公开(公告)号：US20040142344A1

公开(公告)日：2004-07-22

申请号：US10648945

申请日：2003-08-26

Applicant: The Regents of the University of California

Inventor： Guillermo C. Bazan ,  Brent S. Gaylord ,  Shu Wang

IPC: C12Q001/68

CPC classification number: C12Q1/6834 ,  C12Q1/6813 ,  C12Q1/6818 ,  C12Q1/6825 ,  Y10T436/143333 ,  C12Q2565/107 ,  C12Q2563/107 ,  C12Q2523/313

Abstract: Methods, compositions and articles of manufacture for assaying a sample for a target polynucleotide are provided. A sample suspected of containing the target polynucleotide is contacted with a polycationic multichromophore and a sensor polynucleotide complementary to the target polynucleotide. The sensor polynucleotide comprises a signaling chromophore to receive energy from the excited multichromophore and increase emission in the presence of the target polynucleotide. The methods can be used in multiplex form. Kits comprising reagents for performing such methods are also provided.

Abstract translation: 提供了用于测定靶多核苷酸样品的方法，组合物和制品。 将涉及含有靶多核苷酸的样品与聚阳离子多重色素和与靶多核苷酸互补的传感器多核苷酸接触。 所述传感器多核苷酸包含用于从所述激发的多色素载体接收能量的信号发色团，并且在靶多核苷酸的存在下增加发射。 该方法可以多路复用形式使用。 还提供了包含用于执行这些方法的试剂的试剂盒。