公开(公告)号：US20240327825A1

公开(公告)日：2024-10-03

申请号：US18745866

申请日：2024-06-17

申请人： Universität Zürich

发明人： Markus SEEGER ,  Pascal EGLOFF ,  Iwan ZIMMERMANN

IPC分类号： C12N15/10 ,  C07K16/12 ,  G01N33/68

CPC分类号： C12N15/1065 ,  C07K16/12 ,  C12N15/1034 ,  C12N15/1093 ,  G01N33/6845 ,  G01N33/6848 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/23 ,  C07K2319/24 ,  C07K2319/41 ,  C07K2319/43 ,  C12Q2563/119 ,  C12Q2563/185

摘要： The invention relates to a method for identifying and quantifying a polypeptide from a library of polypeptides. The method comprises the steps of: 1—providing a polypeptide library and a detection tag library, 2—generating a nested library comprising the polypeptides and the detection tags, 3—sequencing the nested library, 4—selecting a member of the nested library in one or several selection steps that are independent of a physical genotype-phenotype linkage, 5—isolating the detection tag from the selected polypeptide, 6—identifying and quantifying the detection tag by mass spectrometry, 7—obtaining the sequence of the selected polypeptide. The invention also relates to a collection of polypeptides, a collection of detection tags, and a collection of plasmid vectors.

公开(公告)号：US20240309074A1

公开(公告)日：2024-09-19

申请号：US18467204

申请日：2023-09-14

申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Donald E. INGBER ,  Michael SUPER ,  Jeffrey Charles WAY ,  Mark J. CARTWRIGHT ,  Julia B. BERTHET ,  Dinah R. SUPER ,  Martin ROTTMAN ,  Alexander L WATTERS

IPC分类号： C07K16/12 ,  A61K47/68 ,  C07K14/42 ,  G01N33/543 ,  G01N33/569

CPC分类号： C07K16/12 ,  A61K47/68 ,  A61K47/6815 ,  C07K14/42 ,  G01N33/54353 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56961 ,  C07K2319/33 ,  G01N2333/42 ,  G01N2333/4724 ,  Y02A50/30

摘要： Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

公开(公告)号：US20240182529A1

公开(公告)日：2024-06-06

申请号：US16955653

申请日：2018-11-30

申请人： BEIJING ZEQIN BIOMEDICAL CO., LTD

发明人： Huawei WEI ,  Chenggang YANG

IPC分类号： C07K14/195 ,  A61P35/00 ,  C07K16/12 ,  A61K39/00

CPC分类号： C07K14/195 ,  A61P35/00 ,  C07K16/12 ,  A61K2039/505

摘要： An improved pH low insertion peptide which is obtained by repeating the sequence of the extracellular domain of a pH low insertion peptide one or more times based on the sequence of the pH low insertion peptide already existing in the prior art. The pH low insertion peptide uses tumor cells cultured in vitro to prove that the improved pH low insertion peptide is targeted to the surface of tumor cells. Also disclosed are compositions composed of the pH low insertion peptide or the improved type thereof, and these compositions can be used for tumor treatment, diagnosis and identification. The experiments also find that the extracellular domain region of the pH low insertion peptide or the improved type thereof has antigenicity, and the immunized antibody can be used for tumor treatment. The above research results provide a basis for the development of tumor therapeutic drugs.

公开(公告)号：US20230382996A1

公开(公告)日：2023-11-30

申请号：US18164952

申请日：2023-02-06

申请人： MacroGenics, Inc.

