公开(公告)号：US11504427B2

公开(公告)日：2022-11-22

申请号：US16463316

申请日：2017-11-22

Applicant: The Regents of the University of California

Inventor： Yingxiao Wang ,  Shu Chien ,  Yijia Pan ,  Yiqian Wu ,  Shaoying Lu ,  Kirk Shung

IPC: A61K41/00 ,  C07K16/28

Abstract: In alternative embodiments, provided are compositions, including products of manufacture and kits, and methods, for remotely-controlled and non-invasive manipulation of intracellular nucleic acid expression, genetic processes, function and activity in live cells, e.g., adding functions or changing or adding specificities for immune cells, for monitoring physiologic processes, for the correction of pathological processes and for control of therapeutic outcomes. In alternative embodiments, provided are ultrasound-based thermal or mechanical stimulations, and thermo- or mechano-sensitive protein, either synthetically engineered or natively (endogenously) occurring, integrated to control the production of intracellular nucleic acid and gene expression, e.g., for the expression of biological-active proteins, which can be used, in alternative embodiments, for diagnostic or therapeutic purposes. In alternative embodiments, exemplary thermo- and mechanogenetic systems provided herein allow a deep penetration of stimulation and manipulation in vivo at centimeter-level depth with high spatiotemporal precision.

公开(公告)号：US20180153821A1

公开(公告)日：2018-06-07

申请号：US15737566

申请日：2016-06-07

Applicant: The Regents of the University of California

Inventor： Liangfang Zhang ,  Che-Ming Jack Hu ,  Ronnie H. Fang ,  Brian T. Luk ,  Kuei-Chun Wang ,  Shu Chien

IPC: A61K9/51 ,  A61P7/04 ,  A61P9/00 ,  A61P35/00 ,  A61K31/337

CPC classification number: A61K9/5176 ,  A61K9/5153 ,  A61K9/5169 ,  A61K31/337 ,  A61K47/6901 ,  A61P7/04 ,  A61P9/00 ,  A61P35/00 ,  B82Y5/00 ,  B82Y30/00

Abstract: The present invention relates to prevention and/or treatment of diseases or disorders associated with a damaged or leaky vasculature in a subject. The present invention provides for methods, combinations and pharmaceutical compositions for preventing and/or treating a disease or disorder associated with a damaged or leaky vasculature in a subject, using, inter alia, an effective amount of a nanoparticle comprising an inner core comprising a non-cellular material, an outer surface comprising a cellular membrane derived from a platelet; and optionally an agent for preventing, treating, diagnosing, or prognosing the disease or disorder and/or monitoring prevention or treatment of the disease or disorder. Exemplary diseases or disorders include hemorrhage (bleeding), cardiovascular diseases or disorders, diseases or disorders associated with narrowing of a blood vessel, tumors or cancers.

公开(公告)号：US20220151948A1

公开(公告)日：2022-05-19

申请号：US17590684

申请日：2022-02-01

Applicant: The Regents of the University of California

Inventor： Liangfang Zhang ,  Che-Ming Jack Hu ,  Ronnie H. Fang ,  Brian T. Luk ,  Kuei-Chun Wang ,  Shu Chien

IPC: A61K9/51 ,  A61K47/69 ,  A61P7/04 ,  A61P9/00 ,  A61P35/00 ,  A61K31/337

Abstract: The present invention relates to prevention and/or treatment of diseases or disorders associated with a damaged or leaky vasculature in a subject. The present invention provides for methods, combinations and pharmaceutical compositions for preventing and/or treating a disease or disorder associated with a damaged or leaky vasculature in a subject, using, inter alia, an effective amount of a nanoparticle comprising an inner core comprising a non-cellular material, an outer surface comprising a cellular membrane derived from a platelet; and optionally an agent for preventing, treating, diagnosing, or prognosing the disease or disorder and/or monitoring prevention or treatment of the disease or disorder. Exemplary diseases or disorders include hemorrhage (bleeding), cardiovascular diseases or disorders, diseases or disorders associated with narrowing of a blood vessel, tumors or cancers.

公开(公告)号：US11241394B2

公开(公告)日：2022-02-08

申请号：US15737566

申请日：2016-06-07

Applicant: The Regents of the University of California

Inventor： Liangfang Zhang ,  Che-Ming Jack Hu ,  Ronnie H. Fang ,  Brian T. Luk ,  Kuei-Chun Wang ,  Shu Chien

IPC: A61K9/51 ,  A61K47/69 ,  A61P7/04 ,  A61P9/00 ,  A61P35/00 ,  A61K31/337 ,  B82Y5/00 ,  B82Y30/00

Abstract: The present invention relates to prevention and/or treatment of diseases or disorders associated with a damaged or leaky vasculature in a subject. The present invention provides for methods, combinations and pharmaceutical compositions for preventing and/or treating a disease or disorder associated with a damaged or leaky vasculature in a subject, using, inter alia, an effective amount of a nanoparticle comprising an inner core comprising a non-cellular material, an outer surface comprising a cellular membrane derived from a platelet; and optionally an agent for preventing, treating, diagnosing, or prognosing the disease or disorder and/or monitoring prevention or treatment of the disease or disorder. Exemplary diseases or disorders include hemorrhage (bleeding), cardiovascular diseases or disorders, diseases or disorders associated with narrowing of a blood vessel, tumors or cancers.

公开(公告)号：US11667900B2

公开(公告)日：2023-06-06

申请号：US16631041

申请日：2018-07-12

Applicant: The Regents of the University of California

Inventor： Yingxiao Wang ,  Shu Chien ,  Lei Lei ,  Shaoying Lu ,  Jie Sun

IPC: C12N9/16 ,  A61K38/00 ,  A61K39/395 ,  C07K14/435

CPC classification number: C12N9/16 ,  A61K39/39558 ,  C07K14/435 ,  C12Y301/03048 ,  A61K38/00 ,  C07K2319/03 ,  C07K2319/61

Abstract: Provided are Shp2- and Spleen tyrosine kinase (Syk)-integrated sensing and actuating protein (iSNAP) (Shp2- and Syk-iSNAP) chimeric proteins comprising: a bi-phosphorylatable peptide, optionally a bisphosphoryl tyrosine-based activation (BTAM) motif; a Fluorescent Protein (FP) Förster Resonance Energy Transfer (FRET) (or FP FRET) pair or pair of motifs; a truncated Shp2 domain comprising an N-Src Homology 2(N-SH2) domain and a C-Src Homology 2(C-SH2) domain; and, a phosphatase (PTP) domain or a kinase domain. Provided are engineered cells and methods for cancer cell or tumor eradication, or for the treatment or amelioration of a cancer, tumor or dysfunctional cell, or for promoting an anti-cancer, anti-tumor or anti-dysfunctional cell inflammatory response, including enhancing macrophage-, monocyte-, microglia-, osteoclast-, Kupffer cell- or dendritic cell-mediated antibody- or monoclonal antibody (mAb)-guided cancer or dysfunctional cell or tumor eradication, amelioration, or treatment.