公开(公告)号：US20160016170A1

公开(公告)日：2016-01-21

申请号：US14335027

申请日：2014-07-18

Applicant: Tecan Trading AG

Inventor： Tiffany LAY ,  Daniel HOFFMEYER ,  Anne R. KOPF-SILL

IPC: B01L3/00 ,  G01N1/10

CPC classification number: B01L3/502792 ,  B01L3/0275 ,  B01L3/50273 ,  B01L2200/027 ,  B01L2300/0816 ,  B01L2300/161 ,  B01L2400/0427

Abstract: A disposable cartridge configured as a digital microfluidics system for manipulating samples in liquid portions having a cartridge accommodation site and a central control unit for controlling selection of individual electrodes of an electrode array located at the site and for providing plural electrodes with individual voltage pulses for manipulating liquid portions by electrowetting. The cartridge has a hydrophobic working surface and a rigid cover with a second hydrophobic surface, the hydrophobic surfaces facing each other and being separated in parallel planes by a gap. The cartridge has plural pipetting guides for safe entering/withdrawing liquids into/from the gap with a pipette tip. At least one of the pipetting guides provides an abutting surface sealingly admittable by a counter surface of a pipette tip, located at a pipetting orifice that reaches through the rigid cover, and configured to prevent a pipette tip from touching the hydrophobic working surface.

Abstract translation: 一种被配置为数字微流体系统的一次性盒，用于在具有盒容纳位置的液体部分中操作样品，并且控制中央控制单元，用于控制位于该位置处的电极阵列的各个电极的选择，并且为多个电极提供用于操纵的各个电压脉冲 液体部分通过电润湿。 所述筒具有疏水性工作表面和具有第二疏水性表面的刚性盖，所述疏水性表面彼此相对并且在平行的平面上被间隔开。 药筒具有多个移液引导件，用于以移液管吸头将液体安全地进入/从间隙排出。 移液引导件中的至少一个提供了通过移液管尖端的相对表面密封地附着的邻接表面，该移液管尖端位于移动孔口处，该移液孔口通过刚性覆盖物，并且构造成防止移液管尖端接触疏水性工作表面。