公开(公告)号：US20250146089A1

公开(公告)日：2025-05-08

申请号：US18936289

申请日：2024-11-04

Applicant: The Trustees of Princeton University

Inventor： Caitlin Lamb ,  Arend Jan Wouter te Velthuis ,  Cameron Myhrvold

IPC: C12Q1/70 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6818

Abstract: Disclosed is a method for quantitative detection of a small, aberrant viral nucleic acid. The method includes combining a detection mixture with a sample, and allowing a reaction with the test mixture to begin. The sample includes an RNA sample. The detection mixture includes a Cas13 enzyme, a reporter, a sequence-specific CRISPR RNA (crRNA), and a buffer. The method includes determining a quantitative value representative of the reporter in the test mixture, where the quantitative value is a measure of an amount of target RNA present in the RNA sample.