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公开(公告)号:US20150184225A1
公开(公告)日:2015-07-02
申请号:US14409992
申请日:2013-06-25
发明人: Pak Kin Wong , Yi Lu , Tingting Liu , Vincent Gau
CPC分类号: C12Q1/686 , B01L3/502761 , B01L7/525 , B01L2200/0668 , B01L2200/10 , B01L2300/0645 , B01L2300/1833 , B01L2400/0415 , B01L2400/0424 , B01L2400/0445 , C12Q2523/307 , C12Q2561/113 , C12Q2565/607
摘要: Described herein are microfluidic diagnostic methods and devices using electrokinetic modules for the isolation of targets (e.g., cells, bacteria, biomolecules) from biological samples, PCR amplification of DNA isolated from the targets, and real-time quantification of the amplified DNA using impedance sensing. Sample preparation, PCR amplification, and impedance sensing are thus performed using a single integrated platform.
摘要翻译: 本文描述了使用电动模块用于从生物样品分离靶(例如细胞,细菌,生物分子)的微流体诊断方法和装置,从靶标分离的DNA的PCR扩增,以及使用阻抗感测实时定量扩增的DNA 。 因此,使用单个集成平台执行样品制备,PCR扩增和阻抗感测。