公开(公告)号：US20090004227A1

公开(公告)日：2009-01-01

申请号：US11628535

申请日：2005-06-07

申请人： Takeshi Matsumura ,  Akira Ito ,  Shigeki Kobayashi ,  Katsumi Kume ,  Keizo Ito ,  Noriko Itchoda ,  Chihiro Sugimoto

发明人： Takeshi Matsumura ,  Akira Ito ,  Shigeki Kobayashi ,  Katsumi Kume ,  Keizo Ito ,  Noriko Itchoda ,  Chihiro Sugimoto

IPC分类号： A61K39/00 ,  A01H5/00 ,  C07H21/04 ,  A61P31/00

CPC分类号： A61K36/81 ,  A61K38/00 ,  A61K2039/517 ,  A61K2039/542 ,  C12N15/8258 ,  C12N2770/16022

摘要： The present invention provides a system in which a recombinant plant expressing a target peptide is orally administered to thereby achieve exertion of the function of the peptide, along with the genetically recombinant plant, and the present invention relates to a carrier system for oral administration of a target peptide by oral administration of a plant (potato) that expresses a target peptide by expressing for example a fused gene obtained by fusing the LRE1 gene coding for the immunogenic peptide LRE1 derived from the Leucocytozoon caulleryi 2nd-generation schizont with a Norwalk virus coat protein gene, along with the relevant genetically recombinant plant, and thus, in the present invention the orally administered target peptide can reach an intended destination (such as the intestinal mucosa) without being decomposed in the digestive tract, so that the function of the target peptide can be induced.

摘要翻译： 本发明提供了一种系统，其中口服施用表达目标肽的重组植物，从而与遗传重组植物一起实现肽的功能的运用，本发明涉及一种口服给药的载体体系 通过口服表达目标肽的植物（马铃薯），通过表达例如通过将编码来源于白细胞介素2代裂殖体的免疫原性肽LRE1的LRE1基因与诺瓦克病毒外壳蛋白融合获得的融合基因， 基因以及相关的遗传重组植物，因此在本发明中，口服给药的目标肽可以在消化道中不分解而达到预期的目的地（例如肠粘膜），从而靶肽的功能 可以诱导。

公开(公告)号：US08617575B2

公开(公告)日：2013-12-31

申请号：US11597879

申请日：2005-05-30

申请人： Akira Ito ,  Toru Gotanda ,  Shigeki Kobayashi ,  Katsumi Kume ,  Takeshi Matsumura

发明人： Akira Ito ,  Toru Gotanda ,  Shigeki Kobayashi ,  Katsumi Kume ,  Takeshi Matsumura

IPC分类号： A61K39/00 ,  A61K39/002 ,  C12N1/20 ,  C12N15/00 ,  C12N15/82 ,  C12N15/30 ,  C07K14/00 ,  A01H5/00

CPC分类号： C07K14/44 ,  A61K39/015 ,  A61K2039/542 ,  C12N15/8258

摘要： Immunity against protozoan is conferred on an animal by a method comprising orally administering to an animal a transformed plant cell comprising a polynucleotide encoding a protective antigen against protozoiasis development, a transformed plant or its progeny or clone comprising the transformed cell, a propagation material of the plant or its progeny or clone, a processed material or extract of the above cell, plant, or its progeny or clone, or propagation material, or a protective antigen against protozoiasis development isolated from the transformed plant cell or the transformed plant or its progeny or clone.

摘要翻译： 通过包括向动物口服施用包含编码抗原虫病发展的保护性抗原的多核苷酸的转化植物细胞，包含转化细胞的转化植物或其后代或克隆，包含转化细胞的繁殖材料的方法赋予动物免疫力 植物或其后代或克隆，上述细胞，植物或其后代或克隆或繁殖材料的处理材料或提取物，或抗原原生动物发育的保护性抗原，其从转化的植物细胞或转化的植物或其后代分离，或 克隆。

公开(公告)号：US20080003243A1

公开(公告)日：2008-01-03

申请号：US11597879

申请日：2005-05-30

申请人： Akira Ito ,  Toru Gotanda ,  Shigeki Kobayashi ,  Katsumi Kume ,  Takeshi Matsumura

发明人： Akira Ito ,  Toru Gotanda ,  Shigeki Kobayashi ,  Katsumi Kume ,  Takeshi Matsumura

IPC分类号： A61K39/00 ,  A01H5/00 ,  A61P43/00 ,  C12N15/00 ,  C12N15/82

CPC分类号： C07K14/44 ,  A61K39/015 ,  A61K2039/542 ,  C12N15/8258

摘要： Leaves of potato plants transformed with a vector that expresses an R7 immunogenic protein gene derived from second-generation schizonts of Leucocytozoon caulleryiwere administered to chickens as an oral vaccine. As a result, the present inventors succeeded in increasing the antibody titer of the chickens.

摘要翻译： 用表达来自白细胞介素的第二代裂殖体的R7免疫原性蛋白质基因的载体转化的马铃薯植物的叶子作为口服疫苗施用于鸡。 结果，本发明人成功地提高了鸡的抗体滴度。

公开(公告)号：US4770875A

公开(公告)日：1988-09-13

申请号：US119075

申请日：1987-11-10

申请人： Katsumi Kume ,  Toyotsugu Nakai ,  Hiroshi Nishizawa ,  Takashi Yoshikawa ,  Hirofumi Danbara

发明人： Katsumi Kume ,  Toyotsugu Nakai ,  Hiroshi Nishizawa ,  Takashi Yoshikawa ,  Hirofumi Danbara

IPC分类号： C12N15/09 ,  A61K39/10 ,  C12N1/20 ,  C12N15/00 ,  C12R1/01 ,  C12N1/00

CPC分类号： A61K39/099 ,  Y10S424/825 ,  Y10S424/83

摘要： A process for the preparation of a mutant strain of B. bronchiseptica having chromosomal genetic markers carrying at least temperature-sensitive (grown at 42.degree. C.), nalidixic acid-resistant and heat-labile dermonecrotic toxin producing ability negative markers, suitable for the preparation of live attenuated AR vaccine, which comprises subjecting a strain of B. bronchiseptica to an induced mutation, isolating a mutant having the genetic markers of being at least temperature-sensitive (grown at 42.degree. C.), nalidixic acid-resistant and heat-labile dermonecrotic toxin negative, culturing the isolated strain of B. bronchiseptica on a Bordet-gengou agar plate containing nalidixic acid at 42.degree. C., thereafter picking up an organism morphologically resembling a phase-I organism, repeating the procedures of culturing and picking up, and converting the organisms to a heat-labile dermonecrotic toxin-producing ability negative phase-I organism having a complete capsular antigen.

摘要翻译： 一种制备支气管炎支原体突变菌株的方法，该菌株具有至少具有温度敏感性（42℃生长），萘啶酸耐受性和热不稳定性皮肤坏死毒素生成能力阴性标记的染色体遗传标记，适用于 活的减毒AR疫苗的制备，其包括使支气管炎支原体菌株进行诱导突变，分离具有至少温度敏感性（在42℃生长）的遗传标记的突变体，耐萘啶酸和热 可溶性皮肤坏死毒素阴性，在含有萘啶酸的Bordet-gengou琼脂平板上培养支气管炎支原体的分离菌株，然后取出形态上类似于I期生物体的生物，重复培养和采摘的程序 并将生物体转化为具有完全荚膜抗原的热不稳定的皮肤坏死毒素生产能力的负相I生物体。