公开(公告)号：US20250163091A1

公开(公告)日：2025-05-22

申请号：US18729672

申请日：2023-01-19

Applicant: The Regents of the University of California

Inventor： Xi CHEN ,  Yuanyuan BAI ,  Hai YU ,  Libo ZHANG ,  Xiaoxiao YANG

IPC: C07H5/06

Abstract: New derivatives of human milk oligosaccharides and methods for preparing them are disclosed. Also described herein are hST6GALNAC V and hGCNT2-B variants, along with polynucleotides encoding the variants and host cells comprising the polynucleotides. Further provided herein are reaction mixtures comprising the hST6GALNAC V and hGCNT2-B variants.

公开(公告)号：US20240011064A1

公开(公告)日：2024-01-11

申请号：US18450181

申请日：2023-08-15

Applicant: The Regents of the University of California

Inventor： Shota ATSUMI ,  Angela ZHANG ,  Xi CHEN ,  Yuanyuan BAI ,  Hai YU ,  John B. McARTHUR

IPC: C12P19/04 ,  C12N9/10 ,  C12N9/12

CPC classification number: C12P19/04 ,  C12N9/1051 ,  C12N9/1241 ,  C12Y204/01069 ,  C12Y207/07064

Abstract: Methods for producing oligosaccharide products such as difucosylated oligosaccharides are disclosed. The methods include culturing recombinant cells in a cell culture medium comprising L-fucose, an oligosaccharide acceptor, and a carbon source. The cells are cultured under conditions in which a first fucosyltransferase polypeptide having a first substrate selectivity (e.g., an α1-2-fucosyltransferase polypeptide), a second fucosyltransferase polypeptide having a second substrate specificity (e.g., an α1-3-fucosyltransferase polypeptide), a nucleotide sugar pyrophosphorylase polypeptide, a lactose transporter polypeptide, and an L-fucose transporter polypeptide are expressed, and in which the oligosaccharide acceptor is converted to the difucosylated oligosaccharide. Recombinant cells for use in the methods are also described.