公开(公告)号：US20180223326A1

公开(公告)日：2018-08-09

申请号：US15579953

申请日：2016-06-17

申请人： The Research Foundation For Microbial Diseases Of Osaka University ,  Osaka University

发明人： Yukihiro Akeda ,  Hiroshi Miyatake ,  Zhenyu Piao ,  Yuka Koizumi

IPC分类号： C12Q1/18

CPC分类号： C12Q1/18 ,  C07K16/12 ,  C12N1/20 ,  G01N2333/3156

摘要： The present invention relates to a measurement method for complement-dependent bactericidal activity against Streptococcus pneumoniae, and provides a measurement method capable of measuring complement-dependent bactericidal activity against Streptococcus pneumoniae of any capsular serotype. Complement-dependent bactericidal activity against Streptococcus pneumoniae is measured using capsule-deficient Streptococcus pneumoniae, that is, non-encapsulated or substantially non-encapsulated, or transparent Streptococcus pneumoniae. The measurement of the complement-dependent bactericidal activity against Streptococcus pneumoniae of any capsular serotype is enabled.

公开(公告)号：US09938326B2

公开(公告)日：2018-04-10

申请号：US14429547

申请日：2013-03-22

申请人： Osaka University

发明人： Yukihiro Akeda ,  Zhenyu Piao ,  Kazunori Oishi

IPC分类号： A61K45/00 ,  C07K14/315 ,  A61K39/09 ,  A61K39/00 ,  A61K38/00

CPC分类号： C07K14/3156 ,  A61K38/00 ,  A61K39/092 ,  A61K2039/54 ,  A61K2039/55505 ,  A61K2039/55561 ,  A61K2039/70 ,  C07K2319/00 ,  C07K2319/705

摘要： A pneumococcal vaccine comprising a fusion protein at least comprising a full-length family 1 pneumococcal surface protein A (PspA) or a fragment thereof, and a full-length family 2 PspA or a fragment thereof, in particular any one of the following fusion proteins (1) to (3): (1) a fusion protein at least comprising a family 1, clade 2 PspA and a family 2, clade 3 PspA, (2) a fusion protein at least comprising a family 1, clade 2 PspA and a family 2, clade 4 PspA, and (3) a fusion protein at least comprising a family 1, clade 2 PspA and a family 2, clade 5 PspA, is useful as a pneumococcal vaccine comprising a single protein antigen that has broadly cross-reactive immunogenicity and can induce immune response against a wide range of pneumococcal clinical isolates.

公开(公告)号：US20150320851A1

公开(公告)日：2015-11-12

申请号：US14429547

申请日：2013-03-22

申请人： OSAKA UNIVERSITY

发明人： Yukihiro Akeda ,  Zhenyu Piao ,  Kazunori Oishi

IPC分类号： A61K39/09

CPC分类号： C07K14/3156 ,  A61K38/00 ,  A61K39/092 ,  A61K2039/54 ,  A61K2039/55505 ,  A61K2039/55561 ,  A61K2039/70 ,  C07K2319/00 ,  C07K2319/705

摘要： A pneumococcal vaccine comprising a fusion protein at least comprising a full-length family 1 pneumococcal surface protein A (PspA) or a fragment thereof, and a full-length family 2 PspA or a fragment thereof, in particular any one of the following fusion proteins (1) to (3): (1) a fusion protein at least comprising a family 1, clade 2 PspA and a family 2, clade 3 PspA, (2) a fusion protein at least comprising a family 1, clade 2 PspA and a family 2, clade 4 PspA, and (3) a fusion protein at least comprising a family 1, clade 2 PspA and a family 2, clade 5 PspA, is useful as a pneumococcal vaccine comprising a single protein antigen that has broadly cross-reactive immunogenicity and can induce immune response against a wide range of pneumococcal clinical isolates.

摘要翻译： 包含至少包含全长家族1肺炎球菌表面蛋白A（PspA）或其片段和全长家族2 PspA或其片段的融合蛋白的肺炎球菌疫苗，特别是以下融合蛋白 （1）至（3）所述的融合蛋白：（1）至少包含家族1，进化枝2 PspA和家族2，进化枝3 PspA的融合蛋白，（2）至少包含家族1，进化枝2 PspA和 家族2，进化枝4 PspA，和（3）至少包含家族1，进化枝2 PspA和家族2，进化枝5PspA的融合蛋白，可用作肺炎球菌疫苗，其包含单一蛋白抗原， 反应性免疫原性，并能诱导针对广泛的肺炎球菌临床分离株的免疫应答。