发明人： Leslie S. Johnson ,  Ling Huang

IPC分类号： C07K16/28 ,  C07K16/08 ,  C07K16/12 ,  C07K16/30 ,  C07K16/44

CPC分类号： C07K16/283 ,  C07K16/08 ,  C07K16/12 ,  C07K16/30 ,  C07K16/44 ,  C07K2317/52 ,  A61K2039/505

摘要： The present invention is directed to diabody molecules and uses thereof in the treatment of a variety of diseases and disorders, including immunological disorders, infectious disease, intoxication and cancers. The diabody molecules of the invention comprise two polypeptide chains that associate to form at least two epitope binding sites, which may recognize the same or different epitopes on the same or differing antigens. Additionally, the antigens may be from the same or different molecules. The individual polypeptide chains of the diabody molecule may be covalently bound through non-peptide bond covalent bonds, such as, but not limited to, disulfide bonding of cysteine residues located within each polypeptide chain. In particular embodiments, the diabody molecules of the present invention further comprise an Fc region, which allows antibody-like functionality to engineered into the molecule.

公开(公告)号：US20230382980A1

公开(公告)日：2023-11-30

申请号：US17820406

申请日：2022-08-17

申请人： The Brigham and Women's Hospital, Inc. ,  Beth Israel Deaconess Medical Center, Inc.

发明人： Gerald B. Pier ,  Casie Anne Kelly-Quintos ,  Lisa Cavacini ,  Marshall R. Posner

IPC分类号： C07K16/12 ,  A61K39/00

CPC分类号： C07K16/1271 ,  C07K16/12 ,  C07K2317/565 ,  A61K2039/505 ,  C07K2317/73 ,  Y02A50/30 ,  C07K2317/56 ,  C07K2317/21

摘要： The present invention relates to peptides, particularly human monoclonal antibodies, that bind specifically to poly-N-acetyl glucosamine (PNAG), such as Staphylococcal PNAG, in acetylated, partially acetylated and/or fully deacetylated form. The invention further provides methods for using these peptides in the diagnosis, prophylaxis and therapy of infections by bacteria that express PNAG such as but not limited to Staphylococci and E. coli. Some antibodies of the invention enhance opsonophagocytic killing and in vivo protection against bacteria that express PNAG such as but not limited to Staphylococci and E. coli. Compositions of these peptides, including pharmaceutical compositions, are also provided, as are functionally equivalent variants of such peptides.

公开(公告)号：US11684673B2

公开(公告)日：2023-06-27

申请号：US17150731

申请日：2021-01-15

申请人： RESEARCH INSTITUTE AT NATIONWIDE CHILDREN'S HOSPITAL

发明人： Lauren O. Bakaletz ,  Steven D. Goodman

IPC分类号： A61K39/40 ,  C07K16/18 ,  C12N15/62 ,  A61L27/54 ,  A61L29/16 ,  A61Q11/00 ,  A61Q17/00 ,  C07K16/12 ,  A61K8/64 ,  A61K39/395 ,  A61K39/12 ,  A61P31/04 ,  A61K39/00

CPC分类号： A61K39/40 ,  A61K8/64 ,  A61K39/12 ,  A61K39/395 ,  A61L27/54 ,  A61L29/16 ,  A61P31/04 ,  A61Q11/00 ,  A61Q17/005 ,  C07K16/12 ,  C07K16/1217 ,  C07K16/1232 ,  C07K16/1242 ,  C07K16/1271 ,  C07K16/1275 ,  C07K16/18 ,  C12N15/62 ,  A61K2039/507 ,  A61L2300/404 ,  C07K2317/34 ,  C07K2317/76

摘要： This disclosure provides isolated or recombinant polypeptides that are useful to vaccinate individuals suffering from chronic/recurrent biofilm disease or as a therapeutic for those with an existing infection. The individual's immune system will then naturally generate antibodies which prevent or clear these bacteria from the host by interfering with the construction and or maintenance of a functional protective biofilm. Alternatively, antibodies to the polypeptides can be administered to treat or prevent infection. Bacteria that are released from the biofilm by our technology are more readily cleared by the remainder of the host's immune system.

