公开(公告)日：2009-08-20
申请号：US11994437
申请日：2006-07-04
申请人： Tadaaki Tokida , Satoshi Hihara , Takashi Kudou , Akira Kawamura , Hirofumi Doi , Yoshizumi Ishino
发明人： Tadaaki Tokida , Satoshi Hihara , Takashi Kudou , Akira Kawamura , Hirofumi Doi , Yoshizumi Ishino
CPC分类号： C07K14/4738 , C12N9/1252 , C12N15/69
摘要： The present invention is to construct a DNA replication reaction system which is excellent in versatility and is easily used. An amino acid sequence of a PCNA monomer which is one of factors involved in DNA replication is prepared so that amino acid residues causing mutual charge repulsion constitute a site which causes, when an N terminal region of the PCNA monomer and a C terminal region of another PCNA monomer act as an interface to form a multimeric complex, an intermolecular interaction of the monomers in an interface region of the monomers.
摘要翻译： 本发明是构建通用性优异且易于使用的DNA复制反应体系。 制备作为涉及DNA复制的因素之一的PCNA单体的氨基酸序列，使得导致相互电荷排斥的氨基酸残基构成了当PCNA单体的N末端区域和另一个的C末端区域时引起的位点 PCNA单体作为形成多聚体复合物的界面，单体在界面区域中的分子间相互作用。
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公开(公告)号：US08003346B2
公开(公告)日：2011-08-23
申请号：US11994437
申请日：2006-07-04
申请人： Tadaaki Tokida , Satoshi Hihara , Takashi Kudou , Akira Kawamura , Hirofumi Doi , Yoshizumi Ishino
发明人： Tadaaki Tokida , Satoshi Hihara , Takashi Kudou , Akira Kawamura , Hirofumi Doi , Yoshizumi Ishino
CPC分类号： C07K14/4738 , C12N9/1252 , C12N15/69
摘要： The present invention is to construct a DNA replication reaction system which is excellent in versatility and is easily used. An amino acid sequence of a PCNA monomer which is one of factors involved in DNA replication is prepared so that amino acid residues causing mutual charge repulsion constitute a site which causes, when an N terminal region of the PCNA monomer and a C terminal region of another PCNA monomer act as an interface to form a multimeric complex, an intermolecular interaction of the monomers in an interface region of the monomers.
摘要翻译： 本发明是构建通用性优异且易于使用的DNA复制反应体系。 制备作为涉及DNA复制的因素之一的PCNA单体的氨基酸序列，使得导致相互电荷排斥的氨基酸残基构成了当PCNA单体的N末端区域和另一个的C末端区域时引起的位点 PCNA单体作为形成多聚体复合物的界面，单体在界面区域中的分子间相互作用。
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3.发明申请Interaction inhibitors, method of detecting interaction inhibitor and kit detecting interaction inhibitor 审中-公开相互作用抑制剂，相互作用抑制剂检测方法和试剂盒检测相互作用抑制剂公开(公告)号：US20070134663A1
公开(公告)日：2007-06-14
申请号：US10568578
申请日：2004-08-13
申请人： Satoshi Hihara , Hirofumi Doi
发明人： Satoshi Hihara , Hirofumi Doi
CPC分类号： G01N33/6875 , C12Q1/485 , G01N2500/02
摘要： The task is to provide protein interaction inhibitors and/or detection method for interaction inhibitors related to regulation of T cell activation and production of IL-2. The present invention provides inhibitors of interaction between PKC theta and KPNA1, and inhibitors of interaction between KPNA1 and NF-kappaB. Inhibitors of interaction is defined as an candidate compound demonstrating the inhibition of interaction by assaying whether interaction has occurred or not between 2 types of protein and the candidate compound under the conditions allowing interaction of each combination. The detection kit is equipped with samples providing proteins of combinations to be interacted.
摘要翻译： 任务是提供蛋白质相互作用抑制剂和/或与调节T细胞活化和产生IL-2相关的相互作用抑制剂的检测方法。 本发明提供PKCθ与KPNA1之间相互作用的抑制剂，以及KPNA1与NF-κB之间相互作用的抑制剂。 相互作用的抑制剂被定义为通过测定在允许每种组合的相互作用的条件下，在两种类型的蛋白质和候选化合物之间是否发生相互作用来证明相互作用的抑制的候选化合物。 检测试剂盒配备提供待相互作用的组合蛋白质的样品。
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