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    Asymmetric benzoxanthene dye labelling reagents
    2.
    发明申请
    Asymmetric benzoxanthene dye labelling reagents 有权
    不对称苯并氧杂蒽染料标记试剂

    公开(公告)号：US20020115067A1

    公开(公告)日：2002-08-22

    申请号：US09976842

    申请日：2001-10-11

    Applicant: The Perkin-Elmer Corporation

    Inventor： Scott C. Benson ,  Steven M. Menchen ,  Peter D. Theisen ,  Kevin M. Hennessey ,  Vergine C. Furniss ,  Joan D. Hauser

    IPC: C12Q001/70 ,  C12Q001/68 ,  C07H021/04 ,  C07D491/04 ,  C07D311/78

    CPC classification number: C07H21/00 ,  C07C39/38 ,  C09B11/00 ,  C09B11/08 ,  C12Q1/6869

    Abstract: A class of asymmetric monobenzoxanthene compounds useful as fluorescent dyes are disclosed having the structure 1 wherein Y1 and Y2 are individually hydroxyl amino, imminium, or oxygen, R1-r8 are hydrogen, fluorine, chlorine, alkyl, alkene, alkyne, sulfonate, amino, amido, nitrile, alkoxy, linking group, and combinations thereof and R9 is acetylene, alkane, alkene, cyano, substituted phenyl, and combinations thereof. The invention further includes novel intermediate compounds useful for the synthesis of asymmetric benzoxanthene compounds having the general structure 2 where substituents R3-R7 correspond to like-referenced substituents in the structure of described above, and Y2 is hydroxyl or amine. In another aspect the invention includes methods for synthesizing the above dye compounds and intermediates. In yet another aspect the present invention includes reagents labeled with the asymmetric benzoxanthene dye compounds, including deoxynucleotides, dideoxynucleotides, phosphoramidites, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    Abstract translation: 公开了一类用作荧光染料的不对称单苯并氧蒽化合物，其结构是其中Y 1和Y 2分别是羟基氨基，亚氨基或氧，R 1 -R 8是氢，氟，氯，烷基，烯烃，炔烃，磺酸酯，氨基，酰氨基 腈，烷氧基，连接基团及其组合，R 9为乙炔，烷烃，烯烃，氰基，取代的苯基及其组合。 本发明还包括可用于合成具有通式结构的不对称苯并氧杂蒽化合物的新型中间体化合物，其中取代基R 3〜R 7对应于上述结构中的相同取代基，Y 2为羟基或胺。 另一方面，本发明包括合成上述染料化合物和中间体的方法。 在另一方面，本发明包括用不对称苯并氧杂蒽染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸，亚磷酰胺和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

    Propargylethoxyamino nucleotide primer extensions
    3.
    发明申请
    Propargylethoxyamino nucleotide primer extensions 有权
    丙炔乙氧基氨基核苷酸引物延伸

    公开(公告)号：US20020045180A1

    公开(公告)日：2002-04-18

    申请号：US09881898

    申请日：2001-06-14

    Applicant: The Perkin-Elmer Corporation

    Inventor： Shaheer H. Khan ,  Steven M. Menchen ,  Barnett B. Rosenblum

    IPC: C12Q001/68 ,  C07H019/16 ,  C07H019/048

    CPC classification number: C07H21/00 ,  C07H19/04 ,  C07H21/04 ,  C12Q1/686

    Abstract: Propargylethoxyamino nucleosides are disclosed having the structure 1 wherein R1 and R2 are nullH, lower alkyl, or label; B is a 7-deazapurine, purine, or pyrimidine nucleoside base; W1 is nullH or nullOH; W2 is nullOH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3null-position; and W3 is nullPO4, nullP2O7, nullP3O10, phosphate analog, or nullOH. Additionally, a primer extension method is provided employing the above propargylethoxyamino nucleosides.

    Abstract translation: 公开了具有其中R 1和R 2为-H，低级烷基或标记的结构的丙炔乙氧基氨基核苷; B是7-脱氮嘌呤，嘌呤或嘧啶核苷碱基; W1是-H或-OH; W2是-OH或使核苷不能在3'-位形成磷酸二酯键的部分; W3是-PO 4，-P 2 O 7，-P 3 O 10，磷酸酯类似物或-OH。 另外，使用上述炔丙氧基氨基核苷提供引物延伸方法

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