公开(公告)号：US20180246096A1

公开(公告)日：2018-08-30

申请号：US15970391

申请日：2018-05-03

申请人： The Trustees of Columbia University in the City of New York

发明人： Yiping HAN

IPC分类号： G01N33/569 ,  G01N33/574 ,  C07K16/12

CPC分类号： G01N33/56916 ,  C07K16/12 ,  C12Q1/689 ,  G01N33/56911 ,  G01N33/57419 ,  G01N33/57484 ,  G01N2469/20 ,  G01N2800/065 ,  G01N2800/368 ,  G01N2800/60

摘要： The present invention relates to detecting serum anti-FadA antibodies in test samples from a patient. Additionally, aspects of the present invention provide the basis for detection of serum anti-FadA antibody levels from test samples and correlation with various conditions of clinical relevance.

公开(公告)号：US20180164316A1

公开(公告)日：2018-06-14

申请号：US15577542

申请日：2016-05-27

申请人： DENKA SEIKEN CO., LTD.

发明人： Risa KOHIYAMA ,  Takashi MIYAZAWA

IPC分类号： G01N33/577 ,  G01N33/569

CPC分类号： G01N33/577 ,  C07K16/12 ,  C12N15/02 ,  G01N33/543 ,  G01N33/569 ,  G01N33/56933 ,  G01N2333/30

摘要： Disclosed is a novel method of detecting a test subject by an immunoassay, in which low sensitivity due to using a monoclonal antibody is improved in spite of using a monoclonal antibody in the immunoassay. The method of detecting a test subject is a method of detecting a test subject having two or more kinds of antigens which enable detection of said test subject, which method is performed by an immunoassay such as a sandwich method, using a monoclonal antibody or an antigen-binding fragment thereof which recognizes the two or more kinds of antigens.

公开(公告)号：US20170368113A1

公开(公告)日：2017-12-28

申请号：US15631216

申请日：2017-06-23

申请人： IgY Nutrition

发明人： Ainslee Crum ,  Susan J. Hewlings

IPC分类号： A61K35/747 ,  A61K35/745 ,  A61K35/741 ,  A61K35/742 ,  C07K16/12 ,  A61K39/02

CPC分类号： A61K35/747 ,  A61K35/57 ,  A61K35/741 ,  A61K35/742 ,  A61K35/745 ,  A61K39/02 ,  C07K16/12

摘要： Methods for treating and preventing dysbiosis in a subject suffering from or susceptible to gastrointestinal disorders are described. The methods comprises administering a hyperimmunized egg product to the subject, wherein the egg product is obtained from a hyperimmunized avian, optionally in combination with an additional compound such as a probiotic and/or nutrient source.

公开(公告)号：US20170327860A1

公开(公告)日：2017-11-16

申请号：US15496807

申请日：2017-04-25

申请人： Merus N.V.

发明人： Cornelis Adriaan DE KRUIF ,  Linda Johanna Aleida HENDRIKS ,  Ton LOGTENBERG

IPC分类号： C12P21/00 ,  A61K39/395 ,  A61K39/40 ,  C07K16/12 ,  C07K16/46 ,  C07K16/36 ,  C07K16/28 ,  C07K16/26 ,  C07K16/18 ,  A61K39/00

CPC分类号： C12P21/005 ,  A61K39/395 ,  A61K39/3955 ,  A61K39/40 ,  A61K2039/507 ,  C07K16/12 ,  C07K16/1278 ,  C07K16/1282 ,  C07K16/18 ,  C07K16/26 ,  C07K16/28 ,  C07K16/2809 ,  C07K16/283 ,  C07K16/2866 ,  C07K16/2896 ,  C07K16/36 ,  C07K16/468 ,  C07K2317/14 ,  C07K2317/31 ,  C07K2317/526 ,  C07K2317/56 ,  C07K2317/94

摘要： The invention provides means and methods for producing one or more Ig-like molecules in a single host cell. Novel CH3 mutations enabling the production of monospecific and/or bispecific Ig-like molecules of interest are also provided.