公开(公告)号：US20240409571A1

公开(公告)日：2024-12-12

申请号：US18627308

申请日：2024-04-04

Applicant: The Regents of the University of California

Inventor： Xi CHEN ,  Hai YU ,  Bijoyananda MISHRA ,  Zimin ZHENG ,  Anand Kumar AGRAHARI ,  Arin GUCCHAIT

IPC: C07H15/12 ,  C07H1/00 ,  C12P13/00

Abstract: Described herein are novel stable 9-N-, 8-N-, and 7-N-acetyl analogues of instable 9-O-acetyl, 8-O-acetyl, and 7-O-acetyl b-series gangliosides and glycosphingosines, including GD3, GD2, GD1b, GT1b, GQ1b, and their glycosphingosines. Chemoenzymatic methods for the production of the stable 9-N-, 8-N-, and 7-N-acetyl analogues are also described herein.

公开(公告)号：US20210040458A1

公开(公告)日：2021-02-11

申请号：US16906898

申请日：2020-06-19

Applicant: The Regents of the University of California

Inventor： Xi CHEN ,  Lan NA ,  Hai YU ,  John B. McARTHUR

IPC: C12N9/10 ,  C12P19/04

Abstract: Heparosan synthase variants having improved expression levels, enhanced thermal stability, and/or reduced reverse glycosylation activity are provided. Methods for making oligosaccharides and polysaccharides, including heparin analogs and heparan sulfate analogs, are also described.

公开(公告)号：US20180371432A1

公开(公告)日：2018-12-27

申请号：US16062883

申请日：2016-12-19

Applicant: The Regents of the University of California

Inventor： Xi CHEN ,  Chao ZHAO ,  Hai YU

IPC: C12N9/10 ,  C12P19/18 ,  C12P19/26 ,  C12P19/44 ,  C12N15/70

Abstract: α1-2-fucosyltransferases, and methods and compositions for making and using α1-2-fucosyltransferases, are described herein.

公开(公告)号：US20250163091A1

公开(公告)日：2025-05-22

申请号：US18729672

申请日：2023-01-19

Applicant: The Regents of the University of California

Inventor： Xi CHEN ,  Yuanyuan BAI ,  Hai YU ,  Libo ZHANG ,  Xiaoxiao YANG

IPC: C07H5/06

Abstract: New derivatives of human milk oligosaccharides and methods for preparing them are disclosed. Also described herein are hST6GALNAC V and hGCNT2-B variants, along with polynucleotides encoding the variants and host cells comprising the polynucleotides. Further provided herein are reaction mixtures comprising the hST6GALNAC V and hGCNT2-B variants.

公开(公告)号：US20220145343A1

公开(公告)日：2022-05-12

申请号：US17429299

申请日：2020-02-07

Applicant: The Regents of the University of California

Inventor： Xi CHEN ,  Riyao LI ,  Hai YU

IPC: C12P19/04 ,  A61K39/095 ,  A61K39/102 ,  A61K39/09 ,  A61K39/108

Abstract: Methods for preparing saccharide products such as bacterial capsular polysaccharides are provided. The methods include: forming a reaction mixture containing one or more bacterial capsular polysaccharide synthases, a sugar acceptor, and one or more sugar donors; and maintaining the reaction mixture under conditions sufficient to form the bacterial capsular saccharide product. Vaccine compositions containing bacterial capsular saccharide products prepared according to the methods are also described.

公开(公告)号：US20240011064A1

公开(公告)日：2024-01-11

申请号：US18450181

申请日：2023-08-15

Applicant: The Regents of the University of California

Inventor： Shota ATSUMI ,  Angela ZHANG ,  Xi CHEN ,  Yuanyuan BAI ,  Hai YU ,  John B. McARTHUR

IPC: C12P19/04 ,  C12N9/10 ,  C12N9/12

CPC classification number: C12P19/04 ,  C12N9/1051 ,  C12N9/1241 ,  C12Y204/01069 ,  C12Y207/07064

Abstract: Methods for producing oligosaccharide products such as difucosylated oligosaccharides are disclosed. The methods include culturing recombinant cells in a cell culture medium comprising L-fucose, an oligosaccharide acceptor, and a carbon source. The cells are cultured under conditions in which a first fucosyltransferase polypeptide having a first substrate selectivity (e.g., an α1-2-fucosyltransferase polypeptide), a second fucosyltransferase polypeptide having a second substrate specificity (e.g., an α1-3-fucosyltransferase polypeptide), a nucleotide sugar pyrophosphorylase polypeptide, a lactose transporter polypeptide, and an L-fucose transporter polypeptide are expressed, and in which the oligosaccharide acceptor is converted to the difucosylated oligosaccharide. Recombinant cells for use in the methods are also described.

公开(公告)号：US20220106348A1

公开(公告)日：2022-04-07

申请号：US17491518

申请日：2021-09-30

Applicant: The Regents of the University of California

Inventor： Xi CHEN ,  An XIAO ,  Anoopjit KOONER ,  Abhishek SANTRA ,  Ajit VARKI ,  Hai YU ,  Lee-Ping WANG ,  Wanqing LI

IPC: C07H15/04 ,  C07H15/12 ,  C07H15/14 ,  C07H15/203 ,  C12P19/28 ,  C12P19/44 ,  C12P19/64

Abstract: The present invention provides N-acetyl derivatives of sialic acids, including N-acetyl derivatives of Neu5Ac and Neu5Gc. Methods for preparing related precursors and a variety of sialosides are also disclosed.