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公开(公告)号：US07189552B2
公开(公告)日：2007-03-13
申请号：US10539396
申请日：2003-12-16
申请人： Tang Lan , Wu Wenping , Junxin Duan , Pia Francke Johannesen
发明人： Tang Lan , Wu Wenping , Junxin Duan , Pia Francke Johannesen
CPC分类号： C12N9/242 , A21D8/042 , C12C11/003 , C12N9/2414 , C12P7/02 , C12P19/12 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要： An isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO:4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO:3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
摘要翻译： 一种分离的多核苷酸，其包含编码具有α-淀粉酶活性的多肽的开放阅读框，所述多肽选自：a）包含氨基酸序列的多肽，所述氨基酸序列与SEQ ID NO：22至450的氨基酸具有至少70％的同一性 编号：4; b）一种多肽，其包含氨基酸序列，该氨基酸序列与插入存在于根据布达佩斯条约与DSMZ保藏的大肠杆菌宿主的质粒存在的质粒的多核苷酸编码的多肽编码的多肽相同， 15334; c）由多核苷酸编码的多肽，其包含与SEQ ID NO：3中的位置68至1417所示的序列具有至少70％同一性的核苷酸序列; 和d）具有α-淀粉酶活性的（a），（b）或（c）的片段。
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公开(公告)号：US20120003700A1
公开(公告)日：2012-01-05
申请号：US13230984
申请日：2011-09-13
申请人： Tang Lan , Wu Wenping , Junxin Duan , Pia Francke Johannesen
发明人： Tang Lan , Wu Wenping , Junxin Duan , Pia Francke Johannesen
CPC分类号： C12N9/242 , A21D8/042 , C12C11/003 , C12N9/2414 , C12P7/02 , C12P19/12 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要： The present invention relates to an isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO: 4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO: 3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
摘要翻译： 本发明涉及一种分离的多核苷酸，其包含编码具有α-淀粉酶活性的多肽的开放阅读框，所述多肽选自：a）包含与氨基酸具有至少70％同一性的氨基酸序列的多肽 SEQ ID NO：4的22至450; b）一种多肽，其包含氨基酸序列，该氨基酸序列与插入存在于根据布达佩斯条约与DSMZ保藏的大肠杆菌宿主的质粒存在的质粒的多核苷酸编码的多肽编码的多肽相同， 15334; c）由多核苷酸编码的多肽，其包含与SEQ ID NO：3中的位置68至1417所示的序列具有至少70％同一性的核苷酸序列; 和d）具有α-淀粉酶活性的（a），（b）或（c）的片段。
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公开(公告)号：US20060051849A1
公开(公告)日：2006-03-09
申请号：US10539396
申请日：2003-12-16
申请人： Tang Lan , Wu Wenping , Junxin Duan , Pia Johannesen
发明人： Tang Lan , Wu Wenping , Junxin Duan , Pia Johannesen
CPC分类号： C12N9/242 , A21D8/042 , C12C11/003 , C12N9/2414 , C12P7/02 , C12P19/12 , C12P19/14 , C12Y302/01001 , D06M16/003 , Y02E50/17
摘要： An isolated polynucleotide comprising an open reading frame encoding a polypeptide having alpha-amylase activity, the polypeptide selected from the group consisting of: a) a polypeptide comprising an amino acid sequence which has at least 70% identity with amino acids 22 to 450 of SEQ ID NO:4; b) a polypeptide comprising an amino acid sequence which has at least 70% identity with the polypeptide encoded by the amylase encoding part of the polynucleotide inserted into a plasmid present in the E. coli host deposited under the Budapest Treaty with DSMZ under accession number DSM 15334; c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence which has at least 70% identity with the sequence shown from position 68 to 1417 in SEQ ID NO:3; and d) a fragment of (a), (b) or (c) that has alpha-amylase activity.
摘要翻译： 一种分离的多核苷酸，其包含编码具有α-淀粉酶活性的多肽的开放阅读框，所述多肽选自：a）包含氨基酸序列的多肽，所述氨基酸序列与SEQ ID NO：22至450的氨基酸具有至少70％的同一性 编号：4; b）一种多肽，其包含氨基酸序列，该氨基酸序列与插入存在于根据布达佩斯条约与DSMZ保藏的大肠杆菌宿主的质粒存在的质粒的多核苷酸编码的多肽编码的多肽相同， 15334; c）由多核苷酸编码的多肽，其包含与SEQ ID NO：3中的位置68至1417所示的序列具有至少70％同一性的核苷酸序列; 和d）具有α-淀粉酶活性的（a），（b）或（c）的片段。
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公开(公告)号：US20070166432A1
公开(公告)日：2007-07-19
申请号：US10585620
申请日：2004-12-22
申请人： Ye Liu , Tang Lan , Tina Spendler , Mary Stringer
发明人： Ye Liu , Tang Lan , Tina Spendler , Mary Stringer
摘要： The inventors have identified amylases in fungal strains of Valsaria and found that the amylase can increase the shelf life of baked products. Particularly, the novel amylase in com-bination with a maltogenic amylase further improves the softness of bread crumb without hav-ing detrimental effects on elasticity.
摘要翻译： 发明人鉴定了Valsaria真菌菌株中的淀粉酶，发现淀粉酶可以提高焙烤产品的保质期。 特别地，与麦芽糖淀粉酶组合的新型淀粉酶进一步改善了面包屑的柔软性，而不会对弹性造成不利影响。
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公开(公告)号：US07666650B2
公开(公告)日：2010-02-23
申请号：US10585620
申请日：2004-12-22
申请人： Liu Ye , Tang Lan , Tina Spendler , Mary Ann Stringer
发明人： Liu Ye , Tang Lan , Tina Spendler , Mary Ann Stringer
摘要： The inventors have identified amylases in fungal strains of Valsaria and found that the amylase can increase the shelf life of baked products. Particularly, the novel amylase in combination with a maltogenic amylase further improves the softness of bread crumb without having detrimental effects on elasticity.
摘要翻译： 发明人鉴定了Valsaria真菌菌株中的淀粉酶，发现淀粉酶可以提高焙烤产品的保质期。 特别地，与麦芽糖淀粉酶组合的新型淀粉酶进一步提高了面包屑的柔软性，而不会对弹性产生不利影响。
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